Dendrimeric peptides can confer protection against foot-and-mouth disease virus in cattle

Foot-and-mouth disease virus (FMDV) causes a highly contagious disease in cloven-hoofed animals. A synthetic vaccine candidate consisting of dendrimeric peptides harbouring two copies of a B-epitope [VP1(136–154)] linked to a T-cell epitope [3A(21–35)] of FMDV confers protection to type O FMDV chall...

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Autores: Soria, Ivana, Quattrocchi, Valeria, Langellotti, Cecilia, Gammella, Mariela, Digiacomo Sebastian, Garcia de la Torre, Beatriz, Andreu Martínez, David, Montoya, Maria, Sobrino, Francisco, Blanco, Esther, Zamorano, Patricia
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2017
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:10230/33213
Acceso en línea:http://hdl.handle.net/10230/33213
http://dx.doi.org/10.1371/journal.pone.0185184
Access Level:acceso abierto
Palabra clave:Foot-and-mouth disease
Dendrimers
Peptides
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spelling Dendrimeric peptides can confer protection against foot-and-mouth disease virus in cattleSoria, IvanaQuattrocchi, ValeriaLangellotti, CeciliaGammella, MarielaDigiacomo SebastianGarcia de la Torre, BeatrizAndreu Martínez, DavidMontoya, MariaSobrino, FranciscoBlanco, EstherZamorano, PatriciaFoot-and-mouth diseaseDendrimersPeptidesFoot-and-mouth disease virus (FMDV) causes a highly contagious disease in cloven-hoofed animals. A synthetic vaccine candidate consisting of dendrimeric peptides harbouring two copies of a B-epitope [VP1(136–154)] linked to a T-cell epitope [3A(21–35)] of FMDV confers protection to type O FMDV challenge in pigs. Herein we show in cattle that novel dendrimeric peptides bearing a T-cell epitope [VP1(21–40] and two or four copies of a B-cell epitope [VP1(135–160)] from type O1 Campos FMDV (termed B2T and B4T, respectively) elicited FMDV specific immune responses to similar levels to a commercial vaccine. Animals were challenged with FMDV and 100% of vaccinated cattle with B2T or B4T were protected to podal generalization. Moreover, bovines immunized with B4T were completely protected (with no clinical signs) against FMDV challenge after three vaccine doses, which was associated with titers of viral neutralizing antibodies in serum higher than those of B2T group (p< 0.05) and levels of opsonic antibodies similar to those of animals immunized with one dose of FMDV commercial vaccine. Bovines vaccinated with both dendrimeric peptides presented high levels of IgG1 anti FMDV in sera and in mucosa. When IgA in nasal secretions was measured, 20% or 40% of the animals in B2T or B4T groups respectively, showed anti-FMDV IgA titers. In addition, B2T and B4T peptides evoked similar consistent T cell responses, being recognized in vitro by lymphocytes from most of the immunized cattle in the proliferation assay, and from all animals in the IFN-γ production assay. Taken together, these results support the potential of dendrimers B2T or B4T in cattle as a highly valuable, cost-effective FMDV candidate vaccine with DIVA potential.Work at INTA was supported by the National Institute of Agricultural Technology (grant number PNSA 1115052) and cooperation agreement INTA-INIA (Argentina-Spain SA 20938). Evaluation of vaccine immunogenicity and protection in cattle. Work at Universitat Pompeu Fabra and CBMSO was supported by MINECO, Spain (grant number AGL2014-52395-C2).Public Library of Science (PLoS)201720172017info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttp://hdl.handle.net/10230/33213http://dx.doi.org/10.1371/journal.pone.0185184reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésPLoS One. 2017 Sep 26;12(9):e0185184info:eu-repo/grantAgreement/ES/1PE/AGL2014-52395-C2© 2017 Soria et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:10230/332132026-05-29T05:05:01Z
dc.title.none.fl_str_mv Dendrimeric peptides can confer protection against foot-and-mouth disease virus in cattle
title Dendrimeric peptides can confer protection against foot-and-mouth disease virus in cattle
