Saliva Sampling Material Matters
There is an increasing interest in measuring biomarkers from saliva, as samples can be obtained noninvasively. This is particularly true in pigs where blood sampling is technically complicated and stressful. In pigs, studies regarding how the absorbent material used for collection affects analytical...
| Autores: | , , , , , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Fecha de publicación: | 2023 |
| País: | España |
| Institución: | Universitat Autònoma de Barcelona |
| Repositorio: | Dipòsit Digital de Documents de la UAB |
| Idioma: | inglés |
| OAI Identifier: | oai:ddd.uab.cat:286989 |
| Acceso en línea: | https://ddd.uab.cat/record/286989 https://dx.doi.org/urn:doi:10.3390/ani13243757 |
| Access Level: | acceso abierto |
| Palabra clave: | Analytes Collection material Pig Stimulated saliva |
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Saliva Sampling Material MattersEffects on the Results of Saliva Analysis in PigsOrtín-Bustillo, AlbaBotía, María|||0000-0002-0390-2953Lopez Arjona, Marina|||0000-0003-3025-440XPardo-Marín, Luis|||0000-0001-5398-1162Martínez-Subiela, Silvia|||0000-0002-1524-1558López-Martínez, María José|||0000-0003-4159-037XTvarijonaviciute, Asta|||0000-0002-5323-5001Muñoz Prieto, Alberto|||0000-0001-6865-8712Rubio, Camila Peres|||0000-0002-9293-4147Martínez-Miró, Silvia|||0000-0002-1350-0886Escribano Tortosa, Damián|||0000-0001-9439-6779Tecles, Fernando|||0000-0002-7635-533XAnalytesCollection materialPigStimulated salivaThere is an increasing interest in measuring biomarkers from saliva, as samples can be obtained noninvasively. This is particularly true in pigs where blood sampling is technically complicated and stressful. In pigs, studies regarding how the absorbent material used for collection affects analytical results are scarce. The aim of this study was to evaluate how the material used for saliva collection can affect the results of analyses for the different types of biomarkers that can be measured in the saliva of pigs. In this report, polypropylene sponges gave higher sample saliva volumes and, in some salivary analytes, provided different values compared with cotton rolls. Therefore, it can be concluded that the type of material used can influence the results of saliva analysis. The use of saliva as a biological sample from pigs is of high practical interest because blood collection from pigs is difficult and stressful. In this study, the influence of two different materials, a cotton roll and a polypropylene sponge, in porcine saliva collection was evaluated. For this purpose, the effect of the material used for sampling was evaluated in a panel of 13 analytes, including those related to stress (cortisol and oxytocin), inflammation and immunity (adenosine deaminase, haptoglobin and myeloperoxidase), redox homeostasis (the cupric reducing ability of saliva, the ferric reducing activity of saliva, and the Trolox equivalent antioxidant capacity), and sepsis (procalcitonin), as well as other routine analytes related to metabolism and different tissues and organs, such as lactate dehydrogenase, creatine kinase, urea, and total protein concentration. The polypropylene sponge provided a higher sample volume than the cotton roll. Although the results of some salivary analytes were equivalent for both materials, other analytes, such as creatine kinase, haptoglobin and total proteins, showed significant differences depending on the material used for saliva collection. Therefore, the type of material used for salivary collection in pigs should be considered when interpreting the results of analyses of the salivary analytes. 22023-01-0120232023-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/286989https://dx.doi.org/urn:doi:10.3390/ani13243757reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengMinisterio de Ciencia e Innovación https://doi.org/10.13039/501100004837 TED2021-130685B-I00Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 FJC2021-047105-IMinisterio de Economía y Competitividad https://doi.org/10.13039/501100003329 FJC2019-042475-Iopen accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:2869892026-06-06T12:50:31Z |
| dc.title.none.fl_str_mv |
Saliva Sampling Material Matters Effects on the Results of Saliva Analysis in Pigs |
| title |
Saliva Sampling Material Matters |
| spellingShingle |
Saliva Sampling Material Matters Ortín-Bustillo, Alba Analytes Collection material Pig Stimulated saliva |
| title_short |
Saliva Sampling Material Matters |
| title_full |
Saliva Sampling Material Matters |
| title_fullStr |
Saliva Sampling Material Matters |
| title_full_unstemmed |
Saliva Sampling Material Matters |
| title_sort |
Saliva Sampling Material Matters |
| dc.creator.none.fl_str_mv |
Ortín-Bustillo, Alba Botía, María|||0000-0002-0390-2953 Lopez Arjona, Marina|||0000-0003-3025-440X Pardo-Marín, Luis|||0000-0001-5398-1162 Martínez-Subiela, Silvia|||0000-0002-1524-1558 López-Martínez, María José|||0000-0003-4159-037X Tvarijonaviciute, Asta|||0000-0002-5323-5001 Muñoz Prieto, Alberto|||0000-0001-6865-8712 Rubio, Camila Peres|||0000-0002-9293-4147 Martínez-Miró, Silvia|||0000-0002-1350-0886 Escribano Tortosa, Damián|||0000-0001-9439-6779 Tecles, Fernando|||0000-0002-7635-533X |
