The tree that hides the forest: Cryptic diversity and phylogenetic relationships in the Palaearctic vector Obsoletus/Scoticus Complex (Diptera: Ceratopogonidae) at the European level
Background: Culicoides obsoletus is an abundant and widely distributed Holarctic biting midge species, involved in the transmission of bluetongue virus (BTV) and Schmallenberg virus (SBV) to wild and domestic ruminants. Females of this vector species are often reported jointly with two morphological...
| Autores: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2020 |
| País: | España |
| Institución: | Universidad de Zaragoza |
| Repositorio: | Zaguán. Repositorio Digital de la Universidad de Zaragoza |
| OAI Identifier: | oai:zaguan.unizar.es:89741 |
| Acceso en línea: | http://zaguan.unizar.es/record/89741 |
| Access Level: | acceso abierto |
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The tree that hides the forest: Cryptic diversity and phylogenetic relationships in the Palaearctic vector Obsoletus/Scoticus Complex (Diptera: Ceratopogonidae) at the European levelMignotte, A.Garros, C.Gardès, L.Balenghien, T.Duhayon, M.Rakotoarivony, I.Tabourin, L.Poujol, L.Mathieu, B.Ibañez-Justicia, A.Deniz, A.Cvetkovikj, A.Purse, B.V.Ramilo, D.W.Stougiou, D.Werner, D.Pudar, D.Petric, D.Veronesi, E.Jacobs, F.Kampen, H.Pereira Da Fonseca, I.Lucientes, J.Navarro, J.De La Puente, J.M.Stefanovska, J.Searle, K.R.Khallaayoune, K.Culverwell, C.L.Larska, M.Bourquia, M.Goffredo, M.Bisia, M.England, M.Robin, M.Quaglia, M.Miranda-Chueca, M.Á.Bødker, R.Estrada-Penã, R.Carpenter, S.Tchakarova, S.Boutsini, S.Sviland, S.Schäfer, S.M.Ozolina¸, Z.Seglina¸, Z.Vatansever, Z.Huber, K.Background: Culicoides obsoletus is an abundant and widely distributed Holarctic biting midge species, involved in the transmission of bluetongue virus (BTV) and Schmallenberg virus (SBV) to wild and domestic ruminants. Females of this vector species are often reported jointly with two morphologically very close species, C. scoticus and C. montanus, forming the Obsoletus/Scoticus Complex. Recently, cryptic diversity within C. obsoletus was reported in geographically distant sites. Clear delineation of species and characterization of genetic variability is mandatory to revise their taxonomic status and assess the vector role of each taxonomic entity. Our objectives were to characterize and map the cryptic diversity within the Obsoletus/Scoticus Complex. Methods: Portion of the cox1 mitochondrial gene of 3763 individuals belonging to the Obsoletus/Scoticus Complex was sequenced. Populations from 20 countries along a Palaearctic Mediterranean transect covering Scandinavia to Canary islands (North to South) and Canary islands to Turkey (West to East) were included. Genetic diversity based on cox1 barcoding was supported by 16S rDNA mitochondrial gene sequences and a gene coding for ribosomal 28S rDNA. Species delimitation using a multi-marker methodology was used to revise the current taxonomic scheme of the Obsoletus/Scoticus Complex. Results: Our analysis showed the existence of three phylogenetic clades (C. obsoletus clade O2, C. obsoletus clade dark and one not yet named and identified) within C. obsoletus. These analyses also revealed two intra-specific clades within C. scoticus and raised questions about the taxonomic status of C. montanus. Conclusions: To our knowledge, our study provides the first genetic characterization of the Obsoletus/Scoticus Complex on a large geographical scale and allows a revision of the current taxonomic classification for an important group of vector species of livestock viruses in the Palaearctic region.[Figure not available: See fulltext.]2020info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://zaguan.unizar.es/record/89741reponame:Zaguán. Repositorio Digital de la Universidad de Zaragozainstname:Universidad de ZaragozaInglésinfo:eu-repo/grantAgreement/EC/ECDC/OC-EFSA-AHAW-2013-02-FWC1 VectorNetinfo:eu-repo/grantAgreement/EC/H2020/727393This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 727393-PALE-Bluinfo:eu-repo/semantics/openAccessoai:zaguan.unizar.es:897412026-05-29T13:59:51Z |
| dc.title.none.fl_str_mv |
The tree that hides the forest: Cryptic diversity and phylogenetic relationships in the Palaearctic vector Obsoletus/Scoticus Complex (Diptera: Ceratopogonidae) at the European level |
| title |
The tree that hides the forest: Cryptic diversity and phylogenetic relationships in the Palaearctic vector Obsoletus/Scoticus Complex (Diptera: Ceratopogonidae) at the European level |
| spellingShingle |
The tree that hides the forest: Cryptic diversity and phylogenetic relationships in the Palaearctic vector Obsoletus/Scoticus Complex (Diptera: Ceratopogonidae) at the European level Mignotte, A. |
| title_short |
The tree that hides the forest: Cryptic diversity and phylogenetic relationships in the Palaearctic vector Obsoletus/Scoticus Complex (Diptera: Ceratopogonidae) at the European level |
| title_full |
The tree that hides the forest: Cryptic diversity and phylogenetic relationships in the Palaearctic vector Obsoletus/Scoticus Complex (Diptera: Ceratopogonidae) at the European level |
| title_fullStr |
The tree that hides the forest: Cryptic diversity and phylogenetic relationships in the Palaearctic vector Obsoletus/Scoticus Complex (Diptera: Ceratopogonidae) at the European level |
| title_full_unstemmed |
