Replication data for: "Effect of grazing deprivation as compared to grazing restriction on hair and saliva cortisol levels in pregnant ewes"

In the first week of the experiment, hair from the left forelimb was shaved in all ewes. Later, the same surface-hair samples were obtained twice, in Week 5 (W5) , when ewes were in the first half of pregnancy and in Week 10 (W10). Samples were processed following a methanol-based extraction protoco...

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Detalhes bibliográficos
Autores: Parés, Ricard, Lopez-Bejar, Manel, Carbajal, Annaïs, Llonch, Pol, Manteca, Xavier
Formato: conjunto de datos
Fecha de publicación:2025
País:España
Recursos:Consorci de Serveis Universitaris de Catalunya (CSUC)
Repositorio:CORA.Repositori de Dades de Recerca
OAI Identifier:oai:dnet:cora.rdr____::38714431c987afa2e419a27469fb592d
Acesso em linha:https://doi.org/10.34810/DATA1901
Access Level:acceso abierto
Palavra-chave:Medicine, Health and Life Sciences
Other
Cortisol
Hair
Saliva
Grazing
Pel
Ovella
Pastura
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repository_id_str
spelling Replication data for: "Effect of grazing deprivation as compared to grazing restriction on hair and saliva cortisol levels in pregnant ewes"Parés, RicardLopez-Bejar, ManelCarbajal, AnnaïsLlonch, PolManteca, XavierMedicine, Health and Life SciencesOtherCortisolHairSalivaGrazingPelOvellaPasturaIn the first week of the experiment, hair from the left forelimb was shaved in all ewes. Later, the same surface-hair samples were obtained twice, in Week 5 (W5) , when ewes were in the first half of pregnancy and in Week 10 (W10). Samples were processed following a methanol-based extraction protocol previously described (Davenport et al., 2006, Tallo-Parra et al., 2015). Cortisol concentrations in hair samples were determined by using a competitive cortisol enzyme immunoassay (EIA) kit. Saliva samples were taken in all ewes on Weeks 0, 3, 5, 8 and 10 (W0, W3, W5, W8, W10), and sampling was always performed between 16:00 and 17:30. Samples were obtained from a sponge of Salivette® tubes (Sarstedt, Aktiengesellsschaft & Co.; Nümbrecht, Germany). Samples were subsequently centrifuged at 3000 rpm for 10 minutes and then stored at -200C until analysis. Measurement of cortisol concentrations in saliva samples and the assay validation tests were performed using a commercial Enzyme-Linked ImmunoSorbentAssay (ELISA) kit (Neogen Corporation©; Ayr, UK). The assay was validated for the analysis of cortisol in saliva of Ovis aries following the criteria for an immunological validation (Midgley et al., 1969; Reimers & Lamb, 1991). The dilution test showed an R2 = 98.54 % and a mean recovery rate of 105.90% suggesting high specificity of the test.CORA.Repositori de Dades de RecercaParés Casanova, Ricard2025info:eu-repo/semantics/datasethttps://doi.org/10.34810/DATA1901reponame:CORA.Repositori de Dades de Recercainstname:Consorci de Serveis Universitaris de Catalunya (CSUC)Inglésinfo:eu-repo/semantics/openAccessCC0 1.0oai:dnet:cora.rdr____::38714431c987afa2e419a27469fb592d2026-06-17T12:20:17Z
dc.title.none.fl_str_mv Replication data for: "Effect of grazing deprivation as compared to grazing restriction on hair and saliva cortisol levels in pregnant ewes"
title Replication data for: "Effect of grazing deprivation as compared to grazing restriction on hair and saliva cortisol levels in pregnant ewes"
spellingShingle Replication data for: "Effect of grazing deprivation as compared to grazing restriction on hair and saliva cortisol levels in pregnant ewes"
Parés, Ricard
Medicine, Health and Life Sciences
Other