| author |
Ortín-Bustillo, Alba |
| author_facet |
Ortín-Bustillo, Alba Botía, María|||0000-0002-0390-2953 Lopez Arjona, Marina|||0000-0003-3025-440X Pardo-Marín, Luis|||0000-0001-5398-1162 Martínez-Subiela, Silvia|||0000-0002-1524-1558 López-Martínez, María José|||0000-0003-4159-037X Tvarijonaviciute, Asta|||0000-0002-5323-5001 Muñoz Prieto, Alberto|||0000-0001-6865-8712 Rubio, Camila Peres|||0000-0002-9293-4147 Martínez-Miró, Silvia|||0000-0002-1350-0886 Escribano Tortosa, Damián|||0000-0001-9439-6779 Tecles, Fernando|||0000-0002-7635-533X |
| author_role |
author |
| author2 |
Botía, María|||0000-0002-0390-2953 Lopez Arjona, Marina|||0000-0003-3025-440X Pardo-Marín, Luis|||0000-0001-5398-1162 Martínez-Subiela, Silvia|||0000-0002-1524-1558 López-Martínez, María José|||0000-0003-4159-037X Tvarijonaviciute, Asta|||0000-0002-5323-5001 Muñoz Prieto, Alberto|||0000-0001-6865-8712 Rubio, Camila Peres|||0000-0002-9293-4147 Martínez-Miró, Silvia|||0000-0002-1350-0886 Escribano Tortosa, Damián|||0000-0001-9439-6779 Tecles, Fernando|||0000-0002-7635-533X |
| author2_role |
author author author author author author author author author author author |
| dc.subject.none.fl_str_mv |
Analytes Collection material Pig Stimulated saliva |
| topic |
Analytes Collection material Pig Stimulated saliva |
| description |
There is an increasing interest in measuring biomarkers from saliva, as samples can be obtained noninvasively. This is particularly true in pigs where blood sampling is technically complicated and stressful. In pigs, studies regarding how the absorbent material used for collection affects analytical results are scarce. The aim of this study was to evaluate how the material used for saliva collection can affect the results of analyses for the different types of biomarkers that can be measured in the saliva of pigs. In this report, polypropylene sponges gave higher sample saliva volumes and, in some salivary analytes, provided different values compared with cotton rolls. Therefore, it can be concluded that the type of material used can influence the results of saliva analysis. The use of saliva as a biological sample from pigs is of high practical interest because blood collection from pigs is difficult and stressful. In this study, the influence of two different materials, a cotton roll and a polypropylene sponge, in porcine saliva collection was evaluated. For this purpose, the effect of the material used for sampling was evaluated in a panel of 13 analytes, including those related to stress (cortisol and oxytocin), inflammation and immunity (adenosine deaminase, haptoglobin and myeloperoxidase), redox homeostasis (the cupric reducing ability of saliva, the ferric reducing activity of saliva, and the Trolox equivalent antioxidant capacity), and sepsis (procalcitonin), as well as other routine analytes related to metabolism and different tissues and organs, such as lactate dehydrogenase, creatine kinase, urea, and total protein concentration. The polypropylene sponge provided a higher sample volume than the cotton roll. Although the results of some salivary analytes were equivalent for both materials, other analytes, such as creatine kinase, haptoglobin and total proteins, showed significant differences depending on the material used for saliva collection. Therefore, the type of material used for salivary collection in pigs should be considered when interpreting the results of analyses of the salivary analytes. |
| publishDate |
2023 |
| dc.date.none.fl_str_mv |
2 2023-01-01 2023 2023-01-01 |
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Article http://purl.org/coar/resource_type/c_6501 VoR http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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info:eu-repo/semantics/article |
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article |
| dc.identifier.none.fl_str_mv |
https://ddd.uab.cat/record/286989 https://dx.doi.org/urn:doi:10.3390/ani13243757 |
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https://ddd.uab.cat/record/286989 https://dx.doi.org/urn:doi:10.3390/ani13243757 |
| dc.language.none.fl_str_mv |
Inglés eng |
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Inglés |
| language |
eng |
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Ministerio de Ciencia e Innovación https://doi.org/10.13039/501100004837 TED2021-130685B-I00 Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 FJC2021-047105-I Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 FJC2019-042475-I |
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open access http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by/4.0/ |
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info:eu-repo/semantics/openAccess |
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open access http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by/4.0/ |
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openAccess |
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