RNA-Seq transcriptomic profiling of primary murine microglia treated with LPS or LPS+IFNγ

Microglia, the main resident immune cells in the CNS, are thought to participate in the pathogenesis of various neurological disorders. LPS and LPS + IFNγ are stimuli that are widely used to activate microglia. However, the transcriptomic profiles of microglia treated with LPS and LPS + IFNγ have no...

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Autores: Pulido Salgado, Marta, Vidal Taboada, José Manuel, García-Díaz Barriga, Gerardo, Solà i Subirana, Carme, Saura Martí, Josep
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2018
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/156637
Acceso en línea:https://hdl.handle.net/2445/156637
Access Level:acceso abierto
Palabra clave:Micròglia
Malalties del sistema nerviós central
RNA
Microglia
Central nervous system diseases
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spelling RNA-Seq transcriptomic profiling of primary murine microglia treated with LPS or LPS+IFNγPulido Salgado, MartaVidal Taboada, José ManuelGarcía-Díaz Barriga, GerardoSolà i Subirana, CarmeSaura Martí, JosepMicrògliaMalalties del sistema nerviós centralRNAMicrogliaCentral nervous system diseasesRNAMicroglia, the main resident immune cells in the CNS, are thought to participate in the pathogenesis of various neurological disorders. LPS and LPS + IFNγ are stimuli that are widely used to activate microglia. However, the transcriptomic profiles of microglia treated with LPS and LPS + IFNγ have not been properly compared. Here, we treated murine primary microglial cultures with LPS or LPS + IFNγ for 6 hours and then performed RNA-Sequencing. Gene expression patterns induced by the treatments were obtained by WGCNA and 11 different expression profiles were found, showing differential responses to LPS and LPS + IFNγ in many genes. Interestingly, a subset of genes involved in Parkinson's, Alzheimer's and Huntington's disease were downregulated by both treatments. By DESeq analysis we found differentially upregulated and downregulated genes that confirmed LPS and LPS + IFNγ as inducers of microglial pro-inflammatory responses, but also highlighted their involvement in specific cell functions. In response to LPS, microglia tended to be more proliferative, pro-inflammatory and phagocytic; whereas LPS + IFNγ inhibited genes were involved in pain, cell division and, unexpectedly, production of some inflammatory mediators. In summary, this study provides a detailed description of the transcriptome of LPS- and LPS + IFNγ treated primary microglial cultures. It may be useful to determine whether these in vitro phenotypes resemble microglia in in vivo pathological conditions.Nature Publishing Group2020202020182020info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion21 p.application/pdfhttps://hdl.handle.net/2445/156637Articles publicats en revistes (Biomedicina)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: https://doi.org/10.1038/s41598-018-34412-9Scientific Reports, 2018, vol. 8, p. 16906https://doi.org/10.1038/s41598-018-34412-9cc-by (c) Pulido Salgado, Marta et al., 2018http://creativecommons.org/licenses/by/3.0/esinfo:eu-repo/semantics/openAccessoai:recercat.cat:2445/1566372026-05-29T05:05:01Z
dc.title.none.fl_str_mv RNA-Seq transcriptomic profiling of primary murine microglia treated with LPS or LPS+IFNγ
title RNA-Seq transcriptomic profiling of primary murine microglia treated with LPS or LPS+IFNγ
spellingShingle RNA-Seq transcriptomic profiling of primary murine microglia treated with LPS or LPS+IFNγ
Pulido Salgado, Marta
Micròglia
Malalties del sistema nerviós central
RNA
Microglia
Central nervous system diseases
RNA
title_short RNA-Seq transcriptomic profiling of primary murine microglia treated with LPS or LPS+IFNγ
title_full RNA-Seq transcriptomic profiling of primary murine microglia treated with LPS or LPS+IFNγ
title_fullStr RNA-Seq transcriptomic profiling of primary murine microglia treated with LPS or LPS+IFNγ
title_full_unstemmed RNA-Seq transcriptomic profiling of primary murine microglia treated with LPS or LPS+IFNγ
title_sort RNA-Seq transcriptomic profiling of primary murine microglia treated with LPS or LPS+IFNγ
dc.creator.none.fl_str_mv Pulido Salgado, Marta
Vidal Taboada, José Manuel
García-Díaz Barriga, Gerardo
Solà i Subirana, Carme
Saura Martí, Josep
author Pulido Salgado, Marta
author_facet Pulido Salgado, Marta
Vidal Taboada, José Manuel
García-Díaz Barriga, Gerardo
Solà i Subirana, Carme
Saura Martí, Josep
author_role author
author2 Vidal Taboada, José Manuel
García-Díaz Barriga, Gerardo
Solà i Subirana, Carme
Saura Martí, Josep
author2_role author
author
author
author
dc.subject.none.fl_str_mv Micròglia
Malalties del sistema nerviós central
RNA
Microglia
Central nervous system diseases
RNA
topic Micròglia
Malalties del sistema nerviós central
RNA
Microglia
Central nervous system diseases
RNA
description Microglia, the main resident immune cells in the CNS, are thought to participate in the pathogenesis of various neurological disorders. LPS and LPS + IFNγ are stimuli that are widely used to activate microglia. However, the transcriptomic profiles of microglia treated with LPS and LPS + IFNγ have not been properly compared. Here, we treated murine primary microglial cultures with LPS or LPS + IFNγ for 6 hours and then performed RNA-Sequencing. Gene expression patterns induced by the treatments were obtained by WGCNA and 11 different expression profiles were found, showing differential responses to LPS and LPS + IFNγ in many genes. Interestingly, a subset of genes involved in Parkinson's, Alzheimer's and Huntington's disease were downregulated by both treatments. By DESeq analysis we found differentially upregulated and downregulated genes that confirmed LPS and LPS + IFNγ as inducers of microglial pro-inflammatory responses, but also highlighted their involvement in specific cell functions. In response to LPS, microglia tended to be more proliferative, pro-inflammatory and phagocytic; whereas LPS + IFNγ inhibited genes were involved in pain, cell division and, unexpectedly, production of some inflammatory mediators. In summary, this study provides a detailed description of the transcriptome of LPS- and LPS + IFNγ treated primary microglial cultures. It may be useful to determine whether these in vitro phenotypes resemble microglia in in vivo pathological conditions.
publishDate 2018
dc.date.none.fl_str_mv 2018
2020
2020
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/156637
url https://hdl.handle.net/2445/156637
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1038/s41598-018-34412-9
Scientific Reports, 2018, vol. 8, p. 16906
https://doi.org/10.1038/s41598-018-34412-9
dc.rights.none.fl_str_mv cc-by (c) Pulido Salgado, Marta et al., 2018
http://creativecommons.org/licenses/by/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Pulido Salgado, Marta et al., 2018
http://creativecommons.org/licenses/by/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 21 p.
application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv Articles publicats en revistes (Biomedicina)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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