Relationship between methylation and colonic inflammation in inflammatory bowel disease

AIM: To investigate the relationship between the methylation status in the SLIT2 and TGFB2 promoters and colonic inflammation in inflammatory bowel disease patients. METHODS: We evaluated the methylation status of 2 genes (SLIT2 and TGFB2) in 226 biopsies taken from 62 colonoscopies of 38 patients (...

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Autores: Lobatón, Triana, Azuara García, Daniel, Rodríguez Moranta, Francisco, Loayza, Carolina, Sanjuan, Xavier, Oca Burguete, Javier de, Fernández-Robles, Ana, Guardiola, Jordi, Capellá, G. (Gabriel)
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2014
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/189206
Acceso en línea:https://hdl.handle.net/2445/189206
Access Level:acceso abierto
Palabra clave:Inflamació
Colitis
Càncer colorectal
Malalties del còlon
Inflammation
Colorectal cancer
Colonic diseases
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spelling Relationship between methylation and colonic inflammation in inflammatory bowel diseaseLobatón, TrianaAzuara García, DanielRodríguez Moranta, FranciscoLoayza, CarolinaSanjuan, XavierOca Burguete, Javier deFernández-Robles, AnaGuardiola, JordiCapellá, G. (Gabriel)InflamacióColitisCàncer colorectalMalalties del còlonInflammationColitisColorectal cancerColonic diseasesAIM: To investigate the relationship between the methylation status in the SLIT2 and TGFB2 promoters and colonic inflammation in inflammatory bowel disease patients. METHODS: We evaluated the methylation status of 2 genes (SLIT2 and TGFB2) in 226 biopsies taken from 62 colonoscopies of 38 patients (29 ulcerative colitis and 9 Crohn's colitis) using methylation-specific melting curve analysis. The relationships between methylation status and clinical, biological, endoscopic and histological activities were evaluated. Twenty-three of the 38 patients had a second colonoscopy and were included in a longitudinal analysis. Numerical results were given as the means ± SD of the sample and range, except when specified. Student t analysis, U Mann Whitney and ANOVA factor were used to compare the means. Qualitative results were based on the χ2 test. RESULTS: SLIT2 methylation was more frequent in samples with endoscopic activity than with endoscopic remission (55% vs 18%, P < 0.001). SLIT2 methylation was also higher in samples with acute inflammation (56.5%) than in samples with chronic (24%) or absent inflammation (15%) (P < 0.001). For TGFB2 methylation, the correlation was only significant with endoscopic activity. Methylation was higher in the distal colon for both genes (P < 0.001 for SLIT2 and P = 0.022 for TGFB2). In the multivariate analysis, only inflammation status (and not disease duration or extension) was independently associated with SLIT2 methylation [OR = 6.6 (95%CI: 1.65-27.36), P = 0.009]. In the longitudinal analysis, the maintenance of endoscopic remission was protective for methylation.Baishideng Publishing Group Inc2014info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/189206Articles publicats en revistes (Ciències Clíniques)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.3748/wjg.v20.i30.10591World Journal of Gastroenterology, 2014, vol. 20, num. 30, p. 10591-10598https://doi.org/10.3748/wjg.v20.i30.10591cc-by-nc (c) Lobatón, Triana et al., 2014https://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1892062026-05-27T06:46:51Z
dc.title.none.fl_str_mv Relationship between methylation and colonic inflammation in inflammatory bowel disease
title Relationship between methylation and colonic inflammation in inflammatory bowel disease
spellingShingle Relationship between methylation and colonic inflammation in inflammatory bowel disease
Lobatón, Triana
Inflamació
Colitis
Càncer colorectal
Malalties del còlon
Inflammation
Colitis
Colorectal cancer
Colonic diseases
title_short Relationship between methylation and colonic inflammation in inflammatory bowel disease
title_full Relationship between methylation and colonic inflammation in inflammatory bowel disease
title_fullStr Relationship between methylation and colonic inflammation in inflammatory bowel disease
title_full_unstemmed Relationship between methylation and colonic inflammation in inflammatory bowel disease
title_sort Relationship between methylation and colonic inflammation in inflammatory bowel disease
dc.creator.none.fl_str_mv Lobatón, Triana
Azuara García, Daniel
Rodríguez Moranta, Francisco
Loayza, Carolina
Sanjuan, Xavier
Oca Burguete, Javier de
Fernández-Robles, Ana
Guardiola, Jordi
Capellá, G. (Gabriel)
author Lobatón, Triana
author_facet Lobatón, Triana
Azuara García, Daniel
Rodríguez Moranta, Francisco
Loayza, Carolina
Sanjuan, Xavier
Oca Burguete, Javier de
Fernández-Robles, Ana
Guardiola, Jordi
Capellá, G. (Gabriel)
author_role author
author2 Azuara García, Daniel
Rodríguez Moranta, Francisco
Loayza, Carolina
Sanjuan, Xavier
Oca Burguete, Javier de
Fernández-Robles, Ana
Guardiola, Jordi
Capellá, G. (Gabriel)
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Inflamació
Colitis
Càncer colorectal
Malalties del còlon
Inflammation
Colitis
Colorectal cancer
Colonic diseases
topic Inflamació
Colitis
Càncer colorectal
Malalties del còlon
Inflammation
Colitis
Colorectal cancer
Colonic diseases
description AIM: To investigate the relationship between the methylation status in the SLIT2 and TGFB2 promoters and colonic inflammation in inflammatory bowel disease patients. METHODS: We evaluated the methylation status of 2 genes (SLIT2 and TGFB2) in 226 biopsies taken from 62 colonoscopies of 38 patients (29 ulcerative colitis and 9 Crohn's colitis) using methylation-specific melting curve analysis. The relationships between methylation status and clinical, biological, endoscopic and histological activities were evaluated. Twenty-three of the 38 patients had a second colonoscopy and were included in a longitudinal analysis. Numerical results were given as the means ± SD of the sample and range, except when specified. Student t analysis, U Mann Whitney and ANOVA factor were used to compare the means. Qualitative results were based on the χ2 test. RESULTS: SLIT2 methylation was more frequent in samples with endoscopic activity than with endoscopic remission (55% vs 18%, P < 0.001). SLIT2 methylation was also higher in samples with acute inflammation (56.5%) than in samples with chronic (24%) or absent inflammation (15%) (P < 0.001). For TGFB2 methylation, the correlation was only significant with endoscopic activity. Methylation was higher in the distal colon for both genes (P < 0.001 for SLIT2 and P = 0.022 for TGFB2). In the multivariate analysis, only inflammation status (and not disease duration or extension) was independently associated with SLIT2 methylation [OR = 6.6 (95%CI: 1.65-27.36), P = 0.009]. In the longitudinal analysis, the maintenance of endoscopic remission was protective for methylation.
publishDate 2014
dc.date.none.fl_str_mv 2014
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/189206
url https://hdl.handle.net/2445/189206
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.3748/wjg.v20.i30.10591
World Journal of Gastroenterology, 2014, vol. 20, num. 30, p. 10591-10598
https://doi.org/10.3748/wjg.v20.i30.10591
dc.rights.none.fl_str_mv cc-by-nc (c) Lobatón, Triana et al., 2014
https://creativecommons.org/licenses/by-nc/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by-nc (c) Lobatón, Triana et al., 2014
https://creativecommons.org/licenses/by-nc/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Baishideng Publishing Group Inc
publisher.none.fl_str_mv Baishideng Publishing Group Inc
dc.source.none.fl_str_mv Articles publicats en revistes (Ciències Clíniques)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300719

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