Protein Activity of the Fusarium fujikuroi Rhodopsins CarO and OpsA and Their Relation to Fungus–Plant Interaction

Fungi possess diverse photosensory proteins that allow them to perceive different light wavelengths and to adapt to changing light conditions in their environment. The biological and physiological roles of the green light-sensing rhodopsins in fungi are not yet resolved. The rice plant pathogen Fusa...

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Autores: Adam, Alexander, Deimel, Stephan, Pardo Medina, Javier, García Martínez, Jorge, Konte, Tilen, Ávalos Cordero, Francisco Javier, Limón Mirón, María del Carmen
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2018
País:España
Institución:Universidad de Sevilla (US)
Repositorio:idUS. Depósito de Investigación de la Universidad de Sevilla
OAI Identifier:oai:idus.us.es:11441/70425
Acceso en línea:https://hdl.handle.net/11441/70425
https://doi.org/10.3390/ijms19010215
Access Level:acceso abierto
Palabra clave:fungal rhodopsins
CarO
OpsA
Fusarium fujikuroi
Oryza sativa
rice–plant infection
green light perception
indole-3-acetic acid (IAA)
bakanae
patch-clamp
id ES_c92bfd5aada2cb683aa819af2c56d883
oai_identifier_str oai:idus.us.es:11441/70425
network_acronym_str ES
network_name_str España
repository_id_str
spelling Protein Activity of the Fusarium fujikuroi Rhodopsins CarO and OpsA and Their Relation to Fungus–Plant InteractionAdam, AlexanderDeimel, StephanPardo Medina, JavierGarcía Martínez, JorgeKonte, TilenÁvalos Cordero, Francisco JavierLimón Mirón, María del Carmenfungal rhodopsinsCarOOpsAFusarium fujikuroiOryza sativarice–plant infectiongreen light perceptionindole-3-acetic acid (IAA)bakanaepatch-clampFungi possess diverse photosensory proteins that allow them to perceive different light wavelengths and to adapt to changing light conditions in their environment. The biological and physiological roles of the green light-sensing rhodopsins in fungi are not yet resolved. The rice plant pathogen Fusarium fujikuroi exhibits two different rhodopsins, CarO and OpsA. CarO was previously characterized as a light-driven proton pump. We further analyzed the pumping behavior of CarO by patch-clamp experiments. Our data show that CarO pumping activity is strongly augmented in the presence of the plant hormone indole-3-acetic acid and in sodium acetate, in a dose-dependent manner under slightly acidic conditions. By contrast, under these and other tested conditions, the Neurospora rhodopsin (NR)-like rhodopsin OpsA did not exhibit any pump activity. Basic local alignment search tool (BLAST) searches in the genomes of ascomycetes revealed the occurrence of rhodopsin-encoding genes mainly in phyto-associated or phytopathogenic fungi, suggesting a possible correlation of the presence of rhodopsins with fungal ecology. In accordance, rice plants infected with a CarO-deficient F. fujikuroi strain showed more severe bakanae symptoms than the reference strain, indicating a potential role of the CarO rhodopsin in the regulation of plant infection by this fungus.España Ministerio de Ciencia y tecnología BIO20010-15430Junta de Andalucía CTS-6638MDPIGenéticaMinisterio de Ciencia y Tecnología (MCYT). EspañaJunta de Andalucía2018info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttps://hdl.handle.net/11441/70425https://doi.org/10.3390/ijms19010215reponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)InglésInternational Journal of Molecular Sciences, 19 (1), 215-.BIO20010-15430CTS-6638http://dx.doi.org/10.3390/ijms19010215info:eu-repo/semantics/openAccessoai:idus.us.es:11441/704252026-06-17T12:51:07Z
dc.title.none.fl_str_mv Protein Activity of the Fusarium fujikuroi Rhodopsins CarO and OpsA and Their Relation to Fungus–Plant Interaction
title Protein Activity of the Fusarium fujikuroi Rhodopsins CarO and OpsA and Their Relation to Fungus–Plant Interaction
spellingShingle Protein Activity of the Fusarium fujikuroi Rhodopsins CarO and OpsA and Their Relation to Fungus–Plant Interaction
Adam, Alexander
