Combining confocal microscopy, dSTORM, and mass spectroscopy to unveil the evolution of the protein corona associated with nanostructured lipid carriers during blood-brain barrier crossing

Upon coming into contact with the biological environment, nanostructures are immediately covered by biomolecules, particularly by proteins forming the so-called "protein corona" (PC). The phenomenon of PC formation has gained great attention in recent years due to its implication in the us...

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Authors: Battaglini, Matteo, Feiner Gracia, Natalia, Tapeinos, Christos, Pasquale, Daniele de, Pucci, Carlotta, Marino, Attilio, Bartolucci, Martina, Petretto, Andrea, Albertazzi, Lorenzo, Ciofani, Gianni
Format: article
Status:Published version
Publication Date:2022
Country:España
Institution:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repository:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/190605
Online Access:https://hdl.handle.net/2445/190605
Access Level:Open access
Keyword:Microscòpia confocal
Proteòmica
Confocal microscopy
Proteomics
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spelling Combining confocal microscopy, dSTORM, and mass spectroscopy to unveil the evolution of the protein corona associated with nanostructured lipid carriers during blood-brain barrier crossingBattaglini, MatteoFeiner Gracia, NataliaTapeinos, ChristosPasquale, Daniele dePucci, CarlottaMarino, AttilioBartolucci, MartinaPetretto, AndreaAlbertazzi, LorenzoCiofani, GianniMicroscòpia confocalProteòmicaConfocal microscopyProteomicsUpon coming into contact with the biological environment, nanostructures are immediately covered by biomolecules, particularly by proteins forming the so-called "protein corona" (PC). The phenomenon of PC formation has gained great attention in recent years due to its implication in the use of nanostructures in biomedicine. In fact, it has been shown that the formation of the PC can impact the performance of nanostructures by reducing their stability, causing aggregation, increasing their toxicity, and providing unexpected and undesired nanostructure-cell interactions. In this work, we decided to study for the first time the formation and the evolution of PC on the surface of nanostructured lipid carriers loaded with superparamagnetic iron oxide nanoparticles, before and after the crossing of an in vitro model of the blood-brain barrier (BBB). Combining confocal microscopy, direct STochastic Optical Reconstruction Microscopy (dSTORM), and proteomic analysis, we were able to carry out a complete analysis of the PC formation and evolution. In particular, we highlighted that PC formation is a fast process, being formed around particles even after just 1 min of exposure to fetal bovine serum. Moreover, PC formed around particles is extremely heterogeneous: while some particles have no associated PC at all, others are completely covered by proteins. Lastly, the interaction with an in vitro BBB model strongly affects the PC composition: in particular, a large amount of the proteins forming the initial PC is lost after the BBB passage and they are partially replaced by new proteins derived from both the brain endothelial cells and the cell culture medium. Altogether, the obtained data could potentially provide new insights into the design and fabrication of lipid nanostructures for the treatment of central nervous system disorders.2022202220222022info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion16 p.application/pdfhttps://hdl.handle.net/2445/190605Articles publicats en revistes (Institut de Bioenginyeria de Catalunya (IBEC))reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: https://doi.org/10.1039/d2nr00484dNanoscale, 2022, vol. 14, num. 36, p. 13292-13307https://doi.org/10.1039/d2nr00484dinfo:eu-repo/grantAgreement/EC/H2020/709613cc by-nc (c) Battaglini, Matteo et al, 2022http://creativecommons.org/licenses/by-nc/3.0/es/info:eu-repo/semantics/openAccessoai:recercat.cat:2445/1906052026-05-29T05:05:01Z
dc.title.none.fl_str_mv Combining confocal microscopy, dSTORM, and mass spectroscopy to unveil the evolution of the protein corona associated with nanostructured lipid carriers during blood-brain barrier crossing
title Combining confocal microscopy, dSTORM, and mass spectroscopy to unveil the evolution of the protein corona associated with nanostructured lipid carriers during blood-brain barrier crossing
spellingShingle Combining confocal microscopy, dSTORM, and mass spectroscopy to unveil the evolution of the protein corona associated with nanostructured lipid carriers during blood-brain barrier crossing
Battaglini, Matteo
