3D printing of hierarchical porous biomimetic hydroxyapatite scaffolds: Adding concavities to the convex filaments
Porosity plays a key role on the osteogenic performance of bone scaffolds. Direct Ink Writing (DIW) allows the design of customized synthetic bone grafts with patient-specific architecture and controlled macroporosity. Being an extrusion-based technique, the scaffolds obtained are formed by arrays o...
| Autores: | , , , |
|---|---|
| Tipo de documento: | artigo |
| Data de publicação: | 2021 |
| País: | España |
| Recursos: | Universitat Politècnica de Catalunya (UPC) |
| Repositório: | UPCommons. Portal del coneixement obert de la UPC |
| Idioma: | inglês |
| OAI Identifier: | oai:upcommons.upc.edu:2117/355149 |
| Acesso em linha: | https://hdl.handle.net/2117/355149 https://dx.doi.org/10.1016/j.actbio.2021.07.071 |
| Access Level: | Acceso aberto |
| Palavra-chave: | Hydroxyapatite Bone regeneration Three-dimensional printing 3D printing Gelatin Biomimetic Concavity Porous filament Hidroxiapatita Ossos--Regeneració Impressió 3D Àrees temàtiques de la UPC::Enginyeria dels materials |
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3D printing of hierarchical porous biomimetic hydroxyapatite scaffolds: Adding concavities to the convex filamentsKonka, Joanna Magdalena|||0000-0001-6593-8532Buxadera Palomero, Judit|||0000-0003-0897-2093Español Pons, Montserrat|||0000-0001-7510-0602Ginebra Molins, Maria Pau|||0000-0002-4700-5621HydroxyapatiteBone regenerationThree-dimensional printing3D printingHydroxyapatiteGelatinBiomimeticConcavityBone regenerationPorous filamentHidroxiapatitaOssos--RegeneracióImpressió 3DÀrees temàtiques de la UPC::Enginyeria dels materialsPorosity plays a key role on the osteogenic performance of bone scaffolds. Direct Ink Writing (DIW) allows the design of customized synthetic bone grafts with patient-specific architecture and controlled macroporosity. Being an extrusion-based technique, the scaffolds obtained are formed by arrays of cylindrical filaments, and therefore have convex surfaces. This may represent a serious limitation, as the role of surface curvature and more specifically the stimulating role of concave surfaces in osteoinduction and bone growth has been recently highlighted. Hence the need to design strategies that allow the introduction of concave pores in DIW scaffolds. In the current study, we propose to add gelatin microspheres as a sacrificial material in a self-setting calcium phosphate ink. Neither the phase transformation responsible for the hardening of the scaffold nor the formation of characteristic network of needle-like hydroxyapatite crystals was affected by the addition of gelatin microspheres. The partial dissolution of the gelatin resulted in the creation of spherical pores throughout the filaments and exposed on the surface, increasing filament porosity from 0.2 % to 67.9 %. Moreover, the presence of retained gelatin proved to have a significant effect on the mechanical properties, reducing the strength but simultaneously giving the scaffolds an elastic behavior, despite the high content of ceramic as a continuous phase. Notwithstanding the inherent difficulty of in vitro cultures with this highly reactive material an enhancement of MG-63 cell proliferation, as well as better spreading of hMSCs was recorded on the developed scaffolds.Peer ReviewedElsevier20212021-01-0120212021-11-02journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/2117/355149https://dx.doi.org/10.1016/j.actbio.2021.07.071reponame:UPCommons. Portal del coneixement obert de la UPCinstname:Universitat Politècnica de Catalunya (UPC)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:upcommons.upc.edu:2117/3551492026-05-27T15:37:01Z |
| dc.title.none.fl_str_mv |
3D printing of hierarchical porous biomimetic hydroxyapatite scaffolds: Adding concavities to the convex filaments |
| title |
3D printing of hierarchical porous biomimetic hydroxyapatite scaffolds: Adding concavities to the convex filaments |
| spellingShingle |
3D printing of hierarchical porous biomimetic hydroxyapatite scaffolds: Adding concavities to the convex filaments Konka, Joanna Magdalena|||0000-0001-6593-8532 Hydroxyapatite Bone regeneration Three-dimensional printing 3D printing Hydroxyapatite Gelatin Biomimetic Concavity Bone regeneration Porous filament Hidroxiapatita Ossos--Regeneració Impressió 3D Àrees temàtiques de la UPC::Enginyeria dels materials |
| title_short |
3D printing of hierarchical porous biomimetic hydroxyapatite scaffolds: Adding concavities to the convex filaments |
| title_full |
3D printing of hierarchical porous biomimetic hydroxyapatite scaffolds: Adding concavities to the convex filaments |
| title_fullStr |
3D printing of hierarchical porous biomimetic hydroxyapatite scaffolds: Adding concavities to the convex filaments |
| title_full_unstemmed |
