Global transcriptional profiles of the copper responses in the cyanobacterium synechocystis sp. PCC 6803

Copper is an essential element involved in fundamental processes like respiration and photosynthesis. However, it becomes toxic at high concentration, which has forced organisms to control its cellular concentration. We have recently described a copper resistance system in the cyanobacterium Synecho...

Descripción completa

Detalles Bibliográficos
Autores: Giner Lamia, Joaquín, López Maury, Luis, Florencio Bellido, Francisco Javier
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2014
País:España
Institución:Universidad de Sevilla (US)
Repositorio:idUS. Depósito de Investigación de la Universidad de Sevilla
OAI Identifier:oai:idus.us.es:11441/55940
Acceso en línea:http://hdl.handle.net/11441/55940
https://doi.org/10.1371/journal.pone.0108912
Access Level:acceso abierto
Palabra clave:Bacterial Proteins
Copper
Cytochromes c6
DNA-Binding Proteins
Environmental Pollutants
Gene Expression Profiling
Gene Expression Regulation
Molecular Sequence Annotation
Plastocyanin
Reactive Oxygen Species
Synechocystis
Transcriptome
id ES_c7a9ada1c48e461c2c3fa03e9367d37c
oai_identifier_str oai:idus.us.es:11441/55940
network_acronym_str ES
network_name_str España
repository_id_str
spelling Global transcriptional profiles of the copper responses in the cyanobacterium synechocystis sp. PCC 6803Giner Lamia, JoaquínLópez Maury, LuisFlorencio Bellido, Francisco JavierBacterial ProteinsCopperCytochromes c6DNA-Binding ProteinsEnvironmental PollutantsGene Expression ProfilingGene Expression RegulationMolecular Sequence AnnotationPlastocyaninReactive Oxygen SpeciesSynechocystisTranscriptomeCopper is an essential element involved in fundamental processes like respiration and photosynthesis. However, it becomes toxic at high concentration, which has forced organisms to control its cellular concentration. We have recently described a copper resistance system in the cyanobacterium Synechocystis sp. PCC 6803, which is mediated by the two-component system, CopRS, a RND metal transport system, CopBAC and a protein of unknown function, CopM. Here, we report the transcriptional responses to copper additions at non-toxic (0.3 μM) and toxic concentrations (3 μM) in the wild type and in the copper sensitive copR mutant strain. While 0.3 μM copper slightly stimulated metabolism and promoted the exchange between cytochrome c6 and plastocyanin as soluble electron carriers, the addition of 3 μM copper catalyzed the formation of ROS, led to a general stress response and induced expression of Fe-S cluster biogenesis genes. According to this, a double mutant strain copRsufR, which expresses constitutively the sufBCDS ope ron, tolerated higher copper concentration than the copR mutant strain, suggesting that Fe-S clusters are direct targets of copper toxicity in Synechocystis. In addition we have also demonstrated that InrS, a nickel binding transcriptional repressor that belong to the CsoR family of transcriptional factor, was involved in heavy metal homeostasis, including copper, in Synechocystis. Finally, global gene expression analysis of the copR mutant strain suggested that CopRS only controls the expression of copMRS and copBAC operons in response to copper.Junta de Andalucía BIO–284Ministerio de Economía y Competitividad BFU2010– 15708Unión Europea FEDER E–CLPN09–001Public Library of ScienceBioquímica Vegetal y Biología Molecular2014info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttp://hdl.handle.net/11441/55940https://doi.org/10.1371/journal.pone.0108912reponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)InglésPlos One, 9 (9), e108912-1-e108912-16.BIO–284info:eu-repo/grantAgreement/MINECO/BFU2010–15708/FEDER E–CLPN09–001http://dx.doi.org/10.1371/journal.pone.0108912info:eu-repo/semantics/openAccessoai:idus.us.es:11441/559402026-06-17T12:51:07Z
dc.title.none.fl_str_mv Global transcriptional profiles of the copper responses in the cyanobacterium synechocystis sp. PCC 6803
title Global transcriptional profiles of the copper responses in the cyanobacterium synechocystis sp. PCC 6803
spellingShingle Global transcriptional profiles of the copper responses in the cyanobacterium synechocystis sp. PCC 6803
