Screening great ape museum specimens for DNA viruses

Natural history museum collections harbour a record of wild species from the past centuries, providing a unique opportunity to study animals as well as their infectious agents. Thousands of great ape specimens are kept in these collections, and could become an important resource for studying the evo...

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Autores: Hämmerle, Michelle, Guellil, Meriam, Trgovec-Greif, Lovro, Cheronet, Olivia, Sawyer, Susanna, Ruiz-Gartzia, Irune, Lizano González, Esther, 1974-, Rymbekova, Aigerim, Gelabert Xirinachs, Pere, 1991-, Bernardi, Paolo, Han, Sojung, Rattei, Thomas, Schuenemann, Verena J., Marquès i Bonet, Tomàs, 1975-, Guschanski, Katerina, Calvignac-Spencer, Sebastien, Pinhasi, Ron, Kuhlwilm, Martin
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2024
País:España
Recursos:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:10230/69493
Acesso em linha:http://hdl.handle.net/10230/69493
http://dx.doi.org/10.1038/s41598-024-80780-w
Access Level:acceso abierto
Palavra-chave:Great apes
Hepatitis B virus
Museomics
Target-enrichment capture
Viruses
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dc.title.none.fl_str_mv Screening great ape museum specimens for DNA viruses
title Screening great ape museum specimens for DNA viruses
spellingShingle Screening great ape museum specimens for DNA viruses
Hämmerle, Michelle
Great apes
Hepatitis B virus
Museomics
Target-enrichment capture
Viruses
title_short Screening great ape museum specimens for DNA viruses
title_full Screening great ape museum specimens for DNA viruses
title_fullStr Screening great ape museum specimens for DNA viruses
title_full_unstemmed Screening great ape museum specimens for DNA viruses
title_sort Screening great ape museum specimens for DNA viruses
dc.creator.none.fl_str_mv Hämmerle, Michelle
Guellil, Meriam
Trgovec-Greif, Lovro
Cheronet, Olivia
Sawyer, Susanna
Ruiz-Gartzia, Irune
Lizano González, Esther, 1974-
Rymbekova, Aigerim
Gelabert Xirinachs, Pere, 1991-
Bernardi, Paolo
Han, Sojung
Rattei, Thomas
Schuenemann, Verena J.
Marquès i Bonet, Tomàs, 1975-
Guschanski, Katerina
Calvignac-Spencer, Sebastien
Pinhasi, Ron
Kuhlwilm, Martin
author Hämmerle, Michelle
author_facet Hämmerle, Michelle
Guellil, Meriam
Trgovec-Greif, Lovro
Cheronet, Olivia
Sawyer, Susanna
Ruiz-Gartzia, Irune
Lizano González, Esther, 1974-
Rymbekova, Aigerim
Gelabert Xirinachs, Pere, 1991-
Bernardi, Paolo
Han, Sojung
Rattei, Thomas
Schuenemann, Verena J.
Marquès i Bonet, Tomàs, 1975-
Guschanski, Katerina
Calvignac-Spencer, Sebastien
Pinhasi, Ron
Kuhlwilm, Martin
author_role author
author2 Guellil, Meriam
Trgovec-Greif, Lovro
Cheronet, Olivia
Sawyer, Susanna
Ruiz-Gartzia, Irune
Lizano González, Esther, 1974-
Rymbekova, Aigerim
Gelabert Xirinachs, Pere, 1991-
Bernardi, Paolo
Han, Sojung
Rattei, Thomas
Schuenemann, Verena J.
Marquès i Bonet, Tomàs, 1975-
Guschanski, Katerina
Calvignac-Spencer, Sebastien
Pinhasi, Ron
Kuhlwilm, Martin
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Great apes
Hepatitis B virus
Museomics
Target-enrichment capture
Viruses
topic Great apes
Hepatitis B virus
Museomics
Target-enrichment capture
Viruses
description Natural history museum collections harbour a record of wild species from the past centuries, providing a unique opportunity to study animals as well as their infectious agents. Thousands of great ape specimens are kept in these collections, and could become an important resource for studying the evolution of DNA viruses. Their genetic material is likely to be preserved in dry museum specimens, as reported previously for monkeypox virus genomes from historical orangutan specimens. Here, we screened 209 great ape museum specimens for 99 different DNA viruses, using hybridization capture coupled with short-read high-throughput sequencing. We determined the presence of multiple viruses within this dataset from historical specimens and obtained several near-complete viral genomes. In particular, we report high-coverage (> 18-fold) hepatitis B virus genomes from one gorilla and two chimpanzee individuals, which are phylogenetically placed within clades infecting the respective host species.
