Extracellular matrix protein microarray-based biosensor with single cell resolution: Integrin profiling and characterization of cell-biomaterial interactions

In the search of biomaterials that promote cell adhesion, it is crucial to explore the integrin-substrate dynamic interactions given in a certain cell type to design successful biofunctionalization strategies. Here, we use a microarray platform for a thorough characterization of cell adhesion to a p...

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Autores: González Pujana, Ainhoa, Santos Vizcaíno, Edorta, García Hernando, Maite, Hernáez Estrada, Beatriz, Martínez de Pancorbo Gómez, María de los Angeles, Benito López, Fernando, Igartua Olaechea, Manuela, Basabe Desmonts, Lourdes, Hernández Martín, Rosa María
Tipo de recurso: artículo
Fecha de publicación:2019
País:España
Institución:Universidad del País Vasco
Repositorio:Addi. Archivo Digital para la Docencia y la Investigación
OAI Identifier:oai:addi.ehu.eus:10810/36386
Acceso en línea:http://hdl.handle.net/10810/36386
Access Level:acceso abierto
Palabra clave:integrin
ECM
cell therapy
biosensor
biomaterial
single cell array
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spelling Extracellular matrix protein microarray-based biosensor with single cell resolution: Integrin profiling and characterization of cell-biomaterial interactionsGonzález Pujana, AinhoaSantos Vizcaíno, EdortaGarcía Hernando, MaiteHernáez Estrada, BeatrizMartínez de Pancorbo Gómez, María de los AngelesBenito López, FernandoIgartua Olaechea, ManuelaBasabe Desmonts, LourdesHernández Martín, Rosa MaríaintegrinECMcell therapybiosensorbiomaterialsingle cell arrayIn the search of biomaterials that promote cell adhesion, it is crucial to explore the integrin-substrate dynamic interactions given in a certain cell type to design successful biofunctionalization strategies. Here, we use a microarray platform for a thorough characterization of cell adhesion to a particular substrate. A biosensor based on an array of 20 μm fibronectin circular isles was adapted to tissue culture treated plates to facilitate the performance of cell adhesion assays and the posterior affinity analyses. This sensitive analytical tool enables not only the evaluation of the cell adhesion kinetics, but also the integrin profiling and their contribution to cell attachment and adhesion strengthening via clustering. In particular, the biosensor was able to detect a significantly slower adhesion kinetics in fibroblasts, namely Baby Hamster Kidney Fibroblasts (BHK) and Human Dermal Fibroblasts (hDF), in comparison to other cell types such as C2C12 Mouse Myoblasts (C2C12) or Human Mesenchymal Stem Cells (hMSCa). When directly comparing hDF and hMSCa, the analysis determined that the differing kinetics were caused by a distinct integrin expression profile. Whereas β1 presenting integrins were the major responsible for hDF attachment, hMSCa adherence was importantly dependent on β1 but also on other integrin classes. Additionally, results revealed that concerning cell adhesion consolidation, in hMSCa, both αvβ3 and β1-subunit-presenting integrins contributed similarly; whereas in hDF, the latter played a more important role. Hence, our biosensor provided crucial information for the development of new cell-adhesive biomaterials, which are key in multiple biomedical fields including cell therapy or tissue engineering.SAF2017-82292-R and BIO2016- 80417-P (MINECO/AEI/FEDER, UE), ICTS “NANBIOSIS” (Drug Formulation Unit, U10) and the support from the Basque Country Government (Grupos Consolidados, No ref: IT907-16, IT127119). Basque Government (Department of Education, Universities and Research) for the PhD grant (PRE_2018_2_0133). University of the Basque Country for the PhD grant(PIF16/204). Basque Government for the PhD grant (PRE_2018_2_300).Elsevier201920192019info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10810/36386reponame:Addi. Archivo Digital para la Docencia y la Investigacióninstname:Universidad del País VascoInglésinfo:eu-repo/grantAgreement/MINECO/SAF2017-82292-R/info:eu-repo/grantAgreement/MINECO/BIO2016-80417-P/https://www.sciencedirect.com/science/article/pii/S0925400519311530info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/3.0/es/© 2019 Elsevieroai:addi.ehu.eus:10810/363862026-06-18T09:23:17Z
dc.title.none.fl_str_mv Extracellular matrix protein microarray-based biosensor with single cell resolution: Integrin profiling and characterization of cell-biomaterial interactions
title Extracellular matrix protein microarray-based biosensor with single cell resolution: Integrin profiling and characterization of cell-biomaterial interactions
