Transient expression systems in plants: Potentialities and constraints

18 p.

Detalles Bibliográficos
Autor: Canto, Tomás
Tipo de recurso: artículo
Estado:Versión enviada para evaluación y publicación
Fecha de publicación:2016
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/135535
Acceso en línea:http://hdl.handle.net/10261/135535
Access Level:acceso abierto
Palabra clave:Transient expression by agroinfiltration
Transient expression from plant virus vectors
Transient expression in plants by biolistic bombardment
Transient expression systems in plants
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spelling Transient expression systems in plants: Potentialities and constraintsCanto, TomásTransient expression by agroinfiltrationTransient expression from plant virus vectorsTransient expression in plants by biolistic bombardmentTransient expression systems in plants18 p.Plants have been used from old to extract and isolate by different means the products of interest that they store. In recent years new techniques have emerged that allow the use of plants as factories to overexpress transiently and often efficiently, specific genes of interest, either endogenous or foreign, in their native form or modified. These techniques allow and facilitate the targeted purification of gene products for research and commercial purposes without resorting to lengthy, time-consuming and sometimes challenging plant stable transformations, while avoiding some of their associated regulatory constraints. In this chapter we describe the main strategies available for the transient expression of gene sequences and their encoded products in plants. We discuss biological issues affecting transient expression, including resistance responses elicited by the plant against sequences that it recognizes naturally as foreign, and ways to neutralize them. We also discuss the relative advantages of each expression strategy as well as their inherent drawbacks and technical limitations, and how to partially prevent or overcome them, whenever possibleTC’s research is currently supported by grant PJ009461 from the Rural Development Administration (RDA) of the Republic of Korea, in cooperation with the Spanish Council for Scientific Research (CSIC), and also by grant BIO2013-47940-R from the Spanish Ministry of Economy and Competitiveness (MEC).NoKluwer Academic PublishersRural Development Administration (South Korea)Consejo Superior de Investigaciones Científicas (España)Ministerio de Economía y Competitividad (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201620162016info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Preprintinfo:eu-repo/semantics/submittedVersionhttp://hdl.handle.net/10261/135535reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/ 10.1007/978-3-319-27216-0_18Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1355352026-05-22T06:33:51Z
dc.title.none.fl_str_mv Transient expression systems in plants: Potentialities and constraints
title Transient expression systems in plants: Potentialities and constraints
spellingShingle Transient expression systems in plants: Potentialities and constraints
Canto, Tomás
Transient expression by agroinfiltration
Transient expression from plant virus vectors
Transient expression in plants by biolistic bombardment
Transient expression systems in plants
title_short Transient expression systems in plants: Potentialities and constraints
title_full Transient expression systems in plants: Potentialities and constraints
title_fullStr Transient expression systems in plants: Potentialities and constraints
title_full_unstemmed Transient expression systems in plants: Potentialities and constraints
title_sort Transient expression systems in plants: Potentialities and constraints
dc.creator.none.fl_str_mv Canto, Tomás
author Canto, Tomás
author_facet Canto, Tomás
author_role author
dc.contributor.none.fl_str_mv Rural Development Administration (South Korea)
Consejo Superior de Investigaciones Científicas (España)
Ministerio de Economía y Competitividad (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Transient expression by agroinfiltration
Transient expression from plant virus vectors
Transient expression in plants by biolistic bombardment
Transient expression systems in plants
topic Transient expression by agroinfiltration
Transient expression from plant virus vectors
Transient expression in plants by biolistic bombardment
Transient expression systems in plants
description 18 p.
publishDate 2016
dc.date.none.fl_str_mv 2016
2016
2016
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Preprint
info:eu-repo/semantics/submittedVersion
format article
status_str submittedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/135535
url http://hdl.handle.net/10261/135535
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/ 10.1007/978-3-319-27216-0_18
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Kluwer Academic Publishers
publisher.none.fl_str_mv Kluwer Academic Publishers
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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