In vitro uptake and processing of maize auxin-binding proteins by ER-derived microsomes

We have analyzed auxin-binding proteins from maize encoded by the Zm-ERabp gene family. Open reading frames of cDNA clones predict proteins containing N-terminal hydrophobic signal sequences. In vitro studies show that the Zm-ERabpl protein can be translocated into ER-derived microsomes where it is...

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Detalles Bibliográficos
Autores: Campos Martínez, Narciso, Schell, Jeff, Palme, Klaus
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:1994
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/225950
Acceso en línea:https://hdl.handle.net/2445/225950
Access Level:acceso abierto
Palabra clave:Proteïnes vegetals
Biologia molecular vegetal
Plant proteins
Plant molecular biology
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spelling In vitro uptake and processing of maize auxin-binding proteins by ER-derived microsomesCampos Martínez, NarcisoSchell, JeffPalme, KlausProteïnes vegetalsBiologia molecular vegetalPlant proteinsPlant molecular biologyWe have analyzed auxin-binding proteins from maize encoded by the Zm-ERabp gene family. Open reading frames of cDNA clones predict proteins containing N-terminal hydrophobic signal sequences. In vitro studies show that the Zm-ERabpl protein can be translocated into ER-derived microsomes where it is processed and glycosylated. A cDNA clone encoding the Zm-ERabp4 protein predicts an open reading frame with a signal sequence that shows striking differences in charge distribution, in comparison to the signal sequence of Zm-ERabpl. Two translation products are synthesized from the Zm-ERabp4 transcript in the in vitro system, but only one of them is translocated into maize endosperm microsomes, indicating that specific cotranslational modifications in the primary sequence remaining after processing may play a role in the cellular trafficking of the Zm-ERabp4 protein.Japanese Society of Plant Physiologists.1994info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionapplication/pdfhttps://hdl.handle.net/2445/225950Articles publicats en revistes (Bioquímica i Biomedicina Molecular)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésVersió postprint del document publicat a: https://doi.org/10.1093/oxfordjournals.pcp.a078579Plant and Cell Physiology, 1994, vol. 35, num.2, p. 153-161https://doi.org/10.1093/oxfordjournals.pcp.a078579(c) Campos, N. et al., 1994info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/2259502026-05-27T06:46:51Z
dc.title.none.fl_str_mv In vitro uptake and processing of maize auxin-binding proteins by ER-derived microsomes
title In vitro uptake and processing of maize auxin-binding proteins by ER-derived microsomes
spellingShingle In vitro uptake and processing of maize auxin-binding proteins by ER-derived microsomes
Campos Martínez, Narciso
Proteïnes vegetals
Biologia molecular vegetal
Plant proteins
Plant molecular biology
title_short In vitro uptake and processing of maize auxin-binding proteins by ER-derived microsomes
title_full In vitro uptake and processing of maize auxin-binding proteins by ER-derived microsomes
title_fullStr In vitro uptake and processing of maize auxin-binding proteins by ER-derived microsomes
title_full_unstemmed In vitro uptake and processing of maize auxin-binding proteins by ER-derived microsomes
title_sort In vitro uptake and processing of maize auxin-binding proteins by ER-derived microsomes
dc.creator.none.fl_str_mv Campos Martínez, Narciso
Schell, Jeff
Palme, Klaus
author Campos Martínez, Narciso
author_facet Campos Martínez, Narciso
Schell, Jeff
Palme, Klaus
author_role author
author2 Schell, Jeff
Palme, Klaus
author2_role author
author
dc.subject.none.fl_str_mv Proteïnes vegetals
Biologia molecular vegetal
Plant proteins
Plant molecular biology
topic Proteïnes vegetals
Biologia molecular vegetal
Plant proteins
Plant molecular biology
description We have analyzed auxin-binding proteins from maize encoded by the Zm-ERabp gene family. Open reading frames of cDNA clones predict proteins containing N-terminal hydrophobic signal sequences. In vitro studies show that the Zm-ERabpl protein can be translocated into ER-derived microsomes where it is processed and glycosylated. A cDNA clone encoding the Zm-ERabp4 protein predicts an open reading frame with a signal sequence that shows striking differences in charge distribution, in comparison to the signal sequence of Zm-ERabpl. Two translation products are synthesized from the Zm-ERabp4 transcript in the in vitro system, but only one of them is translocated into maize endosperm microsomes, indicating that specific cotranslational modifications in the primary sequence remaining after processing may play a role in the cellular trafficking of the Zm-ERabp4 protein.
publishDate 1994
dc.date.none.fl_str_mv 1994
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/225950
url https://hdl.handle.net/2445/225950
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Versió postprint del document publicat a: https://doi.org/10.1093/oxfordjournals.pcp.a078579
Plant and Cell Physiology, 1994, vol. 35, num.2, p. 153-161
https://doi.org/10.1093/oxfordjournals.pcp.a078579
dc.rights.none.fl_str_mv (c) Campos, N. et al., 1994
info:eu-repo/semantics/openAccess
rights_invalid_str_mv (c) Campos, N. et al., 1994
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Japanese Society of Plant Physiologists.
publisher.none.fl_str_mv Japanese Society of Plant Physiologists.
dc.source.none.fl_str_mv Articles publicats en revistes (Bioquímica i Biomedicina Molecular)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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