spellingShingle Extracellular matrix protein microarray-based biosensor with single cell resolution: Integrin profiling and characterization of cell-biomaterial interactions
González Pujana, Ainhoa
integrin
ECM
cell therapy
biosensor
biomaterial
single cell array
title_short Extracellular matrix protein microarray-based biosensor with single cell resolution: Integrin profiling and characterization of cell-biomaterial interactions
title_full Extracellular matrix protein microarray-based biosensor with single cell resolution: Integrin profiling and characterization of cell-biomaterial interactions
title_fullStr Extracellular matrix protein microarray-based biosensor with single cell resolution: Integrin profiling and characterization of cell-biomaterial interactions
title_full_unstemmed Extracellular matrix protein microarray-based biosensor with single cell resolution: Integrin profiling and characterization of cell-biomaterial interactions
title_sort Extracellular matrix protein microarray-based biosensor with single cell resolution: Integrin profiling and characterization of cell-biomaterial interactions
dc.creator.none.fl_str_mv González Pujana, Ainhoa
Santos Vizcaíno, Edorta
García Hernando, Maite
Hernáez Estrada, Beatriz
Martínez de Pancorbo Gómez, María de los Angeles
Benito López, Fernando
Igartua Olaechea, Manuela
Basabe Desmonts, Lourdes
Hernández Martín, Rosa María
author González Pujana, Ainhoa
author_facet González Pujana, Ainhoa
Santos Vizcaíno, Edorta
García Hernando, Maite
Hernáez Estrada, Beatriz
Martínez de Pancorbo Gómez, María de los Angeles
Benito López, Fernando
Igartua Olaechea, Manuela
Basabe Desmonts, Lourdes
Hernández Martín, Rosa María
author_role author
author2 Santos Vizcaíno, Edorta
García Hernando, Maite
Hernáez Estrada, Beatriz
Martínez de Pancorbo Gómez, María de los Angeles
Benito López, Fernando
Igartua Olaechea, Manuela
Basabe Desmonts, Lourdes
Hernández Martín, Rosa María
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv integrin
ECM
cell therapy
biosensor
biomaterial
single cell array
topic integrin
ECM
cell therapy
biosensor
biomaterial
single cell array
description In the search of biomaterials that promote cell adhesion, it is crucial to explore the integrin-substrate dynamic interactions given in a certain cell type to design successful biofunctionalization strategies. Here, we use a microarray platform for a thorough characterization of cell adhesion to a particular substrate. A biosensor based on an array of 20 μm fibronectin circular isles was adapted to tissue culture treated plates to facilitate the performance of cell adhesion assays and the posterior affinity analyses. This sensitive analytical tool enables not only the evaluation of the cell adhesion kinetics, but also the integrin profiling and their contribution to cell attachment and adhesion strengthening via clustering. In particular, the biosensor was able to detect a significantly slower adhesion kinetics in fibroblasts, namely Baby Hamster Kidney Fibroblasts (BHK) and Human Dermal Fibroblasts (hDF), in comparison to other cell types such as C2C12 Mouse Myoblasts (C2C12) or Human Mesenchymal Stem Cells (hMSCa). When directly comparing hDF and hMSCa, the analysis determined that the differing kinetics were caused by a distinct integrin expression profile. Whereas β1 presenting integrins were the major responsible for hDF attachment, hMSCa adherence was importantly dependent on β1 but also on other integrin classes. Additionally, results revealed that concerning cell adhesion consolidation, in hMSCa, both αvβ3 and β1-subunit-presenting integrins contributed similarly; whereas in hDF, the latter played a more important role. Hence, our biosensor provided crucial information for the development of new cell-adhesive biomaterials, which are key in multiple biomedical fields including cell therapy or tissue engineering.
publishDate 2019
dc.date.none.fl_str_mv 2019
2019
2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10810/36386
url http://hdl.handle.net/10810/36386
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/MINECO/SAF2017-82292-R/
info:eu-repo/grantAgreement/MINECO/BIO2016-80417-P/
https://www.sciencedirect.com/science/article/pii/S0925400519311530
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
© 2019 Elsevier
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/3.0/es/
© 2019 Elsevier
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Addi. Archivo Digital para la Docencia y la Investigación
instname:Universidad del País Vasco
instname_str Universidad del País Vasco
reponame_str Addi. Archivo Digital para la Docencia y la Investigación
collection Addi. Archivo Digital para la Docencia y la Investigación
repository.name.fl_str_mv
repository.mail.fl_str_mv
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