Carotenoid biosynthesis during tomato fruit development: regulatory role of 1-deoxy-D-xylulose 5-phosphate synthase

Plant isoprenoids represent a heterogeneous group of compounds which play essential roles not only in growth and development, but also in the interaction of plants with their environment. Higher plants contain two pathways for the biosynthesis of isoprenoids: the mevalonate pathway, located in the c...

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Autores: Lois Rojas, Luisa María, Rodríguez Concepción, Manuel, Gallego, Francisco, Campos Martínez, Narciso, Boronat i Margosa, Albert
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2000
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/225956
Acceso en línea:https://hdl.handle.net/2445/225956
Access Level:acceso abierto
Palabra clave:Biologia molecular vegetal
Metabòlits vegetals
Plant molecular biology
Plant metabolites
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spelling Carotenoid biosynthesis during tomato fruit development: regulatory role of 1-deoxy-D-xylulose 5-phosphate synthaseLois Rojas, Luisa MaríaRodríguez Concepción, ManuelGallego, FranciscoCampos Martínez, NarcisoBoronat i Margosa, AlbertBiologia molecular vegetalMetabòlits vegetalsPlant molecular biologyPlant metabolitesPlant isoprenoids represent a heterogeneous group of compounds which play essential roles not only in growth and development, but also in the interaction of plants with their environment. Higher plants contain two pathways for the biosynthesis of isoprenoids: the mevalonate pathway, located in the cytosol/endoplasmic reticulum, and the recently discovered mevalonate-independent pathway (Rohmer pathway), located in the plastids. In order to evaluate the function of the Rohmer pathway in the regulation of the synthesis of plastidial isoprenoids, we have isolated a tomato cDNA encoding 1-deoxy-D-xylulose 5-phosphate synthase (DXS), the first enzyme of the pathway. We demonstrate in vivo activity and plastid targeting of plant DXS. Expression analysis of the tomato DXS gene indicates developmental and organ-specific regulation of mRNA accumulation and a strong correlation with carotenoid synthesis during fruit development. 1-Deoxy-D-xylulose feeding experiments, together with expression analysis of DXS and PSY1 (encoding the fruit-specific isoform of phytoene synthase) in wild-type and yellow flesh mutant fruits, indicate that DXS catalyses the first potentially regulatory step in carotenoid biosynthesis during early fruit ripening. Our results change the current view that PSY1 is the only regulatory enzyme in tomato fruit carotenogenesis, and point towards a coordinated role of both DXS and PSY1 in the control of fruit carotenoid synthesis.John Wiley & Sons2026202620002026info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersion11 p.application/pdfhttps://hdl.handle.net/2445/225956Articles publicats en revistes (Bioquímica i Biomedicina Molecular)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésVersió postprint del document publicat a: https://doi.org/10.1046/j.1365-313x.2000.00764.xPlant Journal, 2000, vol. 22, num.6, p. 503-513https://doi.org/10.1046/j.1365-313x.2000.00764.x(c) John Wiley & Sons, 2000info:eu-repo/semantics/openAccessoai:recercat.cat:2445/2259562026-05-29T05:05:01Z
dc.title.none.fl_str_mv Carotenoid biosynthesis during tomato fruit development: regulatory role of 1-deoxy-D-xylulose 5-phosphate synthase
title Carotenoid biosynthesis during tomato fruit development: regulatory role of 1-deoxy-D-xylulose 5-phosphate synthase
spellingShingle Carotenoid biosynthesis during tomato fruit development: regulatory role of 1-deoxy-D-xylulose 5-phosphate synthase
Lois Rojas, Luisa María
Biologia molecular vegetal
Metabòlits vegetals
Plant molecular biology
Plant metabolites
title_short Carotenoid biosynthesis during tomato fruit development: regulatory role of 1-deoxy-D-xylulose 5-phosphate synthase
title_full Carotenoid biosynthesis during tomato fruit development: regulatory role of 1-deoxy-D-xylulose 5-phosphate synthase
title_fullStr Carotenoid biosynthesis during tomato fruit development: regulatory role of 1-deoxy-D-xylulose 5-phosphate synthase
title_full_unstemmed Carotenoid biosynthesis during tomato fruit development: regulatory role of 1-deoxy-D-xylulose 5-phosphate synthase
title_sort Carotenoid biosynthesis during tomato fruit development: regulatory role of 1-deoxy-D-xylulose 5-phosphate synthase
dc.creator.none.fl_str_mv Lois Rojas, Luisa María
Rodríguez Concepción, Manuel
Gallego, Francisco
Campos Martínez, Narciso
Boronat i Margosa, Albert
author Lois Rojas, Luisa María
author_facet Lois Rojas, Luisa María
Rodríguez Concepción, Manuel
Gallego, Francisco
Campos Martínez, Narciso
Boronat i Margosa, Albert
author_role author
author2 Rodríguez Concepción, Manuel
Gallego, Francisco
Campos Martínez, Narciso
Boronat i Margosa, Albert
author2_role author
author
author
author
dc.subject.none.fl_str_mv Biologia molecular vegetal
Metabòlits vegetals
Plant molecular biology
Plant metabolites
topic Biologia molecular vegetal
Metabòlits vegetals
Plant molecular biology
Plant metabolites
description Plant isoprenoids represent a heterogeneous group of compounds which play essential roles not only in growth and development, but also in the interaction of plants with their environment. Higher plants contain two pathways for the biosynthesis of isoprenoids: the mevalonate pathway, located in the cytosol/endoplasmic reticulum, and the recently discovered mevalonate-independent pathway (Rohmer pathway), located in the plastids. In order to evaluate the function of the Rohmer pathway in the regulation of the synthesis of plastidial isoprenoids, we have isolated a tomato cDNA encoding 1-deoxy-D-xylulose 5-phosphate synthase (DXS), the first enzyme of the pathway. We demonstrate in vivo activity and plastid targeting of plant DXS. Expression analysis of the tomato DXS gene indicates developmental and organ-specific regulation of mRNA accumulation and a strong correlation with carotenoid synthesis during fruit development. 1-Deoxy-D-xylulose feeding experiments, together with expression analysis of DXS and PSY1 (encoding the fruit-specific isoform of phytoene synthase) in wild-type and yellow flesh mutant fruits, indicate that DXS catalyses the first potentially regulatory step in carotenoid biosynthesis during early fruit ripening. Our results change the current view that PSY1 is the only regulatory enzyme in tomato fruit carotenogenesis, and point towards a coordinated role of both DXS and PSY1 in the control of fruit carotenoid synthesis.
publishDate 2000
dc.date.none.fl_str_mv 2000
2026
2026
2026
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/225956
url https://hdl.handle.net/2445/225956
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Versió postprint del document publicat a: https://doi.org/10.1046/j.1365-313x.2000.00764.x
Plant Journal, 2000, vol. 22, num.6, p. 503-513
https://doi.org/10.1046/j.1365-313x.2000.00764.x
dc.rights.none.fl_str_mv (c) John Wiley & Sons, 2000
info:eu-repo/semantics/openAccess
rights_invalid_str_mv (c) John Wiley & Sons, 2000
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 11 p.
application/pdf
dc.publisher.none.fl_str_mv John Wiley & Sons
publisher.none.fl_str_mv John Wiley & Sons
dc.source.none.fl_str_mv Articles publicats en revistes (Bioquímica i Biomedicina Molecular)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.812429

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