Delegating Sex: Differential Gene Expression in Stolonizing Syllids Uncovers the Hormonal Control of Reproduction
Stolonizationinsyllidannelids is auniquemodeof reproductionamonganimals.Duringthebreedingseason, a structure resemblingthe adult but containing only gametes, called stolon, is formed generally at the posterior end of the animal.When stolons mature, they detachfromthe adult andgametes are released in...
| Autores: | , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Fecha de publicación: | 2019 |
| País: | España |
| Institución: | Universidad Complutense de Madrid (UCM) |
| Repositorio: | Docta Complutense |
| Idioma: | inglés |
| OAI Identifier: | oai:docta.ucm.es:20.500.14352/13499 |
| Acceso en línea: | https://hdl.handle.net/20.500.14352/13499 |
| Access Level: | acceso abierto |
| Palabra clave: | 574.5 595.1 Transcriptomics stolonizing syllids reproduction hormonal control Biología marina Invertebrados 2401.17 Invertebrados |
| Sumario: | Stolonizationinsyllidannelids is auniquemodeof reproductionamonganimals.Duringthebreedingseason, a structure resemblingthe adult but containing only gametes, called stolon, is formed generally at the posterior end of the animal.When stolons mature, they detachfromthe adult andgametes are released intothewater column. Theprocess is synchronizedwithineachspecies, and it hasbeen reportedtobeunder environmental andendogenous control,probably via endocrine regulation. Tofurtherunderstandreproduction in syllids and to elucidate the molecular toolkit underlying stolonization, we generated Illumina RNA-seq data from different tissues of reproductive and nonreproductive individuals of Syllismagdalena and characterized gene expression during the stolonization process. Several genes involved in gametogenesis (ovochymase, vitellogenin, testis-specific serine/threonine-kinase), immune response (complement receptor 2), neuronal development (tyrosine-protein kinase Src42A), cell proliferation (alpha-1D adrenergic receptor), and steroid metabolism (hydroxysteroid dehydrogenase 2) were found differentially expressed in the different tissues and conditions analyzed. Inaddition,our findings suggest that severalneurohormones, suchasmethyl farnesoate,dopamine, andserotonin,might trigger stolon formation, the correct maturation of gametes and the detachment of stolonswhen gametogenesis ends. The process seems tobe under circadian control, as indicated by the expression patterns of r-opsins. Overall, our results shed light into the genes that orchestrate the onset of gamete formation and improve our understanding of howsome hormones, previously reported to be involved in reproduction and metamorphosis processes in other invertebrates, seem to also regulate reproduction via stolonization. |
|---|