KatE from the bacterial plant pathogen ralstonia solanacearumis a monofunctional catalase controlled by HrpG that plays a major role in bacterial survival to hydrogen peroxide

Ralstonia solanacearum is the causative agent of bacterial wilt disease on a wide range of plant species. Besides the numerous bacterial activities required for host invasion, those involved in the adaptation to the plant environment are key for the success of infection. R. solanacearum ability to c...

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Autores: Tondo, Maria Laura, Pedro Jové, Roger de, Vandecaveye, Agustina, Piskulic, Laura, Orellano, Elena G., Valls i Matheu, Marc
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/175085
Acceso en línea:https://hdl.handle.net/2445/175085
Access Level:acceso abierto
Palabra clave:Bacteris patògens
Peròxids
Plantes
Pathogenic bacteria
Peroxides
Plants
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spelling KatE from the bacterial plant pathogen ralstonia solanacearumis a monofunctional catalase controlled by HrpG that plays a major role in bacterial survival to hydrogen peroxideTondo, Maria LauraPedro Jové, Roger deVandecaveye, AgustinaPiskulic, LauraOrellano, Elena G.Valls i Matheu, MarcBacteris patògensPeròxidsPlantesPathogenic bacteriaPeroxidesPlantsRalstonia solanacearum is the causative agent of bacterial wilt disease on a wide range of plant species. Besides the numerous bacterial activities required for host invasion, those involved in the adaptation to the plant environment are key for the success of infection. R. solanacearum ability to cope with the oxidative burst produced by the plant is likely one of the activities required to grow parasitically. Among the multiple reactive oxygen species (ROS)-scavenging enzymes predicted in the R. solanacearum GMI1000 genome, a single monofunctional catalase (KatE) and two KatG bifunctional catalases were identified. In this work, we show that these catalase activities are active in bacterial protein extracts and demonstrate by gene disruption and mutant complementation that the monofunctional catalase activity is encoded by katE. Different strategies were used to evaluate the role of KatE in bacterial physiology and during the infection process that causes bacterial wilt. We show that the activity of the enzyme is maximal during exponential growth in vitro and this growth-phase regulation occurs at the transcriptional level. Our studies also demonstrate that katE expression is transcriptionally activated by HrpG, a central regulator of R. solanacearum induced upon contact with the plant cells. In addition, we reveal that even though both KatE and KatG catalase activities are induced upon hydrogen peroxide treatment, KatE has a major effect on bacterial survival under oxidative stress conditions and especially in the adaptive response of R. solanacearum to this oxidant. The katE mutant strain also exhibited differences in the structural characteristics of the biofilms developed on an abiotic surface in comparison to wild-type cells, but not in the overall amount of biofilm production. The role of catalase KatE during the interaction with its host plant tomato is also studied, revealing that disruption of this gene has no effect on R. solanacearum virulence or bacterial growth in leave tissues, which suggests a minor role for this catalase in bacterial fitness in planta. Our work provides the first characterization of the R. solanacearum catalases and identifies KatE as a bona fide monofunctional catalase with an important role in bacterial protection against oxidative stress.Frontiers Media2020info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/175085Articles publicats en revistes (Genètica, Microbiologia i Estadística)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.3389/fpls.2020.01156Frontiers in Plant Science, 2020, vol. 11https://doi.org/10.3389/fpls.2020.01156cc-by (c) Tondo, Maria Laura et al., 2020http://creativecommons.org/licenses/by/3.0/esinfo:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1750852026-05-27T06:46:51Z
dc.title.none.fl_str_mv KatE from the bacterial plant pathogen ralstonia solanacearumis a monofunctional catalase controlled by HrpG that plays a major role in bacterial survival to hydrogen peroxide
title KatE from the bacterial plant pathogen ralstonia solanacearumis a monofunctional catalase controlled by HrpG that plays a major role in bacterial survival to hydrogen peroxide
spellingShingle KatE from the bacterial plant pathogen ralstonia solanacearumis a monofunctional catalase controlled by HrpG that plays a major role in bacterial survival to hydrogen peroxide
Tondo, Maria Laura
Bacteris patògens
Peròxids
Plantes
Pathogenic bacteria
Peroxides
Plants
title_short KatE from the bacterial plant pathogen ralstonia solanacearumis a monofunctional catalase controlled by HrpG that plays a major role in bacterial survival to hydrogen peroxide
title_full KatE from the bacterial plant pathogen ralstonia solanacearumis a monofunctional catalase controlled by HrpG that plays a major role in bacterial survival to hydrogen peroxide
title_fullStr KatE from the bacterial plant pathogen ralstonia solanacearumis a monofunctional catalase controlled by HrpG that plays a major role in bacterial survival to hydrogen peroxide
title_full_unstemmed KatE from the bacterial plant pathogen ralstonia solanacearumis a monofunctional catalase controlled by HrpG that plays a major role in bacterial survival to hydrogen peroxide
title_sort KatE from the bacterial plant pathogen ralstonia solanacearumis a monofunctional catalase controlled by HrpG that plays a major role in bacterial survival to hydrogen peroxide
dc.creator.none.fl_str_mv Tondo, Maria Laura
Pedro Jové, Roger de
Vandecaveye, Agustina
Piskulic, Laura
Orellano, Elena G.
