Treatment with integrase inhibitors alters SARS-CoV-2 neutralization levels measured with HIV-based pseudotypes in people living with HIV

The presence of neutralizing antibodies (NAbs) is a major correlate of protection for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. Thus, different in vitro pseudoviruses-based assays have been described to detect NAbs against SARS-CoV-2. However, the determination of NAbs...

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Autores: Torre Tarazona, Humberto Erick de la, González Robles, Alba, Cascajero, Almudena, Jiménez, Paloma, Miró Meda, José M. (José María), 1956-, Sánchez-Palomino, Sonsoles, Alcamí, José, Buzón, María J., García Pérez, Javier, Hospital Clinic HIV Investigators
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/196118
Acceso en línea:https://hdl.handle.net/2445/196118
Access Level:acceso abierto
Palabra clave:SARS-CoV-2
Inhibidors de la integrasa
VIH (Virus)
Neutralitat
Persones seropositives
Integrase inhibitors
HIV (Viruses)
Neutrality
HIV-positive persons
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spelling Treatment with integrase inhibitors alters SARS-CoV-2 neutralization levels measured with HIV-based pseudotypes in people living with HIVTorre Tarazona, Humberto Erick de laGonzález Robles, AlbaCascajero, AlmudenaJiménez, PalomaMiró Meda, José M. (José María), 1956-Sánchez-Palomino, SonsolesAlcamí, JoséBuzón, María J.García Pérez, JavierHospital Clinic HIV InvestigatorsSARS-CoV-2Inhibidors de la integrasaVIH (Virus)NeutralitatPersones seropositivesSARS-CoV-2Integrase inhibitorsHIV (Viruses)NeutralityHIV-positive personsThe presence of neutralizing antibodies (NAbs) is a major correlate of protection for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. Thus, different in vitro pseudoviruses-based assays have been described to detect NAbs against SARS-CoV-2. However, the determination of NAbs against SARS-CoV-2 in people living with HIV (PLWH) through HIV-based pseudoparticles could be influenced by cross-neutralization activity or treatment, impeding accurate titration of NAbs. Two assays were compared using replication-defective HIV or VSV-based particles pseudotyped with SARS-CoV-2 spike to measure NAbs in COVID-19-recovered and COVID-19-naïve PLWH. The assay based on HIV-pseudoparticles displayed neutralization activity in all COVID-19-recovered PLWH with a median neutralizing titer 50 (NT50) of 1417.0 (interquartile range [IQR]: 450.3-3284.0), but also in 67% of COVID-19-naïve PLWH (NT50: 631.5, IQR: 16.0-1535.0). Regarding VSV-pseudoparticles system, no neutralization was observed in COVID-19-naïve PLWH as expected, whereas in comparison with HIV-pseudoparticles assay lower neutralization titers were measured in 75% COVID-19-recovered PLWH (NT50: 100.5; IQR: 20.5-1353.0). Treatment with integrase inhibitors was associated with inaccurate increase in neutralization titers when HIV-based pseudoparticles were used. IgG purification and consequent elimination of drugs from samples avoided the interference with retroviral cycle and corrected the lack of specificity observed in HIV-pseudotyped assay. This study shows methodological alternatives based on pseudoviruses systems to determine specific SARS-CoV-2 neutralization titers in PLWH.Wiley2023202320232023info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion8 p.application/pdfhttps://hdl.handle.net/2445/196118Articles publicats en revistes (Medicina)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: https://doi.org/10.1002/jmv.28543Journal of Medical Virology, 2023, vol. 95, num. 2https://doi.org/10.1002/jmv.28543cc-by (c) Torre Tarazona, Erick de la et al., 2023http://creativecommons.org/licenses/by/3.0/es/info:eu-repo/semantics/openAccessoai:recercat.cat:2445/1961182026-05-29T05:05:01Z
dc.title.none.fl_str_mv Treatment with integrase inhibitors alters SARS-CoV-2 neutralization levels measured with HIV-based pseudotypes in people living with HIV
title Treatment with integrase inhibitors alters SARS-CoV-2 neutralization levels measured with HIV-based pseudotypes in people living with HIV
spellingShingle Treatment with integrase inhibitors alters SARS-CoV-2 neutralization levels measured with HIV-based pseudotypes in people living with HIV
Torre Tarazona, Humberto Erick de la
SARS-CoV-2
Inhibidors de la integrasa
VIH (Virus)
Neutralitat
Persones seropositives
SARS-CoV-2
Integrase inhibitors
HIV (Viruses)
Neutrality
HIV-positive persons
title_short Treatment with integrase inhibitors alters SARS-CoV-2 neutralization levels measured with HIV-based pseudotypes in people living with HIV
title_full Treatment with integrase inhibitors alters SARS-CoV-2 neutralization levels measured with HIV-based pseudotypes in people living with HIV
title_fullStr Treatment with integrase inhibitors alters SARS-CoV-2 neutralization levels measured with HIV-based pseudotypes in people living with HIV
title_full_unstemmed Treatment with integrase inhibitors alters SARS-CoV-2 neutralization levels measured with HIV-based pseudotypes in people living with HIV
title_sort Treatment with integrase inhibitors alters SARS-CoV-2 neutralization levels measured with HIV-based pseudotypes in people living with HIV
dc.creator.none.fl_str_mv Torre Tarazona, Humberto Erick de la
González Robles, Alba
Cascajero, Almudena
Jiménez, Paloma
Miró Meda, José M. (José María), 1956-
Sánchez-Palomino, Sonsoles
Alcamí, José
Buzón, María J.
