Untargeted Gut Metabolomics to Delve the Interplay between Selenium Supplementation and Gut Microbiota

Selenium (Se) is an essential trace element with important health roles due to the antioxidant properties of selenoproteins. To analyze the interplay between Se and gut microbiota, gut metabolomic profiles were determined in conventional (C) and microbiota depleted mice (Abx) after Se-supplementatio...

Full description

Bibliographic Details
Authors: Callejón Leblic, Belén, Selma Royo, Marta, Collado, María Carmen, Gómez Ariza, José Luis, Abril, Nieves, García Barrera, Tamara
Format: article
Status:Published version
Publication Date:2021
Country:España
Institution:Consejo Superior de Investigaciones Científicas (CSIC)
Repository:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/264004
Online Access:http://hdl.handle.net/10261/264004
https://api.elsevier.com/content/abstract/scopus_id/85119618198
Access Level:Open access
Keyword:Functional food
Gut metabolomics
Gut microbiota
Mass spectrometry
Selenium
Untargeted metabolomics
id ES_c82d0b0f647ca513d83df063df83cbb5
oai_identifier_str oai:digital.csic.es:10261/264004
network_acronym_str ES
network_name_str España
repository_id_str
spelling Untargeted Gut Metabolomics to Delve the Interplay between Selenium Supplementation and Gut MicrobiotaCallejón Leblic, BelénSelma Royo, MartaCollado, María CarmenGómez Ariza, José LuisAbril, NievesGarcía Barrera, TamaraFunctional foodGut metabolomicsGut microbiotaMass spectrometrySeleniumUntargeted metabolomicsSelenium (Se) is an essential trace element with important health roles due to the antioxidant properties of selenoproteins. To analyze the interplay between Se and gut microbiota, gut metabolomic profiles were determined in conventional (C) and microbiota depleted mice (Abx) after Se-supplementation (Abx-Se) by untargeted metabolomics, using an analytical multiplatform based on GC-MS and UHPLC-QTOF-MS (MassIVE ID MSV000087829). Gut microbiota profiling was performed by 16S rRNA gene amplicon sequencing. Significant differences in the levels of about 70% of the gut metabolites determined, including fatty acyls, glycerolipids, glycerophospholipids, and steroids, were found in Abx-Se compared to Abx, and only 30% were different between Abx-Se and C, suggesting an important effect of Se-supplementation on Abx mice metabolism. At genus level, the correlation analysis showed strong associations between metabolites and gut bacterial profiles. Likewise, higher abundance of Lactobacillus spp., a potentially beneficial genus enriched after Se-supplementation, was associated with higher levels of prenol lipids, phosphatidylglycerols (C-Se), steroids and diterpenoids (Abx-Se), and also with lower levels of fatty acids (Abx-Se). Thus, we observed a crucial interaction between Se intake-microbiota-metabolites, although further studies to clarify the specific mechanisms are needed. This is the first study about untargeted gut metabolomics after microbiota depletion and Se-supplementation.This work was supported by the projects PG2018-096608-B-C21 f from the Spanish Ministry of Science and innovation (MCIN). Generación del Conocimiento. MCIN/ AEI /10.13039/501100011033/ FEDER “Una manera de hacer Europa” and UHU-1256905 from the FEDER Andalusian Operative Program 2014–2020 (Ministry of Economy, Knowledge, Business and Universities, Regional Government of Andalusia, Spain). Authors would like to acknowledge the support from The Ramón Areces Foundation (ref CIVP19A5918). Authors are grateful to FEDER (European Community) for financial support, Grant UNHU13-1X10-1611. Funding for open access charge: Universidad de Huelva / CBUA.Peer reviewedACS PublicationsMinisterio de Ciencia e Innovación (España)European CommissionJunta de AndalucíaFundación Ramón ArecesUniversidad de Huelva0000-0002-6204-48640000-0001-7997-74440000-0001-8248-85610000-0002-8859-9550Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202220222021info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/264004https://api.elsevier.com/content/abstract/scopus_id/85119618198reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésJournal of proteome researchhttps://doi.org/10.1021/acs.jproteome.1c00411Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2640042026-05-22T06:33:51Z
