mcr -Colistin Resistance Genes Mobilized by IncX4, IncHI2, and IncI2 Plasmids in Escherichia coli of Pigs and White Stork in Spain

Colistin has become the last-line antimicrobial for the treatment of multidrug resistant (MDR) Enterobacterales in human medicine. To date, several colistin resistance genes have been described. Of them mcr -1 is disseminated worldwide in Escherichia coli of human and animal origin. The aim of this...

Descripción completa

Detalles Bibliográficos
Autores: Migura-Garcia, Lourdes|||0000-0003-2935-928X, González-López, Juanjo|||0000-0003-2419-5909, Martínez-Urtaza, Jaime|||0000-0001-6219-0418, Aguirre Sánchez, José Roberto|||0000-0002-7378-0699, Moreno Mingorance, Albert|||0000-0002-3073-5046, Pérez de Rozas, Ana|||0000-0002-5728-7642, Höfle, Ursula, Ramiro, Y., Gonzalez-Escalona, Narjol|||0000-0003-4568-0022
Tipo de recurso: artículo
Fecha de publicación:2020
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:226236
Acceso en línea:https://ddd.uab.cat/record/226236
https://dx.doi.org/urn:doi:10.3389/fmicb.2019.03072
Access Level:acceso abierto
Palabra clave:Escherichia coli
Colistin
Mcr -plasmids
MinION nanopore
Pigs
Storks
Spain
id ES_c843a4c6f656d16ce97678b85a0433dd
oai_identifier_str oai:ddd.uab.cat:226236
network_acronym_str ES
network_name_str España
repository_id_str
spelling mcr -Colistin Resistance Genes Mobilized by IncX4, IncHI2, and IncI2 Plasmids in Escherichia coli of Pigs and White Stork in SpainMigura-Garcia, Lourdes|||0000-0003-2935-928XGonzález-López, Juanjo|||0000-0003-2419-5909Martínez-Urtaza, Jaime|||0000-0001-6219-0418Aguirre Sánchez, José Roberto|||0000-0002-7378-0699Moreno Mingorance, Albert|||0000-0002-3073-5046Pérez de Rozas, Ana|||0000-0002-5728-7642Höfle, UrsulaRamiro, Y.Gonzalez-Escalona, Narjol|||0000-0003-4568-0022Escherichia coliColistinMcr -plasmidsMinION nanoporePigsStorksSpainColistin has become the last-line antimicrobial for the treatment of multidrug resistant (MDR) Enterobacterales in human medicine. To date, several colistin resistance genes have been described. Of them mcr -1 is disseminated worldwide in Escherichia coli of human and animal origin. The aim of this study was to characterize mcr -mediated resistance plasmids from E. coli of animal origin in Spain. From our strain collection, 70 E. coli of pig origin collected between 2005 and 2014 (10 per year, except for years 2009-2010-2013) were randomly selected and screened for the presence of mcr -genes. Additionally, 20 E. coli isolated in 2011 from white storks (Ciconia ciconia) from the same urban household waste landfill associated colony were also included. Whole genome sequencing of mcr -positive isolates was carried out on a MiSeq (Illumina). Hybrid whole genome sequencing strategy combining nanopore and Illumina technologies were performed in a selection of isolates to close the genomes and plasmids and identify the presence of antimicrobial resistance genes. Minimum inhibitory concentration (MIC) was used to assess the susceptibility to colistin. Mating experiments were carried out to evaluate transferability of the mcr -genes. A total of 19 mcr -1 and one mcr -4 positive isolates were detected, 15 from pigs distributed during the study period, and five from storks collected in 2011. No other mcr -variants were found. The MICs for colistin ranged between 4 and. 22020-01-0120202020-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/226236https://dx.doi.org/urn:doi:10.3389/fmicb.2019.03072reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengAgencia Estatal de Investigación https://doi.org/10.13039/501100011033 RTI2018-095586-B-C22open accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:2262362026-06-06T12:50:31Z
dc.title.none.fl_str_mv mcr -Colistin Resistance Genes Mobilized by IncX4, IncHI2, and IncI2 Plasmids in Escherichia coli of Pigs and White Stork in Spain
title mcr -Colistin Resistance Genes Mobilized by IncX4, IncHI2, and IncI2 Plasmids in Escherichia coli of Pigs and White Stork in Spain
spellingShingle mcr -Colistin Resistance Genes Mobilized by IncX4, IncHI2, and IncI2 Plasmids in Escherichia coli of Pigs and White Stork in Spain