Valls i Matheu, Marc
author Tondo, Maria Laura
author_facet Tondo, Maria Laura
Pedro Jové, Roger de
Vandecaveye, Agustina
Piskulic, Laura
Orellano, Elena G.
Valls i Matheu, Marc
author_role author
author2 Pedro Jové, Roger de
Vandecaveye, Agustina
Piskulic, Laura
Orellano, Elena G.
Valls i Matheu, Marc
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Bacteris patògens
Peròxids
Plantes
Pathogenic bacteria
Peroxides
Plants
topic Bacteris patògens
Peròxids
Plantes
Pathogenic bacteria
Peroxides
Plants
description Ralstonia solanacearum is the causative agent of bacterial wilt disease on a wide range of plant species. Besides the numerous bacterial activities required for host invasion, those involved in the adaptation to the plant environment are key for the success of infection. R. solanacearum ability to cope with the oxidative burst produced by the plant is likely one of the activities required to grow parasitically. Among the multiple reactive oxygen species (ROS)-scavenging enzymes predicted in the R. solanacearum GMI1000 genome, a single monofunctional catalase (KatE) and two KatG bifunctional catalases were identified. In this work, we show that these catalase activities are active in bacterial protein extracts and demonstrate by gene disruption and mutant complementation that the monofunctional catalase activity is encoded by katE. Different strategies were used to evaluate the role of KatE in bacterial physiology and during the infection process that causes bacterial wilt. We show that the activity of the enzyme is maximal during exponential growth in vitro and this growth-phase regulation occurs at the transcriptional level. Our studies also demonstrate that katE expression is transcriptionally activated by HrpG, a central regulator of R. solanacearum induced upon contact with the plant cells. In addition, we reveal that even though both KatE and KatG catalase activities are induced upon hydrogen peroxide treatment, KatE has a major effect on bacterial survival under oxidative stress conditions and especially in the adaptive response of R. solanacearum to this oxidant. The katE mutant strain also exhibited differences in the structural characteristics of the biofilms developed on an abiotic surface in comparison to wild-type cells, but not in the overall amount of biofilm production. The role of catalase KatE during the interaction with its host plant tomato is also studied, revealing that disruption of this gene has no effect on R. solanacearum virulence or bacterial growth in leave tissues, which suggests a minor role for this catalase in bacterial fitness in planta. Our work provides the first characterization of the R. solanacearum catalases and identifies KatE as a bona fide monofunctional catalase with an important role in bacterial protection against oxidative stress.
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/175085
url https://hdl.handle.net/2445/175085
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.3389/fpls.2020.01156
Frontiers in Plant Science, 2020, vol. 11
https://doi.org/10.3389/fpls.2020.01156
dc.rights.none.fl_str_mv cc-by (c) Tondo, Maria Laura et al., 2020
http://creativecommons.org/licenses/by/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Tondo, Maria Laura et al., 2020
http://creativecommons.org/licenses/by/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv Articles publicats en revistes (Genètica, Microbiologia i Estadística)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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