The pluripotency factor NANOG controls primitive hematopoiesis and directly regulates Tal1

Includes supplementary materials: online appendix; replication data

Detalhes bibliográficos
Autores: Sainz de Aja, Julio, Menchero, Sergio, Rollan, Isabel, Barral, Antonio, Tiana, Maria, Jawaid, Wajid, Cossio, Itziar, Alvarez, Alba, Carreño-Tarragona, Gonzalo, Badia-Careaga, Claudio, Nichols, Jennifer, Göttgens, Berthold, Isern, Joan, Manzanares, Miguel
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:España
Recursos:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:10230/55614
Acesso em linha:http://hdl.handle.net/10230/55614
http://dx.doi.org/10.15252/embj.201899122
Access Level:acceso abierto
Palavra-chave:Erythropoiesis
Gastrulation
Nanog
Primitive hematopoiesis
Tal1
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spelling The pluripotency factor NANOG controls primitive hematopoiesis and directly regulates Tal1Sainz de Aja, JulioMenchero, SergioRollan, IsabelBarral, AntonioTiana, MariaJawaid, WajidCossio, ItziarAlvarez, AlbaCarreño-Tarragona, GonzaloBadia-Careaga, ClaudioNichols, JenniferGöttgens, BertholdIsern, JoanManzanares, MiguelErythropoiesisGastrulationNanogPrimitive hematopoiesisTal1Includes supplementary materials: online appendix; replication dataProgenitors of the first hematopoietic cells in the mouse arise in the early embryo from Brachyury-positive multipotent cells in the posterior-proximal region of the epiblast, but the mechanisms that specify primitive blood cells are still largely unknown. Pluripotency factors maintain uncommitted cells of the blastocyst and embryonic stem cells in the pluripotent state. However, little is known about the role played by these factors during later development, despite being expressed in the postimplantation epiblast. Using a dual transgene system for controlled expression at postimplantation stages, we found that Nanog blocks primitive hematopoiesis in the gastrulating embryo, resulting in a loss of red blood cells and downregulation of erythropoietic genes. Accordingly, Nanog-deficient embryonic stem cells are prone to erythropoietic differentiation. Moreover, Nanog expression in adults prevents the maturation of erythroid cells. By analysis of previous data for NANOG binding during stem cell differentiation and CRISPR/Cas9 genome editing, we found that Tal1 is a direct NANOG target. Our results show that Nanog regulates primitive hematopoiesis by directly repressing critical erythroid lineage specifiers.This work was supported by the Spanish government (grant BFU2014-54608-P and BFU2017-84914-P to MM; grants RYC-2011-09209 and BFU-2012-35892 to JI). The Gottgens and Nichols laboratories are supported by core funding from the Wellcome Trust and MRC to the Wellcome and MRC Cambridge Stem Cell Institute. The CNIC is supported by the Spanish Ministry of Science, Innovation and Universities (MINECO) and the Pro CNIC Foundation, and is a Severo Ochoa Center of Excellence (SEV-2015-0505).EMBO Press202320232019info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/10230/55614http://dx.doi.org/10.15252/embj.201899122reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésThe EMBO Journal. 2019 Apr 01;38(7):e99122info:eu-repo/grantAgreement/ES/1PE/BFU2014-54608-Pinfo:eu-repo/grantAgreement/ES/2PE/BFU2017-84914-Pinfo:eu-repo/grantAgreement/ES/3PN/BFU2012-35892This is an open access article under the terms of the Creative Commons Attribution 4.0 License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:10230/556142026-05-29T05:05:01Z
dc.title.none.fl_str_mv The pluripotency factor NANOG controls primitive hematopoiesis and directly regulates Tal1
title The pluripotency factor NANOG controls primitive hematopoiesis and directly regulates Tal1
spellingShingle The pluripotency factor NANOG controls primitive hematopoiesis and directly regulates Tal1
Sainz de Aja, Julio
Erythropoiesis
Gastrulation
Nanog
Primitive hematopoiesis
Tal1
title_short The pluripotency factor NANOG controls primitive hematopoiesis and directly regulates Tal1
title_full The pluripotency factor NANOG controls primitive hematopoiesis and directly regulates Tal1
title_fullStr The pluripotency factor NANOG controls primitive hematopoiesis and directly regulates Tal1
title_full_unstemmed The pluripotency factor NANOG controls primitive hematopoiesis and directly regulates Tal1
title_sort The pluripotency factor NANOG controls primitive hematopoiesis and directly regulates Tal1
dc.creator.none.fl_str_mv Sainz de Aja, Julio
Menchero, Sergio
Rollan, Isabel
Barral, Antonio
Tiana, Maria
Jawaid, Wajid
Cossio, Itziar
Alvarez, Alba
Carreño-Tarragona, Gonzalo
Badia-Careaga, Claudio
Nichols, Jennifer
Göttgens, Berthold
Isern, Joan
Manzanares, Miguel
author Sainz de Aja, Julio
author_facet Sainz de Aja, Julio
Menchero, Sergio
Rollan, Isabel
Barral, Antonio
Tiana, Maria
Jawaid, Wajid
Cossio, Itziar
Alvarez, Alba
Carreño-Tarragona, Gonzalo
Badia-Careaga, Claudio
Nichols, Jennifer
Göttgens, Berthold
Isern, Joan
Manzanares, Miguel
author_role author
author2 Menchero, Sergio
Rollan, Isabel
Barral, Antonio
Tiana, Maria
Jawaid, Wajid
Cossio, Itziar
Alvarez, Alba
Carreño-Tarragona, Gonzalo
Badia-Careaga, Claudio
Nichols, Jennifer
Göttgens, Berthold
Isern, Joan
Manzanares, Miguel
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Erythropoiesis
Gastrulation
Nanog
Primitive hematopoiesis
Tal1
topic Erythropoiesis
Gastrulation
Nanog
Primitive hematopoiesis
Tal1
description Includes supplementary materials: online appendix; replication data
publishDate 2019
dc.date.none.fl_str_mv 2019
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10230/55614
http://dx.doi.org/10.15252/embj.201899122
url http://hdl.handle.net/10230/55614
http://dx.doi.org/10.15252/embj.201899122
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv The EMBO Journal. 2019 Apr 01;38(7):e99122
info:eu-repo/grantAgreement/ES/1PE/BFU2014-54608-P
info:eu-repo/grantAgreement/ES/2PE/BFU2017-84914-P
info:eu-repo/grantAgreement/ES/3PN/BFU2012-35892
dc.rights.none.fl_str_mv http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv EMBO Press
publisher.none.fl_str_mv EMBO Press
dc.source.none.fl_str_mv reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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