Priming human adipose-derived mesenchymal stem cells for corneal surface regeneration.

Limbal stem cells (LSC) maintain the transparency of the corneal epithelium. Chemical burns lead the loss of LSC inducing an up-regulation of pro-inflammatory and pro-angiogenic factors, triggering corneal neovascularization and blindness. Adipose tissue-derived mesenchymal stem cells (AT-MSC) have...

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Autores: Nieto Nicolau, Núria, Martínez Conesa, Eva M., Fuentes Julián, Sherezade, Arnalich Montiel, Francisco, García Tuñón, Ignacio, De Miguel, María P., Casaroli Marano, Ricardo Pedro
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/183499
Acceso en línea:https://hdl.handle.net/2445/183499
Access Level:acceso abierto
Palabra clave:Cèl·lules mare
Còrnia
Cultiu cel·lular
Stem cells
Cornea
Cell culture
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spelling Priming human adipose-derived mesenchymal stem cells for corneal surface regeneration.Nieto Nicolau, NúriaMartínez Conesa, Eva M.Fuentes Julián, SherezadeArnalich Montiel, FranciscoGarcía Tuñón, IgnacioDe Miguel, María P.Casaroli Marano, Ricardo PedroCèl·lules mareCòrniaCultiu cel·lularStem cellsCorneaCell cultureLimbal stem cells (LSC) maintain the transparency of the corneal epithelium. Chemical burns lead the loss of LSC inducing an up-regulation of pro-inflammatory and pro-angiogenic factors, triggering corneal neovascularization and blindness. Adipose tissue-derived mesenchymal stem cells (AT-MSC) have shown promise in animal models to treat LSC deficiency (LSCD), but there are not studies showing their efficacy when primed with different media before transplantation. We cultured AT-MSC with standard medium and media used to culture LSC for clinical application. We demonstrated that different media changed the AT-MSC paracrine secretion showing different paracrine effector functions in an in vivo model of chemical burn and in response to a novel in vitro model of corneal inflammation by alkali induction. Treatment of LSCD with AT-MSC changed the angiogenic and inflammatory cytokine profile of mice corneas. AT-MSC cultured with the medium that improved their cytokine secretion, enhanced the anti-angiogenic and anti-inflammatory profile of the treated corneas. Those corneas also presented better outcome in terms of corneal transparency, neovascularization and histologic reconstruction. Priming human AT-MSC with LSC specific medium can potentiate their ability to improve corneal wound healing, decrease neovascularization and inflammation modulating paracrine effector functions in an in vivo optimized rat model of LSCD.John Wiley & Sons2022202220212022info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion14 p.application/pdfhttps://hdl.handle.net/2445/183499Articles publicats en revistes (Cirurgia i Especialitats Medicoquirúrgiques)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: https://doi.org/10.1111/jcmm.16501Journal of Cellular and Molecular Medicine, 2021, vol. 25, num. 11, p. 5124-5137https://doi.org/10.1111/jcmm.16501cc-by (c) Nieto Nicolau, Núria et al., 2021https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:2445/1834992026-05-29T05:05:01Z
dc.title.none.fl_str_mv Priming human adipose-derived mesenchymal stem cells for corneal surface regeneration.
title Priming human adipose-derived mesenchymal stem cells for corneal surface regeneration.
spellingShingle Priming human adipose-derived mesenchymal stem cells for corneal surface regeneration.
Nieto Nicolau, Núria
Cèl·lules mare
Còrnia
Cultiu cel·lular
Stem cells
Cornea
Cell culture
title_short Priming human adipose-derived mesenchymal stem cells for corneal surface regeneration.
title_full Priming human adipose-derived mesenchymal stem cells for corneal surface regeneration.
title_fullStr Priming human adipose-derived mesenchymal stem cells for corneal surface regeneration.
title_full_unstemmed Priming human adipose-derived mesenchymal stem cells for corneal surface regeneration.
title_sort Priming human adipose-derived mesenchymal stem cells for corneal surface regeneration.
dc.creator.none.fl_str_mv Nieto Nicolau, Núria
Martínez Conesa, Eva M.
Fuentes Julián, Sherezade
Arnalich Montiel, Francisco
García Tuñón, Ignacio
De Miguel, María P.
Casaroli Marano, Ricardo Pedro
author Nieto Nicolau, Núria
author_facet Nieto Nicolau, Núria
Martínez Conesa, Eva M.
Fuentes Julián, Sherezade
Arnalich Montiel, Francisco
García Tuñón, Ignacio
De Miguel, María P.
Casaroli Marano, Ricardo Pedro
author_role author
author2 Martínez Conesa, Eva M.
Fuentes Julián, Sherezade
Arnalich Montiel, Francisco
García Tuñón, Ignacio
De Miguel, María P.
Casaroli Marano, Ricardo Pedro
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Cèl·lules mare
Còrnia
Cultiu cel·lular
Stem cells
Cornea
Cell culture
topic Cèl·lules mare
Còrnia
Cultiu cel·lular
Stem cells
Cornea
Cell culture
description Limbal stem cells (LSC) maintain the transparency of the corneal epithelium. Chemical burns lead the loss of LSC inducing an up-regulation of pro-inflammatory and pro-angiogenic factors, triggering corneal neovascularization and blindness. Adipose tissue-derived mesenchymal stem cells (AT-MSC) have shown promise in animal models to treat LSC deficiency (LSCD), but there are not studies showing their efficacy when primed with different media before transplantation. We cultured AT-MSC with standard medium and media used to culture LSC for clinical application. We demonstrated that different media changed the AT-MSC paracrine secretion showing different paracrine effector functions in an in vivo model of chemical burn and in response to a novel in vitro model of corneal inflammation by alkali induction. Treatment of LSCD with AT-MSC changed the angiogenic and inflammatory cytokine profile of mice corneas. AT-MSC cultured with the medium that improved their cytokine secretion, enhanced the anti-angiogenic and anti-inflammatory profile of the treated corneas. Those corneas also presented better outcome in terms of corneal transparency, neovascularization and histologic reconstruction. Priming human AT-MSC with LSC specific medium can potentiate their ability to improve corneal wound healing, decrease neovascularization and inflammation modulating paracrine effector functions in an in vivo optimized rat model of LSCD.
publishDate 2021
dc.date.none.fl_str_mv 2021
2022
2022
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/183499
url https://hdl.handle.net/2445/183499
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1111/jcmm.16501
Journal of Cellular and Molecular Medicine, 2021, vol. 25, num. 11, p. 5124-5137
https://doi.org/10.1111/jcmm.16501
dc.rights.none.fl_str_mv cc-by (c) Nieto Nicolau, Núria et al., 2021
https://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Nieto Nicolau, Núria et al., 2021
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 14 p.
application/pdf
dc.publisher.none.fl_str_mv John Wiley & Sons
publisher.none.fl_str_mv John Wiley & Sons
dc.source.none.fl_str_mv Articles publicats en revistes (Cirurgia i Especialitats Medicoquirúrgiques)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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