Microscòpia confocal
Proteòmica
Confocal microscopy
Proteomics
title_short Combining confocal microscopy, dSTORM, and mass spectroscopy to unveil the evolution of the protein corona associated with nanostructured lipid carriers during blood-brain barrier crossing
title_full Combining confocal microscopy, dSTORM, and mass spectroscopy to unveil the evolution of the protein corona associated with nanostructured lipid carriers during blood-brain barrier crossing
title_fullStr Combining confocal microscopy, dSTORM, and mass spectroscopy to unveil the evolution of the protein corona associated with nanostructured lipid carriers during blood-brain barrier crossing
title_full_unstemmed Combining confocal microscopy, dSTORM, and mass spectroscopy to unveil the evolution of the protein corona associated with nanostructured lipid carriers during blood-brain barrier crossing
title_sort Combining confocal microscopy, dSTORM, and mass spectroscopy to unveil the evolution of the protein corona associated with nanostructured lipid carriers during blood-brain barrier crossing
dc.creator.none.fl_str_mv Battaglini, Matteo
Feiner Gracia, Natalia
Tapeinos, Christos
Pasquale, Daniele de
Pucci, Carlotta
Marino, Attilio
Bartolucci, Martina
Petretto, Andrea
Albertazzi, Lorenzo
Ciofani, Gianni
author Battaglini, Matteo
author_facet Battaglini, Matteo
Feiner Gracia, Natalia
Tapeinos, Christos
Pasquale, Daniele de
Pucci, Carlotta
Marino, Attilio
Bartolucci, Martina
Petretto, Andrea
Albertazzi, Lorenzo
Ciofani, Gianni
author_role author
author2 Feiner Gracia, Natalia
Tapeinos, Christos
Pasquale, Daniele de
Pucci, Carlotta
Marino, Attilio
Bartolucci, Martina
Petretto, Andrea
Albertazzi, Lorenzo
Ciofani, Gianni
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Microscòpia confocal
Proteòmica
Confocal microscopy
Proteomics
topic Microscòpia confocal
Proteòmica
Confocal microscopy
Proteomics
description Upon coming into contact with the biological environment, nanostructures are immediately covered by biomolecules, particularly by proteins forming the so-called "protein corona" (PC). The phenomenon of PC formation has gained great attention in recent years due to its implication in the use of nanostructures in biomedicine. In fact, it has been shown that the formation of the PC can impact the performance of nanostructures by reducing their stability, causing aggregation, increasing their toxicity, and providing unexpected and undesired nanostructure-cell interactions. In this work, we decided to study for the first time the formation and the evolution of PC on the surface of nanostructured lipid carriers loaded with superparamagnetic iron oxide nanoparticles, before and after the crossing of an in vitro model of the blood-brain barrier (BBB). Combining confocal microscopy, direct STochastic Optical Reconstruction Microscopy (dSTORM), and proteomic analysis, we were able to carry out a complete analysis of the PC formation and evolution. In particular, we highlighted that PC formation is a fast process, being formed around particles even after just 1 min of exposure to fetal bovine serum. Moreover, PC formed around particles is extremely heterogeneous: while some particles have no associated PC at all, others are completely covered by proteins. Lastly, the interaction with an in vitro BBB model strongly affects the PC composition: in particular, a large amount of the proteins forming the initial PC is lost after the BBB passage and they are partially replaced by new proteins derived from both the brain endothelial cells and the cell culture medium. Altogether, the obtained data could potentially provide new insights into the design and fabrication of lipid nanostructures for the treatment of central nervous system disorders.
publishDate 2022
dc.date.none.fl_str_mv 2022
2022
2022
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/190605
url https://hdl.handle.net/2445/190605
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1039/d2nr00484d
Nanoscale, 2022, vol. 14, num. 36, p. 13292-13307
https://doi.org/10.1039/d2nr00484d
info:eu-repo/grantAgreement/EC/H2020/709613
dc.rights.none.fl_str_mv cc by-nc (c) Battaglini, Matteo et al, 2022
http://creativecommons.org/licenses/by-nc/3.0/es/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc by-nc (c) Battaglini, Matteo et al, 2022
http://creativecommons.org/licenses/by-nc/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 16 p.
application/pdf
dc.source.none.fl_str_mv Articles publicats en revistes (Institut de Bioenginyeria de Catalunya (IBEC))
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.811543

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