spellingShingle Dendrimeric peptides can confer protection against foot-and-mouth disease virus in cattle
Soria, Ivana
Foot-and-mouth disease
Dendrimers
Peptides
title_short Dendrimeric peptides can confer protection against foot-and-mouth disease virus in cattle
title_full Dendrimeric peptides can confer protection against foot-and-mouth disease virus in cattle
title_fullStr Dendrimeric peptides can confer protection against foot-and-mouth disease virus in cattle
title_full_unstemmed Dendrimeric peptides can confer protection against foot-and-mouth disease virus in cattle
title_sort Dendrimeric peptides can confer protection against foot-and-mouth disease virus in cattle
dc.creator.none.fl_str_mv Soria, Ivana
Quattrocchi, Valeria
Langellotti, Cecilia
Gammella, Mariela
Digiacomo Sebastian
Garcia de la Torre, Beatriz
Andreu Martínez, David
Montoya, Maria
Sobrino, Francisco
Blanco, Esther
Zamorano, Patricia
author Soria, Ivana
author_facet Soria, Ivana
Quattrocchi, Valeria
Langellotti, Cecilia
Gammella, Mariela
Digiacomo Sebastian
Garcia de la Torre, Beatriz
Andreu Martínez, David
Montoya, Maria
Sobrino, Francisco
Blanco, Esther
Zamorano, Patricia
author_role author
author2 Quattrocchi, Valeria
Langellotti, Cecilia
Gammella, Mariela
Digiacomo Sebastian
Garcia de la Torre, Beatriz
Andreu Martínez, David
Montoya, Maria
Sobrino, Francisco
Blanco, Esther
Zamorano, Patricia
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Foot-and-mouth disease
Dendrimers
Peptides
topic Foot-and-mouth disease
Dendrimers
Peptides
description Foot-and-mouth disease virus (FMDV) causes a highly contagious disease in cloven-hoofed animals. A synthetic vaccine candidate consisting of dendrimeric peptides harbouring two copies of a B-epitope [VP1(136–154)] linked to a T-cell epitope [3A(21–35)] of FMDV confers protection to type O FMDV challenge in pigs. Herein we show in cattle that novel dendrimeric peptides bearing a T-cell epitope [VP1(21–40] and two or four copies of a B-cell epitope [VP1(135–160)] from type O1 Campos FMDV (termed B2T and B4T, respectively) elicited FMDV specific immune responses to similar levels to a commercial vaccine. Animals were challenged with FMDV and 100% of vaccinated cattle with B2T or B4T were protected to podal generalization. Moreover, bovines immunized with B4T were completely protected (with no clinical signs) against FMDV challenge after three vaccine doses, which was associated with titers of viral neutralizing antibodies in serum higher than those of B2T group (p< 0.05) and levels of opsonic antibodies similar to those of animals immunized with one dose of FMDV commercial vaccine. Bovines vaccinated with both dendrimeric peptides presented high levels of IgG1 anti FMDV in sera and in mucosa. When IgA in nasal secretions was measured, 20% or 40% of the animals in B2T or B4T groups respectively, showed anti-FMDV IgA titers. In addition, B2T and B4T peptides evoked similar consistent T cell responses, being recognized in vitro by lymphocytes from most of the immunized cattle in the proliferation assay, and from all animals in the IFN-γ production assay. Taken together, these results support the potential of dendrimers B2T or B4T in cattle as a highly valuable, cost-effective FMDV candidate vaccine with DIVA potential.
publishDate 2017
dc.date.none.fl_str_mv 2017
2017
2017
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10230/33213
http://dx.doi.org/10.1371/journal.pone.0185184
url http://hdl.handle.net/10230/33213
http://dx.doi.org/10.1371/journal.pone.0185184
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv PLoS One. 2017 Sep 26;12(9):e0185184
info:eu-repo/grantAgreement/ES/1PE/AGL2014-52395-C2
dc.rights.none.fl_str_mv http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library of Science (PLoS)
publisher.none.fl_str_mv Public Library of Science (PLoS)
dc.source.none.fl_str_mv reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
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repository.mail.fl_str_mv
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