Phosphatase of regenerating liver-1 (PRL-1) regulates actin dynamics during immunological synapse assembly and t cell effector function

The regulatory role of most dual specific phosphatases during T cell activation remains unknown. Here, we have studied the expression and function of phosphatases of regenerating liver (PRLs: PRL-1, PRL-2, and PRL-3) during T cell activation, as well as, the dynamic delivery of PRL-1 to the Immunolo...

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Autores: Castro-Sánchez, Patricia, Reyes, Raquel, Cabañas, Carlos, Roda-Navarro, Pedro
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2018
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/181412
Acceso en línea:http://hdl.handle.net/10261/181412
Access Level:acceso abierto
Palabra clave:T cell immune response
Immunological synapse
Phosphatases of regenerating liver
Actin cytoskeleton
IL-2
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spelling Phosphatase of regenerating liver-1 (PRL-1) regulates actin dynamics during immunological synapse assembly and t cell effector functionCastro-Sánchez, PatriciaReyes, RaquelCabañas, CarlosRoda-Navarro, PedroT cell immune responseImmunological synapsePhosphatases of regenerating liverActin cytoskeletonIL-2The regulatory role of most dual specific phosphatases during T cell activation remains unknown. Here, we have studied the expression and function of phosphatases of regenerating liver (PRLs: PRL-1, PRL-2, and PRL-3) during T cell activation, as well as, the dynamic delivery of PRL-1 to the Immunological Synapse (IS). We found that T cell activation downregulates the expression of PRL-2, resulting in an increased PRL-1/PRL-2 ratio. PRL-1 redistributed at the IS in two stages: Initially, it was transiently accumulated at scanning membranes enriched in CD3 and actin, and at later times, it was delivered at the contact site from pericentriolar, CD3∂-containing, vesicles. Once at the established IS, PRL-1 distributed to LFA-1 and CD3ϵ sites. Remarkably, PRL-1 was found to regulate actin dynamics during IS assembly and the secretion of IL-2. Moreover, pharmacological inhibition of the catalytic activity of the three PRLs reduced the secretion of IL-2. These results provide evidence indicating a regulatory role of PRL-1 during IS assembly and highlight the involvement of PRLs in immune responses by mature T cells.Ministry of Economy and Competitiveness (MINECO) with research grants SAF2012-33218 and SAF2016-75656-P. The later from the Agencia Estatal de investigación cofounded by “fondo Europeo de desarrollo regional (FEDER)Ministerio de Economía y Competitividad (España)European CommissionConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2019201920182019info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/181412reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/SAF2016-75656-PSíinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1814122026-05-22T06:33:51Z
dc.title.none.fl_str_mv Phosphatase of regenerating liver-1 (PRL-1) regulates actin dynamics during immunological synapse assembly and t cell effector function
title Phosphatase of regenerating liver-1 (PRL-1) regulates actin dynamics during immunological synapse assembly and t cell effector function
spellingShingle Phosphatase of regenerating liver-1 (PRL-1) regulates actin dynamics during immunological synapse assembly and t cell effector function
Castro-Sánchez, Patricia
T cell immune response
Immunological synapse
Phosphatases of regenerating liver
Actin cytoskeleton
IL-2
title_short Phosphatase of regenerating liver-1 (PRL-1) regulates actin dynamics during immunological synapse assembly and t cell effector function
title_full Phosphatase of regenerating liver-1 (PRL-1) regulates actin dynamics during immunological synapse assembly and t cell effector function
title_fullStr Phosphatase of regenerating liver-1 (PRL-1) regulates actin dynamics during immunological synapse assembly and t cell effector function
title_full_unstemmed Phosphatase of regenerating liver-1 (PRL-1) regulates actin dynamics during immunological synapse assembly and t cell effector function
title_sort Phosphatase of regenerating liver-1 (PRL-1) regulates actin dynamics during immunological synapse assembly and t cell effector function
dc.creator.none.fl_str_mv Castro-Sánchez, Patricia
Reyes, Raquel
Cabañas, Carlos
Roda-Navarro, Pedro
author Castro-Sánchez, Patricia
author_facet Castro-Sánchez, Patricia
Reyes, Raquel
Cabañas, Carlos
Roda-Navarro, Pedro
author_role author
author2 Reyes, Raquel
Cabañas, Carlos
Roda-Navarro, Pedro
author2_role author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
European Commission
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv T cell immune response
Immunological synapse
Phosphatases of regenerating liver
Actin cytoskeleton
IL-2
topic T cell immune response
Immunological synapse
Phosphatases of regenerating liver
Actin cytoskeleton
IL-2
description The regulatory role of most dual specific phosphatases during T cell activation remains unknown. Here, we have studied the expression and function of phosphatases of regenerating liver (PRLs: PRL-1, PRL-2, and PRL-3) during T cell activation, as well as, the dynamic delivery of PRL-1 to the Immunological Synapse (IS). We found that T cell activation downregulates the expression of PRL-2, resulting in an increased PRL-1/PRL-2 ratio. PRL-1 redistributed at the IS in two stages: Initially, it was transiently accumulated at scanning membranes enriched in CD3 and actin, and at later times, it was delivered at the contact site from pericentriolar, CD3∂-containing, vesicles. Once at the established IS, PRL-1 distributed to LFA-1 and CD3ϵ sites. Remarkably, PRL-1 was found to regulate actin dynamics during IS assembly and the secretion of IL-2. Moreover, pharmacological inhibition of the catalytic activity of the three PRLs reduced the secretion of IL-2. These results provide evidence indicating a regulatory role of PRL-1 during IS assembly and highlight the involvement of PRLs in immune responses by mature T cells.
publishDate 2018
dc.date.none.fl_str_mv 2018
2019
2019
2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/181412
url http://hdl.handle.net/10261/181412
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/SAF2016-75656-P
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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