Combined fluorescence and reflectance spectroscopy for in vivo quantification of cancer biomarkers in low - and high- grade glioma surgery
Biomarkers are indicators of biological processes and hold promise for the diagnosis and treatment of disease. Gliomas represent a heterogeneous group of brain tumors with marked intra- and inter-tumor variability. The extent of surgical resection is a significant factor influencing post-surgical re...
| Autores: | , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Fecha de publicación: | 2011 |
| País: | España |
| Institución: | Universidad de Cantabria (UC) |
| Repositorio: | UCrea Repositorio Abierto de la Universidad de Cantabria |
| Idioma: | inglés |
| OAI Identifier: | oai:repositorio.unican.es:10902/1734 |
| Acceso en línea: | http://hdl.handle.net/10902/1734 |
| Access Level: | acceso abierto |
| Palabra clave: | 5-aminolevulinic acid Protoporphyrin IX Fluorescence-guided resection Optical spectroscopy Glioma Cancer biomarkers |
| id |
ES_c93795a6af4c19f54110114968ea2538 |
|---|---|
| oai_identifier_str |
oai:repositorio.unican.es:10902/1734 |
| network_acronym_str |
ES |
| network_name_str |
España |
| repository_id_str |
|
| spelling |
Combined fluorescence and reflectance spectroscopy for in vivo quantification of cancer biomarkers in low - and high- grade glioma surgeryValdés, Pablo A.Kim, AnthonyLeblond, FredericConde Portilla, Olga María|||0000-0002-2471-3051Harris, Brent T.Paulsen, Keith D.Wilson, Brian C.Roberts, David W.5-aminolevulinic acidProtoporphyrin IXFluorescence-guided resectionOptical spectroscopyGliomaCancer biomarkersBiomarkers are indicators of biological processes and hold promise for the diagnosis and treatment of disease. Gliomas represent a heterogeneous group of brain tumors with marked intra- and inter-tumor variability. The extent of surgical resection is a significant factor influencing post-surgical recurrence and prognosis. Here, we used fluorescence and reflectance spectral signatures for in vivo quantification of multiple biomarkers during glioma surgery, with fluorescence contrast provided by exogenously-induced protoporphyrin IX (PpIX) following administration of 5-aminolevulinic acid. We performed light-transport modeling to quantify multiple biomarkers indicative of tumor biological processes, including the local concentration of PpIX and associated photoproducts, total hemoglobin concentration, oxygen saturation, and optical scattering parameters.We developed a diagnostic algorithm for intra-operative tissue delineation that accounts for the combined tumor-specific predictive capabilities of these quantitative biomarkers. Tumor tissue delineation achieved accuracies of up to 94% (specificity=94%, sensitivity=94%) across a range of glioma histologies beyond current state-of-the-art optical approaches, including state-of-the-art fluorescence image guidance. This multiple biomarker strategy opens the door to optical methods for surgical guidance that use quantification of well-established neoplastic processes. Future work would seek to validate the predictive power of this proof-of-concept study in a separate larger cohort of patients.SPIE Society of Photo-Optical Instrumentation EngineersUniversidad de Cantabria20112011-11-01journal articlehttp://purl.org/coar/resource_type/c_6501NAhttp://purl.org/coar/version/c_be7fb7dd8ff6fe43info:eu-repo/semantics/articlehttp://hdl.handle.net/10902/1734Journal of Biomedical Optics, 2011, 16(11), 116007reponame:UCrea Repositorio Abierto de la Universidad de Cantabriainstname:Universidad de Cantabria (UC)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:repositorio.unican.es:10902/17342026-06-02T12:39:31Z |
| dc.title.none.fl_str_mv |
Combined fluorescence and reflectance spectroscopy for in vivo quantification of cancer biomarkers in low - and high- grade glioma surgery |
| title |
Combined fluorescence and reflectance spectroscopy for in vivo quantification of cancer biomarkers in low - and high- grade glioma surgery |
| spellingShingle |
Combined fluorescence and reflectance spectroscopy for in vivo quantification of cancer biomarkers in low - and high- grade glioma surgery Valdés, Pablo A. 5-aminolevulinic acid Protoporphyrin IX Fluorescence-guided resection Optical spectroscopy Glioma Cancer biomarkers |
| title_short |
Combined fluorescence and reflectance spectroscopy for in vivo quantification of cancer biomarkers in low - and high- grade glioma surgery |
| title_full |
Combined fluorescence and reflectance spectroscopy for in vivo quantification of cancer biomarkers in low - and high- grade glioma surgery |