Giner Lamia, Joaquín
Bacterial Proteins
Copper
Cytochromes c6
DNA-Binding Proteins
Environmental Pollutants
Gene Expression Profiling
Gene Expression Regulation
Molecular Sequence Annotation
Plastocyanin
Reactive Oxygen Species
Synechocystis
Transcriptome
title_short Global transcriptional profiles of the copper responses in the cyanobacterium synechocystis sp. PCC 6803
title_full Global transcriptional profiles of the copper responses in the cyanobacterium synechocystis sp. PCC 6803
title_fullStr Global transcriptional profiles of the copper responses in the cyanobacterium synechocystis sp. PCC 6803
title_full_unstemmed Global transcriptional profiles of the copper responses in the cyanobacterium synechocystis sp. PCC 6803
title_sort Global transcriptional profiles of the copper responses in the cyanobacterium synechocystis sp. PCC 6803
dc.creator.none.fl_str_mv Giner Lamia, Joaquín
López Maury, Luis
Florencio Bellido, Francisco Javier
author Giner Lamia, Joaquín
author_facet Giner Lamia, Joaquín
López Maury, Luis
Florencio Bellido, Francisco Javier
author_role author
author2 López Maury, Luis
Florencio Bellido, Francisco Javier
author2_role author
author
dc.contributor.none.fl_str_mv Bioquímica Vegetal y Biología Molecular
dc.subject.none.fl_str_mv Bacterial Proteins
Copper
Cytochromes c6
DNA-Binding Proteins
Environmental Pollutants
Gene Expression Profiling
Gene Expression Regulation
Molecular Sequence Annotation
Plastocyanin
Reactive Oxygen Species
Synechocystis
Transcriptome
topic Bacterial Proteins
Copper
Cytochromes c6
DNA-Binding Proteins
Environmental Pollutants
Gene Expression Profiling
Gene Expression Regulation
Molecular Sequence Annotation
Plastocyanin
Reactive Oxygen Species
Synechocystis
Transcriptome
description Copper is an essential element involved in fundamental processes like respiration and photosynthesis. However, it becomes toxic at high concentration, which has forced organisms to control its cellular concentration. We have recently described a copper resistance system in the cyanobacterium Synechocystis sp. PCC 6803, which is mediated by the two-component system, CopRS, a RND metal transport system, CopBAC and a protein of unknown function, CopM. Here, we report the transcriptional responses to copper additions at non-toxic (0.3 μM) and toxic concentrations (3 μM) in the wild type and in the copper sensitive copR mutant strain. While 0.3 μM copper slightly stimulated metabolism and promoted the exchange between cytochrome c6 and plastocyanin as soluble electron carriers, the addition of 3 μM copper catalyzed the formation of ROS, led to a general stress response and induced expression of Fe-S cluster biogenesis genes. According to this, a double mutant strain copRsufR, which expresses constitutively the sufBCDS ope ron, tolerated higher copper concentration than the copR mutant strain, suggesting that Fe-S clusters are direct targets of copper toxicity in Synechocystis. In addition we have also demonstrated that InrS, a nickel binding transcriptional repressor that belong to the CsoR family of transcriptional factor, was involved in heavy metal homeostasis, including copper, in Synechocystis. Finally, global gene expression analysis of the copR mutant strain suggested that CopRS only controls the expression of copMRS and copBAC operons in response to copper.
publishDate 2014
dc.date.none.fl_str_mv 2014
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11441/55940
https://doi.org/10.1371/journal.pone.0108912
url http://hdl.handle.net/11441/55940
https://doi.org/10.1371/journal.pone.0108912
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Plos One, 9 (9), e108912-1-e108912-16.
BIO–284
info:eu-repo/grantAgreement/MINECO/BFU2010–15708/
FEDER E–CLPN09–001
http://dx.doi.org/10.1371/journal.pone.0108912
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:idUS. Depósito de Investigación de la Universidad de Sevilla
instname:Universidad de Sevilla (US)
instname_str Universidad de Sevilla (US)
reponame_str idUS. Depósito de Investigación de la Universidad de Sevilla
collection idUS. Depósito de Investigación de la Universidad de Sevilla
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869419197915725824
score 15,300724

Similares en LA Referencia