Cyclosporine A Decreases Dryness-Induced Hyperexcitability of Corneal Cold-Sensitive Nerve Terminals

Cyclosporine A (CsA) is used for the treatment of dry eye (DE) with good clinical results, improving tear secretion and decreasing subjective symptoms. These effects are attributed to the improved tear film dynamics, but there are no data on the effect of CsA on the abnormal sensory nerve activity c...

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Authors: Gyenes, Andrea, Tapasztó, Zsófia, Quirce, Susana, Luna, Carolina, Frutos-Rincón, Laura, Gallar, Juana, Acosta, M. Carmen, Kovacs, Illes
Format: article
Publication Date:2023
Country:España
Institution:Universidad Miguel Hernández de Elche
Repository:REDIUMH. Depósito Digital de la UMH
OAI Identifier:oai:dspace.umh.es:11000/38119
Online Access:https://hdl.handle.net/11000/38119
Access Level:Open access
Keyword:dry eye
cold thermoreceptors
cyclosporine A
corneal nerves
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spelling Cyclosporine A Decreases Dryness-Induced Hyperexcitability of Corneal Cold-Sensitive Nerve TerminalsGyenes, AndreaTapasztó, ZsófiaQuirce, SusanaLuna, CarolinaFrutos-Rincón, LauraGallar, JuanaAcosta, M. CarmenKovacs, Illesdry eyecold thermoreceptorscyclosporine Acorneal nervesCyclosporine A (CsA) is used for the treatment of dry eye (DE) with good clinical results, improving tear secretion and decreasing subjective symptoms. These effects are attributed to the improved tear film dynamics, but there are no data on the effect of CsA on the abnormal sensory nerve activity characteristic in DE. Our purpose was to evaluate the CsA effect on the enhanced activity of corneal cold thermoreceptors in a tear-deficient DE animal model using in vitro extracellular recording of cold thermoreceptors nerve terminal impulses (NTIs) before and in the presence of CsA. NTI shape was also analyzed. Blinking frequency and tearing rate were also measured in awake animals before and after topical CsA. CsA increased the tearing and blinking of treated animals. CsA significantly decreased the peak response to cold of cold thermoreceptors. Neither their spontaneous NTIs discharge rate nor their cooling threshold were modified. CsA also seemed to reverse some of the changes in NTI shape induced by tear deficiency. These data suggest that, at least in part, the beneficial clinical effects of CsA in DE can be attributed to a direct effect on sensory nerve endings, although the precise mechanisms underlying this effect need further studies to be fully clarified.MDPIDepartamentos de la UMH::FisiologíaInstitutos de la UMH::Instituto de Neurociencias202520252023info:eu-repo/semantics/articleapplication/pdf13application/pdfhttps://hdl.handle.net/11000/38119reponame:REDIUMH. Depósito Digital de la UMHinstname:Universidad Miguel Hernández de ElcheInglés10.3390/ijms241613025info:eu-repo/semantics/openAccessAttribution-NonCommercial-NoDerivatives 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/oai:dspace.umh.es:11000/381192026-05-27T13:36:21Z
dc.title.none.fl_str_mv Cyclosporine A Decreases Dryness-Induced Hyperexcitability of Corneal Cold-Sensitive Nerve Terminals
title Cyclosporine A Decreases Dryness-Induced Hyperexcitability of Corneal Cold-Sensitive Nerve Terminals
spellingShingle Cyclosporine A Decreases Dryness-Induced Hyperexcitability of Corneal Cold-Sensitive Nerve Terminals
Gyenes, Andrea
dry eye
cold thermoreceptors
cyclosporine A
corneal nerves
title_short Cyclosporine A Decreases Dryness-Induced Hyperexcitability of Corneal Cold-Sensitive Nerve Terminals
title_full Cyclosporine A Decreases Dryness-Induced Hyperexcitability of Corneal Cold-Sensitive Nerve Terminals
title_fullStr Cyclosporine A Decreases Dryness-Induced Hyperexcitability of Corneal Cold-Sensitive Nerve Terminals
title_full_unstemmed Cyclosporine A Decreases Dryness-Induced Hyperexcitability of Corneal Cold-Sensitive Nerve Terminals
title_sort Cyclosporine A Decreases Dryness-Induced Hyperexcitability of Corneal Cold-Sensitive Nerve Terminals
dc.creator.none.fl_str_mv Gyenes, Andrea
Tapasztó, Zsófia
Quirce, Susana
Luna, Carolina
Frutos-Rincón, Laura
Gallar, Juana
Acosta, M. Carmen
Kovacs, Illes
author Gyenes, Andrea
author_facet Gyenes, Andrea
Tapasztó, Zsófia
Quirce, Susana
Luna, Carolina
Frutos-Rincón, Laura
Gallar, Juana
Acosta, M. Carmen
Kovacs, Illes
author_role author
author2 Tapasztó, Zsófia
Quirce, Susana
Luna, Carolina
Frutos-Rincón, Laura
Gallar, Juana
Acosta, M. Carmen
Kovacs, Illes
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Departamentos de la UMH::Fisiología
Institutos de la UMH::Instituto de Neurociencias
dc.subject.none.fl_str_mv dry eye
cold thermoreceptors
cyclosporine A
corneal nerves
topic dry eye
cold thermoreceptors
cyclosporine A
corneal nerves
description Cyclosporine A (CsA) is used for the treatment of dry eye (DE) with good clinical results, improving tear secretion and decreasing subjective symptoms. These effects are attributed to the improved tear film dynamics, but there are no data on the effect of CsA on the abnormal sensory nerve activity characteristic in DE. Our purpose was to evaluate the CsA effect on the enhanced activity of corneal cold thermoreceptors in a tear-deficient DE animal model using in vitro extracellular recording of cold thermoreceptors nerve terminal impulses (NTIs) before and in the presence of CsA. NTI shape was also analyzed. Blinking frequency and tearing rate were also measured in awake animals before and after topical CsA. CsA increased the tearing and blinking of treated animals. CsA significantly decreased the peak response to cold of cold thermoreceptors. Neither their spontaneous NTIs discharge rate nor their cooling threshold were modified. CsA also seemed to reverse some of the changes in NTI shape induced by tear deficiency. These data suggest that, at least in part, the beneficial clinical effects of CsA in DE can be attributed to a direct effect on sensory nerve endings, although the precise mechanisms underlying this effect need further studies to be fully clarified.
publishDate 2023
dc.date.none.fl_str_mv 2023
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/11000/38119
url https://hdl.handle.net/11000/38119
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv 10.3390/ijms241613025
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.format.none.fl_str_mv application/pdf
13
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:REDIUMH. Depósito Digital de la UMH
instname:Universidad Miguel Hernández de Elche
instname_str Universidad Miguel Hernández de Elche
reponame_str REDIUMH. Depósito Digital de la UMH
collection REDIUMH. Depósito Digital de la UMH
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.811543

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