The fission yeast cell wall stress sensor-like proteins Mtl2 and Wsc1 act by turning on the GTPase Rho1p but act independently of the cell wall integrity pathway

This is an open access article under the terms of the Creative Commons Attribution License.

Detalles Bibliográficos
Autores: Cruz, Sandra, Muñoz Félix, Sofía, Manjón, Elvira, García, Patricia, Sánchez, Yolanda
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2013
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/116085
Acceso en línea:http://hdl.handle.net/10261/116085
Access Level:acceso abierto
Palabra clave:Fission yeast
MAPK pathway
Cell wall sensor
Cell walls
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spelling The fission yeast cell wall stress sensor-like proteins Mtl2 and Wsc1 act by turning on the GTPase Rho1p but act independently of the cell wall integrity pathwayCruz, SandraMuñoz Félix, SofíaManjón, ElviraGarcía, PatriciaSánchez, YolandaFission yeastMAPK pathwayCell wall sensorCell wallsThis is an open access article under the terms of the Creative Commons Attribution License.Sensing stressful conditions that affect the cell wall reorganization is important for yeast survival. Here, we studied two proteins SpWsc1p and SpMtl2p with structural features indicative of plasma membrane-associated cell wall sensors. We found that Mtl2p and Wsc1p act by turning on the Rho1p GTPase. Each gene could be deleted individually without affecting viability, but the deletion of both was lethal and this phenotype was rescued by overexpression of the genes encoding either Rho1p or its GDP/GTP exchange factors (GEFs). In addition, wsc1Δ and mtl2Δ cells showed a low level of Rho1p-GTP under cell wall stress. Mtl2p-GFP (green fluorescent protein) localized to the cell periphery and was necessary for survival under different types of cell wall stress. Wsc1p-GFP was concentrated in patches at the cell tips, it interacted with the Rho-GEF Rgf2p, and its overexpression activated cell wall biosynthesis. Our results are consistent with the notion that cell wall assembly is regulated by two different networks involving Rho1p. One includes signaling from Mtl2p through Rho1p to Pck1p, while the second one implicates signaling from Wsc1p and Rgf2p through Rho1p to activate glucan synthase (GS). Finally, signaling through the mitogen-activated protein kinase (MAPK) Pmk1p remained active in mtl2Δ and wsc1Δ disruptants exposed to cell wall stress, suggesting that the cell wall stress-sensing spectrum of Schizosaccharomyces pombe sensor-like proteins differs from that of Saccharomyces cerevisiae.S. Cruz was supported by a fellowship from BSCH (Banco Santander Central Hispano), P. García was supported by a fellowship from the Junta de Castilla y León, and S. Muñoz acknowledges support from a fellowship JAE-PreDoc granted by the CSIC, Spain. This work was supported by grants BFU2008-00963/BMC and BFU2011-24683/BMC from the CICYT, Spain and GR231 from the Junta de Castilla y León. IBFG acknowledges the institutional support granted by the Ramón Areces Foundation during 2011-2012.Peer ReviewedJohn Wiley & SonsBanco SantanderJunta de Castilla y LeónConsejo Superior de Investigaciones Científicas (España)Comisión Interministerial de Ciencia y Tecnología, CICYT (España)Fundación Ramón ArecesConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2015201520132015info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/116085reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1002/mbo3.113Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1160852026-05-22T06:33:51Z
dc.title.none.fl_str_mv The fission yeast cell wall stress sensor-like proteins Mtl2 and Wsc1 act by turning on the GTPase Rho1p but act independently of the cell wall integrity pathway
title The fission yeast cell wall stress sensor-like proteins Mtl2 and Wsc1 act by turning on the GTPase Rho1p but act independently of the cell wall integrity pathway
spellingShingle The fission yeast cell wall stress sensor-like proteins Mtl2 and Wsc1 act by turning on the GTPase Rho1p but act independently of the cell wall integrity pathway
Cruz, Sandra
Fission yeast
MAPK pathway
Cell wall sensor
Cell walls
title_short The fission yeast cell wall stress sensor-like proteins Mtl2 and Wsc1 act by turning on the GTPase Rho1p but act independently of the cell wall integrity pathway
title_full The fission yeast cell wall stress sensor-like proteins Mtl2 and Wsc1 act by turning on the GTPase Rho1p but act independently of the cell wall integrity pathway
title_fullStr The fission yeast cell wall stress sensor-like proteins Mtl2 and Wsc1 act by turning on the GTPase Rho1p but act independently of the cell wall integrity pathway
title_full_unstemmed The fission yeast cell wall stress sensor-like proteins Mtl2 and Wsc1 act by turning on the GTPase Rho1p but act independently of the cell wall integrity pathway
title_sort The fission yeast cell wall stress sensor-like proteins Mtl2 and Wsc1 act by turning on the GTPase Rho1p but act independently of the cell wall integrity pathway
dc.creator.none.fl_str_mv Cruz, Sandra
Muñoz Félix, Sofía
Manjón, Elvira
García, Patricia
Sánchez, Yolanda
author Cruz, Sandra
author_facet Cruz, Sandra
Muñoz Félix, Sofía
Manjón, Elvira
García, Patricia
Sánchez, Yolanda
author_role author
author2 Muñoz Félix, Sofía
Manjón, Elvira
García, Patricia
Sánchez, Yolanda
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Banco Santander
Junta de Castilla y León
Consejo Superior de Investigaciones Científicas (España)
Comisión Interministerial de Ciencia y Tecnología, CICYT (España)
Fundación Ramón Areces
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Fission yeast
MAPK pathway
Cell wall sensor
Cell walls
topic Fission yeast
MAPK pathway
Cell wall sensor
Cell walls
description This is an open access article under the terms of the Creative Commons Attribution License.
publishDate 2013
dc.date.none.fl_str_mv 2013
2015
2015
2015
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/116085
url http://hdl.handle.net/10261/116085
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1002/mbo3.113
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv John Wiley & Sons
publisher.none.fl_str_mv John Wiley & Sons
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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