The tree that hides the forest: Cryptic diversity and phylogenetic relationships in the Palaearctic vector Obsoletus/Scoticus Complex (Diptera: Ceratopogonidae) at the European level |
| title_sort |
The tree that hides the forest: Cryptic diversity and phylogenetic relationships in the Palaearctic vector Obsoletus/Scoticus Complex (Diptera: Ceratopogonidae) at the European level |
| dc.creator.none.fl_str_mv |
Mignotte, A. Garros, C. Gardès, L. Balenghien, T. Duhayon, M. Rakotoarivony, I. Tabourin, L. Poujol, L. Mathieu, B. Ibañez-Justicia, A. Deniz, A. Cvetkovikj, A. Purse, B.V. Ramilo, D.W. Stougiou, D. Werner, D. Pudar, D. Petric, D. Veronesi, E. Jacobs, F. Kampen, H. Pereira Da Fonseca, I. Lucientes, J. Navarro, J. De La Puente, J.M. Stefanovska, J. Searle, K.R. Khallaayoune, K. Culverwell, C.L. Larska, M. Bourquia, M. Goffredo, M. Bisia, M. England, M. Robin, M. Quaglia, M. Miranda-Chueca, M.Á. Bødker, R. Estrada-Penã, R. Carpenter, S. Tchakarova, S. Boutsini, S. Sviland, S. Schäfer, S.M. Ozolina¸ , Z. Seglina¸ , Z. Vatansever, Z. Huber, K. |
| author |
Mignotte, A. |
| author_facet |
Mignotte, A. Garros, C. Gardès, L. Balenghien, T. Duhayon, M. Rakotoarivony, I. Tabourin, L. Poujol, L. Mathieu, B. Ibañez-Justicia, A. Deniz, A. Cvetkovikj, A. Purse, B.V. Ramilo, D.W. Stougiou, D. Werner, D. Pudar, D. Petric, D. Veronesi, E. Jacobs, F. Kampen, H. Pereira Da Fonseca, I. Lucientes, J. Navarro, J. De La Puente, J.M. Stefanovska, J. Searle, K.R. Khallaayoune, K. Culverwell, C.L. Larska, M. Bourquia, M. Goffredo, M. Bisia, M. England, M. Robin, M. Quaglia, M. Miranda-Chueca, M.Á. Bødker, R. Estrada-Penã, R. Carpenter, S. Tchakarova, S. Boutsini, S. Sviland, S. Schäfer, S.M. Ozolina¸ , Z. Seglina¸ Vatansever, Z. Huber, K. |
| author_role |
author |
| author2 |
Garros, C. Gardès, L. Balenghien, T. Duhayon, M. Rakotoarivony, I. Tabourin, L. Poujol, L. Mathieu, B. Ibañez-Justicia, A. Deniz, A. Cvetkovikj, A. Purse, B.V. Ramilo, D.W. Stougiou, D. Werner, D. Pudar, D. Petric, D. Veronesi, E. Jacobs, F. Kampen, H. Pereira Da Fonseca, I. Lucientes, J. Navarro, J. De La Puente, J.M. Stefanovska, J. Searle, K.R. Khallaayoune, K. Culverwell, C.L. Larska, M. Bourquia, M. Goffredo, M. Bisia, M. England, M. Robin, M. Quaglia, M. Miranda-Chueca, M.Á. Bødker, R. Estrada-Penã, R. Carpenter, S. Tchakarova, S. Boutsini, S. Sviland, S. Schäfer, S.M. Ozolina¸ , Z. Seglina¸ Vatansever, Z. Huber, K. |
| author2_role |
author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author |
| description |
Background: Culicoides obsoletus is an abundant and widely distributed Holarctic biting midge species, involved in the transmission of bluetongue virus (BTV) and Schmallenberg virus (SBV) to wild and domestic ruminants. Females of this vector species are often reported jointly with two morphologically very close species, C. scoticus and C. montanus, forming the Obsoletus/Scoticus Complex. Recently, cryptic diversity within C. obsoletus was reported in geographically distant sites. Clear delineation of species and characterization of genetic variability is mandatory to revise their taxonomic status and assess the vector role of each taxonomic entity. Our objectives were to characterize and map the cryptic diversity within the Obsoletus/Scoticus Complex. Methods: Portion of the cox1 mitochondrial gene of 3763 individuals belonging to the Obsoletus/Scoticus Complex was sequenced. Populations from 20 countries along a Palaearctic Mediterranean transect covering Scandinavia to Canary islands (North to South) and Canary islands to Turkey (West to East) were included. Genetic diversity based on cox1 barcoding was supported by 16S rDNA mitochondrial gene sequences and a gene coding for ribosomal 28S rDNA. Species delimitation using a multi-marker methodology was used to revise the current taxonomic scheme of the Obsoletus/Scoticus Complex. Results: Our analysis showed the existence of three phylogenetic clades (C. obsoletus clade O2, C. obsoletus clade dark and one not yet named and identified) within C. obsoletus. These analyses also revealed two intra-specific clades within C. scoticus and raised questions about the taxonomic status of C. montanus. Conclusions: To our knowledge, our study provides the first genetic characterization of the Obsoletus/Scoticus Complex on a large geographical scale and allows a revision of the current taxonomic classification for an important group of vector species of livestock viruses in the Palaearctic region.[Figure not available: See fulltext.] |
| publishDate |
2020 |
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2020 |
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info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
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article |
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http://zaguan.unizar.es/record/89741 |
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Inglés |
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Inglés |
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info:eu-repo/grantAgreement/EC/ECDC/OC-EFSA-AHAW-2013-02-FWC1 VectorNet info:eu-repo/grantAgreement/EC/H2020/727393 This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 727393-PALE-Blu |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Universidad de Zaragoza |
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Zaguán. Repositorio Digital de la Universidad de Zaragoza |
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