Bicistronic CAR-T Cell against BCMA and CD229 effectively controls myeloma even when BCMA expression is limited.

Anti-BCMA CAR-T cell therapy has revolutionized the prognosis of relapsed / refractory multiple myeloma patients. Regrettably, despite unprecedented overall response rates achieved with this approach, most patients eventually relapse. One of the primary reasons for this is the complete loss or reduc...

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Detalles Bibliográficos
Autores: Rodríguez Lobato, Luis Gerardo, Cardús Granell, Oriol, Mañé Pujol, Joan, Battram, Anthony M., Vaqué Salsench, Sergi, Carpio Marmol, Judit, Pérez Amill, Lorena, Calderón, Hugo, Martín Antonio, Araceli Beatriz, Oliver Caldés, Aina, Lozano Garcia, Ester, Moreno Fajardo, David Fernando, Ortiz Maldonado, Valentín, Salas Gay, María Queralt, Daniel Bisbe, Anna de, Tovar Gomis, Natalia, Cibeira López, M. Teresa, Rosiñol Dachs, Laura, Bladé, J. (Joan), Juan, Manel, Urbano Ispizua, Álvaro, Engel Rocamora, Pablo, Fernández de Larrea Rodríguez, Carlos José
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2025
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/223052
Acceso en línea:https://hdl.handle.net/2445/223052
Access Level:acceso embargado
Palabra clave:Mieloma múltiple
Teràpia cel·lular
Multiple myeloma
T cells
Descripción
Sumario:Anti-BCMA CAR-T cell therapy has revolutionized the prognosis of relapsed / refractory multiple myeloma patients. Regrettably, despite unprecedented overall response rates achieved with this approach, most patients eventually relapse. One of the primary reasons for this is the complete loss or reduced expression of BCMA on the malignant plasma cell surface. Consequently, new therapeutic targets are under investigation. Another potential therapeutic approach involves the use of CAR-T cells targeting two tumor antigens. In this study, we developed and validated a monospecific CAR targeting CD229, which was effective in in vitro and in vivo NSG mouse models with both homogeneous and heterogeneous BCMA expression. Additionally, we created a bicistronic CAR-T cell targeting both CD229 and BCMA, which demonstrated efficacy in models with homogeneous BCMA expression, in heterogeneous models featuring small clonal populations with biallelic BCMA deletion, and in cases with reduced BCMA expression both in vivo and in vitro. Regarding "on-target off-tumor toxicity," no fratricide was observed among CAR-T cells, but there was a limited elimination of non-activated T-cells. The immune pressure exerted by anti-CD229 CAR-T cells led to the loss of the CD229 antigen expression in some instances. In summary, this work underscores the potential utility of CD229 alone or in combination with BCMA, as an immunotherapeutic target in multiple myeloma, especially in cases marked by diminished or absent BCMA expression.

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