| title_fullStr |
Combined fluorescence and reflectance spectroscopy for in vivo quantification of cancer biomarkers in low - and high- grade glioma surgery |
| title_full_unstemmed |
Combined fluorescence and reflectance spectroscopy for in vivo quantification of cancer biomarkers in low - and high- grade glioma surgery |
| title_sort |
Combined fluorescence and reflectance spectroscopy for in vivo quantification of cancer biomarkers in low - and high- grade glioma surgery |
| dc.creator.none.fl_str_mv |
Valdés, Pablo A. Kim, Anthony Leblond, Frederic Conde Portilla, Olga María|||0000-0002-2471-3051 Harris, Brent T. Paulsen, Keith D. Wilson, Brian C. Roberts, David W. |
| author |
Valdés, Pablo A. |
| author_facet |
Valdés, Pablo A. Kim, Anthony Leblond, Frederic Conde Portilla, Olga María|||0000-0002-2471-3051 Harris, Brent T. Paulsen, Keith D. Wilson, Brian C. Roberts, David W. |
| author_role |
author |
| author2 |
Kim, Anthony Leblond, Frederic Conde Portilla, Olga María|||0000-0002-2471-3051 Harris, Brent T. Paulsen, Keith D. Wilson, Brian C. Roberts, David W. |
| author2_role |
author author author author author author author |
| dc.contributor.none.fl_str_mv |
Universidad de Cantabria |
| dc.subject.none.fl_str_mv |
5-aminolevulinic acid Protoporphyrin IX Fluorescence-guided resection Optical spectroscopy Glioma Cancer biomarkers |
| topic |
5-aminolevulinic acid Protoporphyrin IX Fluorescence-guided resection Optical spectroscopy Glioma Cancer biomarkers |
| description |
Biomarkers are indicators of biological processes and hold promise for the diagnosis and treatment of disease. Gliomas represent a heterogeneous group of brain tumors with marked intra- and inter-tumor variability. The extent of surgical resection is a significant factor influencing post-surgical recurrence and prognosis. Here, we used fluorescence and reflectance spectral signatures for in vivo quantification of multiple biomarkers during glioma surgery, with fluorescence contrast provided by exogenously-induced protoporphyrin IX (PpIX) following administration of 5-aminolevulinic acid. We performed light-transport modeling to quantify multiple biomarkers indicative of tumor biological processes, including the local concentration of PpIX and associated photoproducts, total hemoglobin concentration, oxygen saturation, and optical scattering parameters.We developed a diagnostic algorithm for intra-operative tissue delineation that accounts for the combined tumor-specific predictive capabilities of these quantitative biomarkers. Tumor tissue delineation achieved accuracies of up to 94% (specificity=94%, sensitivity=94%) across a range of glioma histologies beyond current state-of-the-art optical approaches, including state-of-the-art fluorescence image guidance. This multiple biomarker strategy opens the door to optical methods for surgical guidance that use quantification of well-established neoplastic processes. Future work would seek to validate the predictive power of this proof-of-concept study in a separate larger cohort of patients. |
| publishDate |
2011 |
| dc.date.none.fl_str_mv |
2011 2011-11-01 |
| dc.type.none.fl_str_mv |
journal article http://purl.org/coar/resource_type/c_6501 NA http://purl.org/coar/version/c_be7fb7dd8ff6fe43 |
| dc.type.openaire.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| dc.identifier.none.fl_str_mv |
http://hdl.handle.net/10902/1734 |
| url |
http://hdl.handle.net/10902/1734 |
| dc.language.none.fl_str_mv |
Inglés eng |
| language_invalid_str_mv |
Inglés |
| language |
eng |
| dc.rights.none.fl_str_mv |
open access http://purl.org/coar/access_right/c_abf2 |
| dc.rights.openaire.fl_str_mv |
info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
open access http://purl.org/coar/access_right/c_abf2 |
| eu_rights_str_mv |
openAccess |
| dc.publisher.none.fl_str_mv |
SPIE Society of Photo-Optical Instrumentation Engineers |
| publisher.none.fl_str_mv |
SPIE Society of Photo-Optical Instrumentation Engineers |
| dc.source.none.fl_str_mv |
Journal of Biomedical Optics, 2011, 16(11), 116007 reponame:UCrea Repositorio Abierto de la Universidad de Cantabria instname:Universidad de Cantabria (UC) |
| instname_str |
Universidad de Cantabria (UC) |
| reponame_str |
UCrea Repositorio Abierto de la Universidad de Cantabria |
| collection |
UCrea Repositorio Abierto de la Universidad de Cantabria |
| repository.name.fl_str_mv |
|
| repository.mail.fl_str_mv |
|
| _version_ |
1869419352264015872 |
| score |
15.301629 |