fungal rhodopsins
CarO
OpsA
Fusarium fujikuroi
Oryza sativa
rice–plant infection
green light perception
indole-3-acetic acid (IAA)
bakanae
patch-clamp
title_short Protein Activity of the Fusarium fujikuroi Rhodopsins CarO and OpsA and Their Relation to Fungus–Plant Interaction
title_full Protein Activity of the Fusarium fujikuroi Rhodopsins CarO and OpsA and Their Relation to Fungus–Plant Interaction
title_fullStr Protein Activity of the Fusarium fujikuroi Rhodopsins CarO and OpsA and Their Relation to Fungus–Plant Interaction
title_full_unstemmed Protein Activity of the Fusarium fujikuroi Rhodopsins CarO and OpsA and Their Relation to Fungus–Plant Interaction
title_sort Protein Activity of the Fusarium fujikuroi Rhodopsins CarO and OpsA and Their Relation to Fungus–Plant Interaction
dc.creator.none.fl_str_mv Adam, Alexander
Deimel, Stephan
Pardo Medina, Javier
García Martínez, Jorge
Konte, Tilen
Ávalos Cordero, Francisco Javier
Limón Mirón, María del Carmen
author Adam, Alexander
author_facet Adam, Alexander
Deimel, Stephan
Pardo Medina, Javier
García Martínez, Jorge
Konte, Tilen
Ávalos Cordero, Francisco Javier
Limón Mirón, María del Carmen
author_role author
author2 Deimel, Stephan
Pardo Medina, Javier
García Martínez, Jorge
Konte, Tilen
Ávalos Cordero, Francisco Javier
Limón Mirón, María del Carmen
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Genética
Ministerio de Ciencia y Tecnología (MCYT). España
Junta de Andalucía
dc.subject.none.fl_str_mv fungal rhodopsins
CarO
OpsA
Fusarium fujikuroi
Oryza sativa
rice–plant infection
green light perception
indole-3-acetic acid (IAA)
bakanae
patch-clamp
topic fungal rhodopsins
CarO
OpsA
Fusarium fujikuroi
Oryza sativa
rice–plant infection
green light perception
indole-3-acetic acid (IAA)
bakanae
patch-clamp
description Fungi possess diverse photosensory proteins that allow them to perceive different light wavelengths and to adapt to changing light conditions in their environment. The biological and physiological roles of the green light-sensing rhodopsins in fungi are not yet resolved. The rice plant pathogen Fusarium fujikuroi exhibits two different rhodopsins, CarO and OpsA. CarO was previously characterized as a light-driven proton pump. We further analyzed the pumping behavior of CarO by patch-clamp experiments. Our data show that CarO pumping activity is strongly augmented in the presence of the plant hormone indole-3-acetic acid and in sodium acetate, in a dose-dependent manner under slightly acidic conditions. By contrast, under these and other tested conditions, the Neurospora rhodopsin (NR)-like rhodopsin OpsA did not exhibit any pump activity. Basic local alignment search tool (BLAST) searches in the genomes of ascomycetes revealed the occurrence of rhodopsin-encoding genes mainly in phyto-associated or phytopathogenic fungi, suggesting a possible correlation of the presence of rhodopsins with fungal ecology. In accordance, rice plants infected with a CarO-deficient F. fujikuroi strain showed more severe bakanae symptoms than the reference strain, indicating a potential role of the CarO rhodopsin in the regulation of plant infection by this fungus.
publishDate 2018
dc.date.none.fl_str_mv 2018
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/11441/70425
https://doi.org/10.3390/ijms19010215
url https://hdl.handle.net/11441/70425
https://doi.org/10.3390/ijms19010215
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv International Journal of Molecular Sciences, 19 (1), 215-.
BIO20010-15430
CTS-6638
http://dx.doi.org/10.3390/ijms19010215
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:idUS. Depósito de Investigación de la Universidad de Sevilla
instname:Universidad de Sevilla (US)
instname_str Universidad de Sevilla (US)
reponame_str idUS. Depósito de Investigación de la Universidad de Sevilla
collection idUS. Depósito de Investigación de la Universidad de Sevilla
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300719

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