Migura-Garcia, Lourdes|||0000-0003-2935-928X
Escherichia coli
Colistin
Mcr -plasmids
MinION nanopore
Pigs
Storks
Spain
title_short mcr -Colistin Resistance Genes Mobilized by IncX4, IncHI2, and IncI2 Plasmids in Escherichia coli of Pigs and White Stork in Spain
title_full mcr -Colistin Resistance Genes Mobilized by IncX4, IncHI2, and IncI2 Plasmids in Escherichia coli of Pigs and White Stork in Spain
title_fullStr mcr -Colistin Resistance Genes Mobilized by IncX4, IncHI2, and IncI2 Plasmids in Escherichia coli of Pigs and White Stork in Spain
title_full_unstemmed mcr -Colistin Resistance Genes Mobilized by IncX4, IncHI2, and IncI2 Plasmids in Escherichia coli of Pigs and White Stork in Spain
title_sort mcr -Colistin Resistance Genes Mobilized by IncX4, IncHI2, and IncI2 Plasmids in Escherichia coli of Pigs and White Stork in Spain
dc.creator.none.fl_str_mv Migura-Garcia, Lourdes|||0000-0003-2935-928X
González-López, Juanjo|||0000-0003-2419-5909
Martínez-Urtaza, Jaime|||0000-0001-6219-0418
Aguirre Sánchez, José Roberto|||0000-0002-7378-0699
Moreno Mingorance, Albert|||0000-0002-3073-5046
Pérez de Rozas, Ana|||0000-0002-5728-7642
Höfle, Ursula
Ramiro, Y.
Gonzalez-Escalona, Narjol|||0000-0003-4568-0022
author Migura-Garcia, Lourdes|||0000-0003-2935-928X
author_facet Migura-Garcia, Lourdes|||0000-0003-2935-928X
González-López, Juanjo|||0000-0003-2419-5909
Martínez-Urtaza, Jaime|||0000-0001-6219-0418
Aguirre Sánchez, José Roberto|||0000-0002-7378-0699
Moreno Mingorance, Albert|||0000-0002-3073-5046
Pérez de Rozas, Ana|||0000-0002-5728-7642
Höfle, Ursula
Ramiro, Y.
Gonzalez-Escalona, Narjol|||0000-0003-4568-0022
author_role author
author2 González-López, Juanjo|||0000-0003-2419-5909
Martínez-Urtaza, Jaime|||0000-0001-6219-0418
Aguirre Sánchez, José Roberto|||0000-0002-7378-0699
Moreno Mingorance, Albert|||0000-0002-3073-5046
Pérez de Rozas, Ana|||0000-0002-5728-7642
Höfle, Ursula
Ramiro, Y.
Gonzalez-Escalona, Narjol|||0000-0003-4568-0022
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Escherichia coli
Colistin
Mcr -plasmids
MinION nanopore
Pigs
Storks
Spain
topic Escherichia coli
Colistin
Mcr -plasmids
MinION nanopore
Pigs
Storks
Spain
description Colistin has become the last-line antimicrobial for the treatment of multidrug resistant (MDR) Enterobacterales in human medicine. To date, several colistin resistance genes have been described. Of them mcr -1 is disseminated worldwide in Escherichia coli of human and animal origin. The aim of this study was to characterize mcr -mediated resistance plasmids from E. coli of animal origin in Spain. From our strain collection, 70 E. coli of pig origin collected between 2005 and 2014 (10 per year, except for years 2009-2010-2013) were randomly selected and screened for the presence of mcr -genes. Additionally, 20 E. coli isolated in 2011 from white storks (Ciconia ciconia) from the same urban household waste landfill associated colony were also included. Whole genome sequencing of mcr -positive isolates was carried out on a MiSeq (Illumina). Hybrid whole genome sequencing strategy combining nanopore and Illumina technologies were performed in a selection of isolates to close the genomes and plasmids and identify the presence of antimicrobial resistance genes. Minimum inhibitory concentration (MIC) was used to assess the susceptibility to colistin. Mating experiments were carried out to evaluate transferability of the mcr -genes. A total of 19 mcr -1 and one mcr -4 positive isolates were detected, 15 from pigs distributed during the study period, and five from storks collected in 2011. No other mcr -variants were found. The MICs for colistin ranged between 4 and.
publishDate 2020
dc.date.none.fl_str_mv 2
2020-01-01
2020
2020-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/226236
https://dx.doi.org/urn:doi:10.3389/fmicb.2019.03072
url https://ddd.uab.cat/record/226236
https://dx.doi.org/urn:doi:10.3389/fmicb.2019.03072
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 RTI2018-095586-B-C22
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869419270795952128
score 15,300724

Similares en LA Referencia