Role of Extracellular Vesicles in Chronic Post-Embolic Pulmonary Hypertension: Data from an Experimental Animal Model and Patients

This article belongs to the Special Issue Molecular and Translational Research in Cardiovascular Disease.

Detalles Bibliográficos
Autores: Mendoza-Zambrano, Elva María, Sánchez-López, Verónica, Gómez-Rodríguez, Belén, García-Lunar, Inés, Pereda-Arnau, Daniel, Jara Palomares, Luis, Elías-Hernández, Teresa, García-Álvarez, Ana, Otero-Candelera, Remedios
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2025
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/400135
Acceso en línea:http://hdl.handle.net/10261/400135
https://api.elsevier.com/content/abstract/scopus_id/105009160673
Access Level:acceso abierto
Palabra clave:Chronic thromboembolic pulmonary hypertension
Endothelial dysfunction
Extracellular vesicles
Pulmonary embolism
Vascular remodeling
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network_name_str España
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dc.title.none.fl_str_mv Role of Extracellular Vesicles in Chronic Post-Embolic Pulmonary Hypertension: Data from an Experimental Animal Model and Patients
title Role of Extracellular Vesicles in Chronic Post-Embolic Pulmonary Hypertension: Data from an Experimental Animal Model and Patients
spellingShingle Role of Extracellular Vesicles in Chronic Post-Embolic Pulmonary Hypertension: Data from an Experimental Animal Model and Patients
Mendoza-Zambrano, Elva María
Chronic thromboembolic pulmonary hypertension
Endothelial dysfunction
Extracellular vesicles
Pulmonary embolism
Vascular remodeling
title_short Role of Extracellular Vesicles in Chronic Post-Embolic Pulmonary Hypertension: Data from an Experimental Animal Model and Patients
title_full Role of Extracellular Vesicles in Chronic Post-Embolic Pulmonary Hypertension: Data from an Experimental Animal Model and Patients
title_fullStr Role of Extracellular Vesicles in Chronic Post-Embolic Pulmonary Hypertension: Data from an Experimental Animal Model and Patients
title_full_unstemmed Role of Extracellular Vesicles in Chronic Post-Embolic Pulmonary Hypertension: Data from an Experimental Animal Model and Patients
title_sort Role of Extracellular Vesicles in Chronic Post-Embolic Pulmonary Hypertension: Data from an Experimental Animal Model and Patients
dc.creator.none.fl_str_mv Mendoza-Zambrano, Elva María
Sánchez-López, Verónica
Gómez-Rodríguez, Belén
García-Lunar, Inés
Pereda-Arnau, Daniel
Jara Palomares, Luis
Elías-Hernández, Teresa
García-Álvarez, Ana
Otero-Candelera, Remedios
author Mendoza-Zambrano, Elva María
author_facet Mendoza-Zambrano, Elva María
Sánchez-López, Verónica
Gómez-Rodríguez, Belén
García-Lunar, Inés
Pereda-Arnau, Daniel
Jara Palomares, Luis
Elías-Hernández, Teresa
García-Álvarez, Ana
Otero-Candelera, Remedios
author_role author
author2 Sánchez-López, Verónica
Gómez-Rodríguez, Belén
García-Lunar, Inés
Pereda-Arnau, Daniel
Jara Palomares, Luis
Elías-Hernández, Teresa
García-Álvarez, Ana
Otero-Candelera, Remedios
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Instituto de Salud Carlos III
Sociedad Española de Neumología y Cirugía Torácica
Junta de Andalucía
Ministerio de Ciencia e Innovación (España)
Agencia Estatal de Investigación (España)
European Commission
Mendoza-Zambrano, Elva María [0000-0002-4516-9059]
Pereda-Arnau, Daniel [0000-0002-0526-8722]
Jara Palomares, Luis [0000-0002-4125-3376]
Elías-Hernández, Teresa [0000-0002-6181-3617]
Otero-Candelera, Remedios [0000-0002-2265-1221]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Chronic thromboembolic pulmonary hypertension
Endothelial dysfunction
Extracellular vesicles
Pulmonary embolism
Vascular remodeling
topic Chronic thromboembolic pulmonary hypertension
Endothelial dysfunction
Extracellular vesicles
Pulmonary embolism
Vascular remodeling
description This article belongs to the Special Issue Molecular and Translational Research in Cardiovascular Disease.
publishDate 2025
dc.date.none.fl_str_mv 2025
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
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status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/400135
https://api.elsevier.com/content/abstract/scopus_id/105009160673
url http://hdl.handle.net/10261/400135
https://api.elsevier.com/content/abstract/scopus_id/105009160673
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI//CEX2020-001041-S
The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI https://doi.org/10.3390/biomedicines13061499
https://doi.org/10.3390/biomedicines13061499

