The respiratory microbiome in bronchial mucosa and secretions from severe IgE-mediated asthma patients

Background: The bronchial microbiome in chronic lung diseases presents an abnormal pattern, but its microbial composition and regional differences in severe asthma have not been sufficiently addressed. The aim of the study was to describe the bacterial community in bronchial mucosa and secretions of...

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Autores: Millares L, Bermudo G, Pérez-Brocal V, Domingo C, Garcia-Nuñez M, Pomares X, Moya A, Monsó E
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2017
País:España
Institución:Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana (FISABIO)
Repositorio:r-FISABIO. Repositorio Institucional de Producción Científica
OAI Identifier:oai:fisabio.fundanetsuite.com:p1606
Acceso en línea:https://fisabio.portalinvestigacion.com/publicaciones/1606
Access Level:acceso abierto
Palabra clave:Microbiome
Asthma
Biopsy
Bronchial aspirate
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spelling The respiratory microbiome in bronchial mucosa and secretions from severe IgE-mediated asthma patientsMillares LBermudo GPérez-Brocal VDomingo CGarcia-Nuñez MPomares XMoya AMonsó EMicrobiomeAsthmaBiopsyBronchial aspirateBackground: The bronchial microbiome in chronic lung diseases presents an abnormal pattern, but its microbial composition and regional differences in severe asthma have not been sufficiently addressed. The aim of the study was to describe the bacterial community in bronchial mucosa and secretions of patients with severe chronic asthma chronically treated with corticosteroids in addition to usual care according to Global Initiative for Asthma. Bacterial community composition was obtained by 16S rRNA gene amplification and sequencing, and functional capabilities through PICRUSt. Results: Thirteen patients with severe asthma were included and provided 11 bronchial biopsies (BB) and 12 bronchial aspirates (BA) suitable for sequence analyses. Bacteroidetes, Firmicutes, Proteobacteria and Actinobacteria showed relative abundances (RAs) over 5% in BB, a cutoff that was reached by Streptococcus and Prevotella at genus level. Legionella genus attained a median RA of 2.7 (interquartile range 1.1-4.7) in BB samples. In BA a higher RA of Fusobacteria was found, when compared with BB [8.7 (5.9-11.4) vs 4.2 (0.8-7.5), p = 0.037], while the RA of Proteobacteria was lower in BA [4.3 (3.7-6.5) vs 17.1 (11.2-33.4), p = 0.005]. RA of the Legionella genus was also significantly lower in BA [0.004 (0.001-0.02) vs. 2.7 (1.1-4.7), p = 0.005]. Beta-diversity analysis confirmed the differences between the microbial communities in BA and BB (R-2 = 0.20, p = 0.001, Adonis test), and functional analysis revealed also statistically significant differences between both types of sample on Metabolism, Cellular processes, Human diseases, Organismal systems and Genetic information processing pathways. Conclusions: The microbiota in the bronchial mucosa of severe asthma has a specific pattern that is not accurately represented in bronchial secretions, which must be considered a different niche of bacteria growth.BMC2017info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://fisabio.portalinvestigacion.com/publicaciones/1606BMC MICROBIOLOGYISSN: 14712180reponame:r-FISABIO. Repositorio Institucional de Producción Científicainstname:Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana (FISABIO)Inglésinfo:eu-repo/semantics/openAccessoai:fisabio.fundanetsuite.com:p16062026-06-11T12:45:17Z
dc.title.none.fl_str_mv The respiratory microbiome in bronchial mucosa and secretions from severe IgE-mediated asthma patients
title The respiratory microbiome in bronchial mucosa and secretions from severe IgE-mediated asthma patients
spellingShingle The respiratory microbiome in bronchial mucosa and secretions from severe IgE-mediated asthma patients
Millares L
Microbiome
Asthma
Biopsy
Bronchial aspirate
title_short The respiratory microbiome in bronchial mucosa and secretions from severe IgE-mediated asthma patients
title_full The respiratory microbiome in bronchial mucosa and secretions from severe IgE-mediated asthma patients
title_fullStr The respiratory microbiome in bronchial mucosa and secretions from severe IgE-mediated asthma patients
title_full_unstemmed The respiratory microbiome in bronchial mucosa and secretions from severe IgE-mediated asthma patients
title_sort The respiratory microbiome in bronchial mucosa and secretions from severe IgE-mediated asthma patients
dc.creator.none.fl_str_mv Millares L
Bermudo G
Pérez-Brocal V
Domingo C
Garcia-Nuñez M
Pomares X
Moya A
Monsó E
author Millares L
author_facet Millares L
Bermudo G
Pérez-Brocal V
Domingo C
Garcia-Nuñez M
Pomares X
Moya A
Monsó E
author_role author
author2 Bermudo G
Pérez-Brocal V
Domingo C
Garcia-Nuñez M
Pomares X
Moya A
Monsó E
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Microbiome
Asthma
Biopsy
Bronchial aspirate
topic Microbiome
Asthma
Biopsy
Bronchial aspirate
description Background: The bronchial microbiome in chronic lung diseases presents an abnormal pattern, but its microbial composition and regional differences in severe asthma have not been sufficiently addressed. The aim of the study was to describe the bacterial community in bronchial mucosa and secretions of patients with severe chronic asthma chronically treated with corticosteroids in addition to usual care according to Global Initiative for Asthma. Bacterial community composition was obtained by 16S rRNA gene amplification and sequencing, and functional capabilities through PICRUSt. Results: Thirteen patients with severe asthma were included and provided 11 bronchial biopsies (BB) and 12 bronchial aspirates (BA) suitable for sequence analyses. Bacteroidetes, Firmicutes, Proteobacteria and Actinobacteria showed relative abundances (RAs) over 5% in BB, a cutoff that was reached by Streptococcus and Prevotella at genus level. Legionella genus attained a median RA of 2.7 (interquartile range 1.1-4.7) in BB samples. In BA a higher RA of Fusobacteria was found, when compared with BB [8.7 (5.9-11.4) vs 4.2 (0.8-7.5), p = 0.037], while the RA of Proteobacteria was lower in BA [4.3 (3.7-6.5) vs 17.1 (11.2-33.4), p = 0.005]. RA of the Legionella genus was also significantly lower in BA [0.004 (0.001-0.02) vs. 2.7 (1.1-4.7), p = 0.005]. Beta-diversity analysis confirmed the differences between the microbial communities in BA and BB (R-2 = 0.20, p = 0.001, Adonis test), and functional analysis revealed also statistically significant differences between both types of sample on Metabolism, Cellular processes, Human diseases, Organismal systems and Genetic information processing pathways. Conclusions: The microbiota in the bronchial mucosa of severe asthma has a specific pattern that is not accurately represented in bronchial secretions, which must be considered a different niche of bacteria growth.
publishDate 2017
dc.date.none.fl_str_mv 2017
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://fisabio.portalinvestigacion.com/publicaciones/1606
url https://fisabio.portalinvestigacion.com/publicaciones/1606
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv BMC
publisher.none.fl_str_mv BMC
dc.source.none.fl_str_mv BMC MICROBIOLOGY
ISSN: 14712180
reponame:r-FISABIO. Repositorio Institucional de Producción Científica
instname:Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana (FISABIO)
instname_str Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana (FISABIO)
reponame_str r-FISABIO. Repositorio Institucional de Producción Científica
collection r-FISABIO. Repositorio Institucional de Producción Científica
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