Cortisol
Hair
Saliva
Grazing
Pel
Ovella
Pastura
title_short Replication data for: "Effect of grazing deprivation as compared to grazing restriction on hair and saliva cortisol levels in pregnant ewes"
title_full Replication data for: "Effect of grazing deprivation as compared to grazing restriction on hair and saliva cortisol levels in pregnant ewes"
title_fullStr Replication data for: "Effect of grazing deprivation as compared to grazing restriction on hair and saliva cortisol levels in pregnant ewes"
title_full_unstemmed Replication data for: "Effect of grazing deprivation as compared to grazing restriction on hair and saliva cortisol levels in pregnant ewes"
title_sort Replication data for: "Effect of grazing deprivation as compared to grazing restriction on hair and saliva cortisol levels in pregnant ewes"
dc.creator.none.fl_str_mv Parés, Ricard
Lopez-Bejar, Manel
Carbajal, Annaïs
Llonch, Pol
Manteca, Xavier
author Parés, Ricard
author_facet Parés, Ricard
Lopez-Bejar, Manel
Carbajal, Annaïs
Llonch, Pol
Manteca, Xavier
author_role author
author2 Lopez-Bejar, Manel
Carbajal, Annaïs
Llonch, Pol
Manteca, Xavier
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Parés Casanova, Ricard
dc.subject.none.fl_str_mv Medicine, Health and Life Sciences
Other
Cortisol
Hair
Saliva
Grazing
Pel
Ovella
Pastura
topic Medicine, Health and Life Sciences
Other
Cortisol
Hair
Saliva
Grazing
Pel
Ovella
Pastura
description In the first week of the experiment, hair from the left forelimb was shaved in all ewes. Later, the same surface-hair samples were obtained twice, in Week 5 (W5) , when ewes were in the first half of pregnancy and in Week 10 (W10). Samples were processed following a methanol-based extraction protocol previously described (Davenport et al., 2006, Tallo-Parra et al., 2015). Cortisol concentrations in hair samples were determined by using a competitive cortisol enzyme immunoassay (EIA) kit. Saliva samples were taken in all ewes on Weeks 0, 3, 5, 8 and 10 (W0, W3, W5, W8, W10), and sampling was always performed between 16:00 and 17:30. Samples were obtained from a sponge of Salivette® tubes (Sarstedt, Aktiengesellsschaft & Co.; Nümbrecht, Germany). Samples were subsequently centrifuged at 3000 rpm for 10 minutes and then stored at -200C until analysis. Measurement of cortisol concentrations in saliva samples and the assay validation tests were performed using a commercial Enzyme-Linked ImmunoSorbentAssay (ELISA) kit (Neogen Corporation©; Ayr, UK). The assay was validated for the analysis of cortisol in saliva of Ovis aries following the criteria for an immunological validation (Midgley et al., 1969; Reimers & Lamb, 1991). The dilution test showed an R2 = 98.54 % and a mean recovery rate of 105.90% suggesting high specificity of the test.
publishDate 2025
dc.date.none.fl_str_mv 2025
dc.type.none.fl_str_mv info:eu-repo/semantics/dataset
format dataset
dc.identifier.none.fl_str_mv https://doi.org/10.34810/DATA1901
url https://doi.org/10.34810/DATA1901
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
CC0 1.0
eu_rights_str_mv openAccess
rights_invalid_str_mv CC0 1.0
dc.publisher.none.fl_str_mv CORA.Repositori de Dades de Recerca
publisher.none.fl_str_mv CORA.Repositori de Dades de Recerca
dc.source.none.fl_str_mv reponame:CORA.Repositori de Dades de Recerca
instname:Consorci de Serveis Universitaris de Catalunya (CSUC)
instname_str Consorci de Serveis Universitaris de Catalunya (CSUC)
reponame_str CORA.Repositori de Dades de Recerca
collection CORA.Repositori de Dades de Recerca
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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