publishDate 2024
dc.date.none.fl_str_mv 2024
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10230/69493
http://dx.doi.org/10.1038/s41598-024-80780-w
url http://hdl.handle.net/10230/69493
http://dx.doi.org/10.1038/s41598-024-80780-w
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Sci Rep. 2024 Nov 30;14(1):29806
info:eu-repo/grantAgreement/EC/H2020/955974
info:eu-repo/grantAgreement/EC/H2020/864203
info:eu-repo/grantAgreement/ES/3PE/PID2021-126004NB-100
dc.rights.none.fl_str_mv http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Nature Research
publisher.none.fl_str_mv Nature Research
dc.source.none.fl_str_mv reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
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spelling Screening great ape museum specimens for DNA virusesHämmerle, MichelleGuellil, MeriamTrgovec-Greif, LovroCheronet, OliviaSawyer, SusannaRuiz-Gartzia, IruneLizano González, Esther, 1974-Rymbekova, AigerimGelabert Xirinachs, Pere, 1991-Bernardi, PaoloHan, SojungRattei, ThomasSchuenemann, Verena J.Marquès i Bonet, Tomàs, 1975-Guschanski, KaterinaCalvignac-Spencer, SebastienPinhasi, RonKuhlwilm, MartinGreat apesHepatitis B virusMuseomicsTarget-enrichment captureVirusesNatural history museum collections harbour a record of wild species from the past centuries, providing a unique opportunity to study animals as well as their infectious agents. Thousands of great ape specimens are kept in these collections, and could become an important resource for studying the evolution of DNA viruses. Their genetic material is likely to be preserved in dry museum specimens, as reported previously for monkeypox virus genomes from historical orangutan specimens. Here, we screened 209 great ape museum specimens for 99 different DNA viruses, using hybridization capture coupled with short-read high-throughput sequencing. We determined the presence of multiple viruses within this dataset from historical specimens and obtained several near-complete viral genomes. In particular, we report high-coverage (> 18-fold) hepatitis B virus genomes from one gorilla and two chimpanzee individuals, which are phylogenetically placed within clades infecting the respective host species.This project has been funded by the Vienna Science and Technology Fund (WWTF) [https://doi.org/10.47379/VRG20001] and by the Austrian Science Fund (FWF) [https://doi.org/10.55776/TAI729] to M.K. K.G. received support from the Swedish Research Council (VR) through Grant 2020-03398. L. T.-G. was supported by the European Union’s Horizon 2020 research and innovation program, under the Marie Skłodowska-Curie Actions Innovative Training Networks grant agreement no. 955974 (VIROINF). T.M.-B. is supported by funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (grant agreement No. 864203), Grant PID2021-126004NB-100 funded by MICIU/AEI/ https://doi.org/10.13039/501100011033 and ERDF/EU(MICIIN/FEDER, UE) and Secretaria d’Universitats i Recerca and CERCA Programme del Departament d’Economia i Coneixement de la Generalitat de Catalunya (GRC 2021 SGR 00177). M.G. was supported by the Austrian Science Fund (FWF) [https://doi.org/10.55776/ESP162]. S.H. was supported by the Austrian Science Fund (FWF) [https://doi.org/10.55776/ESP546].Nature Research202520252024info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttp://hdl.handle.net/10230/69493http://dx.doi.org/10.1038/s41598-024-80780-wreponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésSci Rep. 2024 Nov 30;14(1):29806info:eu-repo/grantAgreement/EC/H2020/955974info:eu-repo/grantAgreement/EC/H2020/864203info:eu-repo/grantAgreement/ES/3PE/PID2021-126004NB-100© The Author(s) 2024. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:10230/694932026-05-29T05:05:01Z
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