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eu_rights_str_mv openAccess
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dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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spelling Role of Extracellular Vesicles in Chronic Post-Embolic Pulmonary Hypertension: Data from an Experimental Animal Model and PatientsMendoza-Zambrano, Elva MaríaSánchez-López, VerónicaGómez-Rodríguez, BelénGarcía-Lunar, InésPereda-Arnau, DanielJara Palomares, LuisElías-Hernández, TeresaGarcía-Álvarez, AnaOtero-Candelera, RemediosChronic thromboembolic pulmonary hypertensionEndothelial dysfunctionExtracellular vesiclesPulmonary embolismVascular remodelingThis article belongs to the Special Issue Molecular and Translational Research in Cardiovascular Disease.[Background:] The pathogenesis of chronic thromboembolic pulmonary hypertension (CTEPH) involves a multifaceted interplay of factors, including incomplete thrombus resolution, endothelial dysfunction, and vascular remodeling. Recent studies have highlighted the role of extracellular vesicles (EVs) in vascular diseases, suggesting their potential involvement in CTEPH progression. This study aims to investigate the role of EVs from various cellular sources in the development of CTEPH. Methods[Methods:] An experimental study was conducted using 11 male three-month-old Large-White pigs. The EVs of endothelial origin (EEVs; CD146+), leukocyte-derived EVs (LEVs; CD45+, CD44+), and consistent with mesenchymal-origin EVs (CD90+, CD105+) were quantified. Measurements were taken at baseline, after the first embolization, and prior to each subsequent weekly embolization. Embolizations were repeated until chronic pulmonary hypertension (PH) was generated. Based on these findings, a clinical case-control study was performed involving nine patients previously diagnosed with CTEPH and 18 patients with pulmonary embolism who did not develop CTEPH after two years of follow-up.[Results:] The experimental study, consistent with the mesenchymal-origin EVs, exhibited a progressive decrease below baseline levels; LEVs decreased after PH was established, while EEVs remained elevated throughout the study. Subsequently, in the clinical case-control study, CD45+ LEVs emerged as a significant association of CTEPH, with an odds ratio (OR) of 21.25 (95% CI: 1.91–236.00; p = 0.013).[Conclusions:] Inflammation involving LEVs and EEVs plays a crucial role in sustaining the vascular alterations leading to pulmonary vasculature remodeling in CTEPH.This work was supported by the Carlos III Health Institute [PI11/02308]; Spanish Society of Pulmonology and Thoracic Surgery, SEPAR [124/2011]; Ministry of Economy, Innovation, Science and Employment of the Junta de Andalucía [CVI-6654/2010]; CNIC is supported by the Carlos III Health Institute (ISCIII), the Ministerio de Ciencia e Innovación (MICIU) and the Pro CNIC Foundation, and is a Severo Ochoa Center of Excellence (grant CEX2020-001041-S funded by MICIN/AEI/10.13039/501100011033). I.G.-L. is supported by project "PI24/00260", funded by Carlos III Health Institute (ISCIII) and co-funded by the European Union.With funding form the Spanish government through the "Severa Ochoa Centre of Excellence" accreditation (CEX2020-001041-S).Peer reviewedMultidisciplinary Digital Publishing InstituteInstituto de Salud Carlos IIISociedad Española de Neumología y Cirugía TorácicaJunta de AndalucíaMinisterio de Ciencia e Innovación (España)Agencia Estatal de Investigación (España)European CommissionMendoza-Zambrano, Elva María [0000-0002-4516-9059]Pereda-Arnau, Daniel [0000-0002-0526-8722]Jara Palomares, Luis [0000-0002-4125-3376]Elías-Hernández, Teresa [0000-0002-6181-3617]Otero-Candelera, Remedios [0000-0002-2265-1221]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202520252025info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/400135https://api.elsevier.com/content/abstract/scopus_id/105009160673reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI//CEX2020-001041-SThe underlying dataset has been published as supplementary material of the article in the publisher platform at DOI https://doi.org/10.3390/biomedicines13061499https://doi.org/10.3390/biomedicines13061499Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/4001352026-05-22T06:33:51Z
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