3D printing of hierarchical porous biomimetic hydroxyapatite scaffolds: Adding concavities to the convex filaments |
| title_sort |
3D printing of hierarchical porous biomimetic hydroxyapatite scaffolds: Adding concavities to the convex filaments |
| dc.creator.none.fl_str_mv |
Konka, Joanna Magdalena|||0000-0001-6593-8532 Buxadera Palomero, Judit|||0000-0003-0897-2093 Español Pons, Montserrat|||0000-0001-7510-0602 Ginebra Molins, Maria Pau|||0000-0002-4700-5621 |
| author |
Konka, Joanna Magdalena|||0000-0001-6593-8532 |
| author_facet |
Konka, Joanna Magdalena|||0000-0001-6593-8532 Buxadera Palomero, Judit|||0000-0003-0897-2093 Español Pons, Montserrat|||0000-0001-7510-0602 Ginebra Molins, Maria Pau|||0000-0002-4700-5621 |
| author_role |
author |
| author2 |
Buxadera Palomero, Judit|||0000-0003-0897-2093 Español Pons, Montserrat|||0000-0001-7510-0602 Ginebra Molins, Maria Pau|||0000-0002-4700-5621 |
| author2_role |
author author author |
| dc.subject.none.fl_str_mv |
Hydroxyapatite Bone regeneration Three-dimensional printing 3D printing Hydroxyapatite Gelatin Biomimetic Concavity Bone regeneration Porous filament Hidroxiapatita Ossos--Regeneració Impressió 3D Àrees temàtiques de la UPC::Enginyeria dels materials |
| topic |
Hydroxyapatite Bone regeneration Three-dimensional printing 3D printing Hydroxyapatite Gelatin Biomimetic Concavity Bone regeneration Porous filament Hidroxiapatita Ossos--Regeneració Impressió 3D Àrees temàtiques de la UPC::Enginyeria dels materials |
| description |
Porosity plays a key role on the osteogenic performance of bone scaffolds. Direct Ink Writing (DIW) allows the design of customized synthetic bone grafts with patient-specific architecture and controlled macroporosity. Being an extrusion-based technique, the scaffolds obtained are formed by arrays of cylindrical filaments, and therefore have convex surfaces. This may represent a serious limitation, as the role of surface curvature and more specifically the stimulating role of concave surfaces in osteoinduction and bone growth has been recently highlighted. Hence the need to design strategies that allow the introduction of concave pores in DIW scaffolds. In the current study, we propose to add gelatin microspheres as a sacrificial material in a self-setting calcium phosphate ink. Neither the phase transformation responsible for the hardening of the scaffold nor the formation of characteristic network of needle-like hydroxyapatite crystals was affected by the addition of gelatin microspheres. The partial dissolution of the gelatin resulted in the creation of spherical pores throughout the filaments and exposed on the surface, increasing filament porosity from 0.2 % to 67.9 %. Moreover, the presence of retained gelatin proved to have a significant effect on the mechanical properties, reducing the strength but simultaneously giving the scaffolds an elastic behavior, despite the high content of ceramic as a continuous phase. Notwithstanding the inherent difficulty of in vitro cultures with this highly reactive material an enhancement of MG-63 cell proliferation, as well as better spreading of hMSCs was recorded on the developed scaffolds. |
| publishDate |
2021 |
| dc.date.none.fl_str_mv |
2021 2021-01-01 2021 2021-11-02 |
| dc.type.none.fl_str_mv |
journal article http://purl.org/coar/resource_type/c_6501 VoR http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| dc.type.openaire.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| dc.identifier.none.fl_str_mv |
https://hdl.handle.net/2117/355149 https://dx.doi.org/10.1016/j.actbio.2021.07.071 |
| url |
https://hdl.handle.net/2117/355149 https://dx.doi.org/10.1016/j.actbio.2021.07.071 |
| dc.language.none.fl_str_mv |
Inglés eng |
| language_invalid_str_mv |
Inglés |
| language |
eng |
| dc.rights.none.fl_str_mv |
open access http://purl.org/coar/access_right/c_abf2 Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| dc.rights.openaire.fl_str_mv |
info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
open access http://purl.org/coar/access_right/c_abf2 Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
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openAccess |
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application/pdf |
| dc.publisher.none.fl_str_mv |
Elsevier |
| publisher.none.fl_str_mv |
Elsevier |
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reponame:UPCommons. Portal del coneixement obert de la UPC instname:Universitat Politècnica de Catalunya (UPC) |
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Universitat Politècnica de Catalunya (UPC) |
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UPCommons. Portal del coneixement obert de la UPC |
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UPCommons. Portal del coneixement obert de la UPC |
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15,301603 |