García Pérez, Javier
Hospital Clinic HIV Investigators
author Torre Tarazona, Humberto Erick de la
author_facet Torre Tarazona, Humberto Erick de la
González Robles, Alba
Cascajero, Almudena
Jiménez, Paloma
Miró Meda, José M. (José María), 1956-
Sánchez-Palomino, Sonsoles
Alcamí, José
Buzón, María J.
García Pérez, Javier
Hospital Clinic HIV Investigators
author_role author
author2 González Robles, Alba
Cascajero, Almudena
Jiménez, Paloma
Miró Meda, José M. (José María), 1956-
Sánchez-Palomino, Sonsoles
Alcamí, José
Buzón, María J.
García Pérez, Javier
Hospital Clinic HIV Investigators
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv SARS-CoV-2
Inhibidors de la integrasa
VIH (Virus)
Neutralitat
Persones seropositives
SARS-CoV-2
Integrase inhibitors
HIV (Viruses)
Neutrality
HIV-positive persons
topic SARS-CoV-2
Inhibidors de la integrasa
VIH (Virus)
Neutralitat
Persones seropositives
SARS-CoV-2
Integrase inhibitors
HIV (Viruses)
Neutrality
HIV-positive persons
description The presence of neutralizing antibodies (NAbs) is a major correlate of protection for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. Thus, different in vitro pseudoviruses-based assays have been described to detect NAbs against SARS-CoV-2. However, the determination of NAbs against SARS-CoV-2 in people living with HIV (PLWH) through HIV-based pseudoparticles could be influenced by cross-neutralization activity or treatment, impeding accurate titration of NAbs. Two assays were compared using replication-defective HIV or VSV-based particles pseudotyped with SARS-CoV-2 spike to measure NAbs in COVID-19-recovered and COVID-19-naïve PLWH. The assay based on HIV-pseudoparticles displayed neutralization activity in all COVID-19-recovered PLWH with a median neutralizing titer 50 (NT50) of 1417.0 (interquartile range [IQR]: 450.3-3284.0), but also in 67% of COVID-19-naïve PLWH (NT50: 631.5, IQR: 16.0-1535.0). Regarding VSV-pseudoparticles system, no neutralization was observed in COVID-19-naïve PLWH as expected, whereas in comparison with HIV-pseudoparticles assay lower neutralization titers were measured in 75% COVID-19-recovered PLWH (NT50: 100.5; IQR: 20.5-1353.0). Treatment with integrase inhibitors was associated with inaccurate increase in neutralization titers when HIV-based pseudoparticles were used. IgG purification and consequent elimination of drugs from samples avoided the interference with retroviral cycle and corrected the lack of specificity observed in HIV-pseudotyped assay. This study shows methodological alternatives based on pseudoviruses systems to determine specific SARS-CoV-2 neutralization titers in PLWH.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/196118
url https://hdl.handle.net/2445/196118
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1002/jmv.28543
Journal of Medical Virology, 2023, vol. 95, num. 2
https://doi.org/10.1002/jmv.28543
dc.rights.none.fl_str_mv cc-by (c) Torre Tarazona, Erick de la et al., 2023
http://creativecommons.org/licenses/by/3.0/es/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Torre Tarazona, Erick de la et al., 2023
http://creativecommons.org/licenses/by/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 8 p.
application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv Articles publicats en revistes (Medicina)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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