dc.title.none.fl_str_mv Untargeted Gut Metabolomics to Delve the Interplay between Selenium Supplementation and Gut Microbiota
title Untargeted Gut Metabolomics to Delve the Interplay between Selenium Supplementation and Gut Microbiota
spellingShingle Untargeted Gut Metabolomics to Delve the Interplay between Selenium Supplementation and Gut Microbiota
Callejón Leblic, Belén
Functional food
Gut metabolomics
Gut microbiota
Mass spectrometry
Selenium
Untargeted metabolomics
title_short Untargeted Gut Metabolomics to Delve the Interplay between Selenium Supplementation and Gut Microbiota
title_full Untargeted Gut Metabolomics to Delve the Interplay between Selenium Supplementation and Gut Microbiota
title_fullStr Untargeted Gut Metabolomics to Delve the Interplay between Selenium Supplementation and Gut Microbiota
title_full_unstemmed Untargeted Gut Metabolomics to Delve the Interplay between Selenium Supplementation and Gut Microbiota
title_sort Untargeted Gut Metabolomics to Delve the Interplay between Selenium Supplementation and Gut Microbiota
dc.creator.none.fl_str_mv Callejón Leblic, Belén
Selma Royo, Marta
Collado, María Carmen
Gómez Ariza, José Luis
Abril, Nieves
García Barrera, Tamara
author Callejón Leblic, Belén
author_facet Callejón Leblic, Belén
Selma Royo, Marta
Collado, María Carmen
Gómez Ariza, José Luis
Abril, Nieves
García Barrera, Tamara
author_role author
author2 Selma Royo, Marta
Collado, María Carmen
Gómez Ariza, José Luis
Abril, Nieves
García Barrera, Tamara
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia e Innovación (España)
European Commission
Junta de Andalucía
Fundación Ramón Areces
Universidad de Huelva
0000-0002-6204-4864
0000-0001-7997-7444
0000-0001-8248-8561
0000-0002-8859-9550
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Functional food
Gut metabolomics
Gut microbiota
Mass spectrometry
Selenium
Untargeted metabolomics
topic Functional food
Gut metabolomics
Gut microbiota
Mass spectrometry
Selenium
Untargeted metabolomics
description Selenium (Se) is an essential trace element with important health roles due to the antioxidant properties of selenoproteins. To analyze the interplay between Se and gut microbiota, gut metabolomic profiles were determined in conventional (C) and microbiota depleted mice (Abx) after Se-supplementation (Abx-Se) by untargeted metabolomics, using an analytical multiplatform based on GC-MS and UHPLC-QTOF-MS (MassIVE ID MSV000087829). Gut microbiota profiling was performed by 16S rRNA gene amplicon sequencing. Significant differences in the levels of about 70% of the gut metabolites determined, including fatty acyls, glycerolipids, glycerophospholipids, and steroids, were found in Abx-Se compared to Abx, and only 30% were different between Abx-Se and C, suggesting an important effect of Se-supplementation on Abx mice metabolism. At genus level, the correlation analysis showed strong associations between metabolites and gut bacterial profiles. Likewise, higher abundance of Lactobacillus spp., a potentially beneficial genus enriched after Se-supplementation, was associated with higher levels of prenol lipids, phosphatidylglycerols (C-Se), steroids and diterpenoids (Abx-Se), and also with lower levels of fatty acids (Abx-Se). Thus, we observed a crucial interaction between Se intake-microbiota-metabolites, although further studies to clarify the specific mechanisms are needed. This is the first study about untargeted gut metabolomics after microbiota depletion and Se-supplementation.
publishDate 2021
dc.date.none.fl_str_mv 2021
2022
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/264004
https://api.elsevier.com/content/abstract/scopus_id/85119618198
url http://hdl.handle.net/10261/264004
https://api.elsevier.com/content/abstract/scopus_id/85119618198
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Journal of proteome research
https://doi.org/10.1021/acs.jproteome.1c00411
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv ACS Publications
publisher.none.fl_str_mv ACS Publications
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869419263415025664
score 15,811543

Similar Items