Novel polymorphic microsatellites for the red-legged partridge ( Alectoris rufa ) and cross-species amplification in Alectoris graeca

The red-legged partridge, Alectoris rufa, is an endemic species of the southwestern Mediterranean, and the most popular game bird in the Iberian Peninsula. A total of 27 microsatellite loci was isolated from an enriched genomic library of A. rufa. Six perfect GT microsatellites were characterized an...

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Autores: González, Elena G., Castilla, Aurora M., Zardoya, Rafael
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2005
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/115741
Acceso en línea:http://hdl.handle.net/10261/115741
Access Level:acceso abierto
Palabra clave:Game bird
Hybridization
Microsatellites
Red-legged partridge
Rock partridge
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spelling Novel polymorphic microsatellites for the red-legged partridge ( Alectoris rufa ) and cross-species amplification in Alectoris graecaGonzález, Elena G.Castilla, Aurora M.Zardoya, RafaelGame birdHybridizationMicrosatellitesRed-legged partridgeRock partridgeThe red-legged partridge, Alectoris rufa, is an endemic species of the southwestern Mediterranean, and the most popular game bird in the Iberian Peninsula. A total of 27 microsatellite loci was isolated from an enriched genomic library of A. rufa. Six perfect GT microsatellites were characterized and optimized in 45 individuals of A. rufa. All loci revealed high levels of polymorphism with a number of alleles that ranged from three to 13. Observed heterozygosity ranged from 0.2 to 0.6. Cross-species amplification showed that all loci were also polymorphic in rock partridge, Alectoris graeca. The new markers will be useful in determining hybridization between both species of Alectoris.E.G.G. is sponsored by a predoctoral fellowship of the Ministerio de Educación y Ciencia (MEC).A.M.C. is supported by a Ramón y Cajal contract from the Spanish National Science Foundation (CSIC-MEC).This work received financial support from a project of the MEC to R. Z. (REN 2001–1514/GLO).Peer reviewedJohn Wiley & SonsConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201520152005info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/115741reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1111/j.1471-8286.2005.00960.xSíinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1157412026-05-22T06:33:51Z
dc.title.none.fl_str_mv Novel polymorphic microsatellites for the red-legged partridge ( Alectoris rufa ) and cross-species amplification in Alectoris graeca
title Novel polymorphic microsatellites for the red-legged partridge ( Alectoris rufa ) and cross-species amplification in Alectoris graeca
spellingShingle Novel polymorphic microsatellites for the red-legged partridge ( Alectoris rufa ) and cross-species amplification in Alectoris graeca
González, Elena G.
Game bird
Hybridization
Microsatellites
Red-legged partridge
Rock partridge
title_short Novel polymorphic microsatellites for the red-legged partridge ( Alectoris rufa ) and cross-species amplification in Alectoris graeca
title_full Novel polymorphic microsatellites for the red-legged partridge ( Alectoris rufa ) and cross-species amplification in Alectoris graeca
title_fullStr Novel polymorphic microsatellites for the red-legged partridge ( Alectoris rufa ) and cross-species amplification in Alectoris graeca
title_full_unstemmed Novel polymorphic microsatellites for the red-legged partridge ( Alectoris rufa ) and cross-species amplification in Alectoris graeca
title_sort Novel polymorphic microsatellites for the red-legged partridge ( Alectoris rufa ) and cross-species amplification in Alectoris graeca
dc.creator.none.fl_str_mv González, Elena G.
Castilla, Aurora M.
Zardoya, Rafael
author González, Elena G.
author_facet González, Elena G.
Castilla, Aurora M.
Zardoya, Rafael
author_role author
author2 Castilla, Aurora M.
Zardoya, Rafael
author2_role author
author
dc.contributor.none.fl_str_mv Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Game bird
Hybridization
Microsatellites
Red-legged partridge
Rock partridge
topic Game bird
Hybridization
Microsatellites
Red-legged partridge
Rock partridge
description The red-legged partridge, Alectoris rufa, is an endemic species of the southwestern Mediterranean, and the most popular game bird in the Iberian Peninsula. A total of 27 microsatellite loci was isolated from an enriched genomic library of A. rufa. Six perfect GT microsatellites were characterized and optimized in 45 individuals of A. rufa. All loci revealed high levels of polymorphism with a number of alleles that ranged from three to 13. Observed heterozygosity ranged from 0.2 to 0.6. Cross-species amplification showed that all loci were also polymorphic in rock partridge, Alectoris graeca. The new markers will be useful in determining hybridization between both species of Alectoris.
publishDate 2005
dc.date.none.fl_str_mv 2005
2015
2015
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/115741
url http://hdl.handle.net/10261/115741
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1111/j.1471-8286.2005.00960.x
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv John Wiley & Sons
publisher.none.fl_str_mv John Wiley & Sons
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.81155

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