CD200R1 Contributes to Successful Functional Reinnervation after a Sciatic Nerve Injury

Activating and inhibitory immune receptors play a critical role in regulating systemic and central nervous system (CNS) immune and inflammatory processes. The CD200R1 immunoreceptor induces a restraining signal modulating inflammation and phagocytosis in the CNS under different inflammatory conditio...

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Autores: Pannunzio, Bruno, Amo Aparicio, Jesús|||0000-0002-1294-6819, Julián, Camila, López Vales, Rubén|||0000-0001-7615-9550, Peluffo, Hugo|||0000-0001-6111-8668, Lago, Natalia|||0000-0002-5631-8558
Tipo de recurso: artículo
Fecha de publicación:2022
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:259846
Acceso en línea:https://ddd.uab.cat/record/259846
https://dx.doi.org/urn:doi:10.3390/cells11111786
Access Level:acceso abierto
Palabra clave:CD200R1 blocking antibody
Immune receptor
Macrophages
Regeneration
Functional reinnervation
Wallerian Degeneration
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spelling CD200R1 Contributes to Successful Functional Reinnervation after a Sciatic Nerve InjuryPannunzio, BrunoAmo Aparicio, Jesús|||0000-0002-1294-6819Julián, CamilaLópez Vales, Rubén|||0000-0001-7615-9550Peluffo, Hugo|||0000-0001-6111-8668Lago, Natalia|||0000-0002-5631-8558CD200R1 blocking antibodyImmune receptorMacrophagesRegenerationFunctional reinnervationWallerian DegenerationActivating and inhibitory immune receptors play a critical role in regulating systemic and central nervous system (CNS) immune and inflammatory processes. The CD200R1 immunoreceptor induces a restraining signal modulating inflammation and phagocytosis in the CNS under different inflammatory conditions. However, it remains unknown whether CD200R1 has a role in modulating the inflammatory response after a peripheral nerve injury, an essential component of the successful regeneration. Expression of CD200R1 and its ligand CD200 was analyzed during homeostasis and after a sciatic nerve crush injury in C57Bl/6 mice. The role of CD200R1 in Wallerian Degeneration (WD) and nerve regeneration was studied using a specific antibody against CD200R1 injected into the nerve at the time of injury. We found an upregulation of CD200R1 mRNA after injury whereas CD200 was downregulated acutely after nerve injury. Blockade of CD200R1 significantly reduced the acute entrance of both neutrophils and monocytes from blood after nerve injury. When long term regeneration and functional recovery were evaluated, we found that blockade of CD200R1 had a significant effect impairing the spontaneous functional recovery. Taken together, these results show that CD200R1 has a role in mounting a successful acute inflammatory reaction after injury, and contributes to an effective functional recovery.Universitat Autònoma de Barcelona. Institut de Neurociències 22022-01-0120222022-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/259846https://dx.doi.org/urn:doi:10.3390/cells11111786reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengAgencia Estatal de Investigación https://doi.org/10.13039/501100011033 PID2020-120267RB-I00Instituto de Salud Carlos III https://doi.org/10.13039/501100004587 R21/0017/008European Commission https://doi.org/10.13039/501100000780 101030280open accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:2598462026-06-06T12:50:31Z
dc.title.none.fl_str_mv CD200R1 Contributes to Successful Functional Reinnervation after a Sciatic Nerve Injury
title CD200R1 Contributes to Successful Functional Reinnervation after a Sciatic Nerve Injury
spellingShingle CD200R1 Contributes to Successful Functional Reinnervation after a Sciatic Nerve Injury
Pannunzio, Bruno
CD200R1 blocking antibody
Immune receptor
Macrophages
Regeneration
Functional reinnervation
Wallerian Degeneration
title_short CD200R1 Contributes to Successful Functional Reinnervation after a Sciatic Nerve Injury
title_full CD200R1 Contributes to Successful Functional Reinnervation after a Sciatic Nerve Injury
title_fullStr CD200R1 Contributes to Successful Functional Reinnervation after a Sciatic Nerve Injury
title_full_unstemmed CD200R1 Contributes to Successful Functional Reinnervation after a Sciatic Nerve Injury
title_sort CD200R1 Contributes to Successful Functional Reinnervation after a Sciatic Nerve Injury
dc.creator.none.fl_str_mv Pannunzio, Bruno
Amo Aparicio, Jesús|||0000-0002-1294-6819
Julián, Camila
López Vales, Rubén|||0000-0001-7615-9550
Peluffo, Hugo|||0000-0001-6111-8668
Lago, Natalia|||0000-0002-5631-8558
author Pannunzio, Bruno
author_facet Pannunzio, Bruno
Amo Aparicio, Jesús|||0000-0002-1294-6819
Julián, Camila
López Vales, Rubén|||0000-0001-7615-9550
Peluffo, Hugo|||0000-0001-6111-8668
Lago, Natalia|||0000-0002-5631-8558
author_role author
author2 Amo Aparicio, Jesús|||0000-0002-1294-6819
Julián, Camila
López Vales, Rubén|||0000-0001-7615-9550
Peluffo, Hugo|||0000-0001-6111-8668
Lago, Natalia|||0000-0002-5631-8558
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universitat Autònoma de Barcelona. Institut de Neurociències
dc.subject.none.fl_str_mv CD200R1 blocking antibody
Immune receptor
Macrophages
Regeneration
Functional reinnervation
Wallerian Degeneration
topic CD200R1 blocking antibody
Immune receptor
Macrophages
Regeneration
Functional reinnervation
Wallerian Degeneration
description Activating and inhibitory immune receptors play a critical role in regulating systemic and central nervous system (CNS) immune and inflammatory processes. The CD200R1 immunoreceptor induces a restraining signal modulating inflammation and phagocytosis in the CNS under different inflammatory conditions. However, it remains unknown whether CD200R1 has a role in modulating the inflammatory response after a peripheral nerve injury, an essential component of the successful regeneration. Expression of CD200R1 and its ligand CD200 was analyzed during homeostasis and after a sciatic nerve crush injury in C57Bl/6 mice. The role of CD200R1 in Wallerian Degeneration (WD) and nerve regeneration was studied using a specific antibody against CD200R1 injected into the nerve at the time of injury. We found an upregulation of CD200R1 mRNA after injury whereas CD200 was downregulated acutely after nerve injury. Blockade of CD200R1 significantly reduced the acute entrance of both neutrophils and monocytes from blood after nerve injury. When long term regeneration and functional recovery were evaluated, we found that blockade of CD200R1 had a significant effect impairing the spontaneous functional recovery. Taken together, these results show that CD200R1 has a role in mounting a successful acute inflammatory reaction after injury, and contributes to an effective functional recovery.
publishDate 2022
dc.date.none.fl_str_mv 2
2022-01-01
2022
2022-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/259846
https://dx.doi.org/urn:doi:10.3390/cells11111786
url https://ddd.uab.cat/record/259846
https://dx.doi.org/urn:doi:10.3390/cells11111786
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 PID2020-120267RB-I00
Instituto de Salud Carlos III https://doi.org/10.13039/501100004587 R21/0017/008
European Commission https://doi.org/10.13039/501100000780 101030280
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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