Interaction between macrophages and nanoparticles

Understanding the modes of interaction between human monocytes/macrophages and engineered nanoparticles is the basis for assessing particle safety, in terms of activation of innate/inflammatory reactions, and their possible exploitation for medical applications. In vitro assessment of nanoparticle-m...

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Autores: Swartzwelter, Benjamin|||0000-0002-6283-5199, Verde, Alessandro, Rehak, Laura, Madej, Mariusz, Puntes, Víctor|||0000-0001-8996-9499, De Luca, Anna Chiara|||0000-0002-3696-8465, Boraschi, Diana|||0000-0002-3953-4056, Italiani, Paola|||0000-0001-9830-2733
Tipo de recurso: artículo
Fecha de publicación:2021
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:240989
Acceso en línea:https://ddd.uab.cat/record/240989
https://dx.doi.org/urn:doi:10.3390/nano11010207
Access Level:acceso abierto
Palabra clave:Monocytes
Macrophages
Gold nanoparticles
In vitro models
Innate immunity
Inflammation
Innate memory
2D cultures
3D cultures
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spelling Interaction between macrophages and nanoparticlesin vitro 3d cultures for the realistic assessment of inflammatory activation and modulation of innate memorySwartzwelter, Benjamin|||0000-0002-6283-5199Verde, AlessandroRehak, LauraMadej, MariuszPuntes, Víctor|||0000-0001-8996-9499De Luca, Anna Chiara|||0000-0002-3696-8465Boraschi, Diana|||0000-0002-3953-4056Italiani, Paola|||0000-0001-9830-2733MonocytesMacrophagesGold nanoparticlesIn vitro modelsInnate immunityInflammationInnate memory2D cultures3D culturesUnderstanding the modes of interaction between human monocytes/macrophages and engineered nanoparticles is the basis for assessing particle safety, in terms of activation of innate/inflammatory reactions, and their possible exploitation for medical applications. In vitro assessment of nanoparticle-macrophage interaction allows for examining the response of primary human cells, but the conventional 2D cultures do not reproduce the three-dimensional spacing of a tissue and the interaction of macrophages with the extracellular tissue matrix, conditions that shape macrophage recognition capacity and reactivity. Here, we have compared traditional 2D cultures with cultures on a 3D collagen matrix for evaluating the capacity gold nanoparticles to induce monocyte activation and subsequent innate memory in human blood monocytes in comparison to bacterial LPS. Results show that monocytes react to stimuli almost in the same way in 2D and 3D cultures in terms of production of TNFα and IL-6, but that notable differences are found when IL-8 and IL-1Ra are examined, in particular in the recall/memory response of primed cells to a second stimulation, with the 3D cultures showing cell activation and memory effects of nanoparticles better. In addition, the response variations in monocytes/macrophages from different donors point towards a personalized assessment of the nanoparticle effects on macrophage activation. 22021-01-0120212021-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/240989https://dx.doi.org/urn:doi:10.3390/nano11010207reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengEuropean Commission https://doi.org/10.13039/501100000780 316383European Commission https://doi.org/10.13039/501100000780 671881European Commission https://doi.org/10.13039/501100000780 812661open accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:2409892026-06-06T12:50:31Z
dc.title.none.fl_str_mv Interaction between macrophages and nanoparticles
in vitro 3d cultures for the realistic assessment of inflammatory activation and modulation of innate memory
title Interaction between macrophages and nanoparticles
spellingShingle Interaction between macrophages and nanoparticles
Swartzwelter, Benjamin|||0000-0002-6283-5199
Monocytes
Macrophages
Gold nanoparticles
In vitro models
Innate immunity
Inflammation
Innate memory
2D cultures
3D cultures
title_short Interaction between macrophages and nanoparticles
title_full Interaction between macrophages and nanoparticles
title_fullStr Interaction between macrophages and nanoparticles
title_full_unstemmed Interaction between macrophages and nanoparticles
title_sort Interaction between macrophages and nanoparticles
dc.creator.none.fl_str_mv Swartzwelter, Benjamin|||0000-0002-6283-5199
Verde, Alessandro
Rehak, Laura
Madej, Mariusz
Puntes, Víctor|||0000-0001-8996-9499
De Luca, Anna Chiara|||0000-0002-3696-8465
Boraschi, Diana|||0000-0002-3953-4056
Italiani, Paola|||0000-0001-9830-2733
author Swartzwelter, Benjamin|||0000-0002-6283-5199
author_facet Swartzwelter, Benjamin|||0000-0002-6283-5199
Verde, Alessandro
Rehak, Laura
Madej, Mariusz
Puntes, Víctor|||0000-0001-8996-9499
De Luca, Anna Chiara|||0000-0002-3696-8465
Boraschi, Diana|||0000-0002-3953-4056
Italiani, Paola|||0000-0001-9830-2733
author_role author
author2 Verde, Alessandro
Rehak, Laura
Madej, Mariusz
Puntes, Víctor|||0000-0001-8996-9499
De Luca, Anna Chiara|||0000-0002-3696-8465
Boraschi, Diana|||0000-0002-3953-4056
Italiani, Paola|||0000-0001-9830-2733
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Monocytes
Macrophages
Gold nanoparticles
In vitro models
Innate immunity
Inflammation
Innate memory
2D cultures
3D cultures
topic Monocytes
Macrophages
Gold nanoparticles
In vitro models
Innate immunity
Inflammation
Innate memory
2D cultures
3D cultures
description Understanding the modes of interaction between human monocytes/macrophages and engineered nanoparticles is the basis for assessing particle safety, in terms of activation of innate/inflammatory reactions, and their possible exploitation for medical applications. In vitro assessment of nanoparticle-macrophage interaction allows for examining the response of primary human cells, but the conventional 2D cultures do not reproduce the three-dimensional spacing of a tissue and the interaction of macrophages with the extracellular tissue matrix, conditions that shape macrophage recognition capacity and reactivity. Here, we have compared traditional 2D cultures with cultures on a 3D collagen matrix for evaluating the capacity gold nanoparticles to induce monocyte activation and subsequent innate memory in human blood monocytes in comparison to bacterial LPS. Results show that monocytes react to stimuli almost in the same way in 2D and 3D cultures in terms of production of TNFα and IL-6, but that notable differences are found when IL-8 and IL-1Ra are examined, in particular in the recall/memory response of primed cells to a second stimulation, with the 3D cultures showing cell activation and memory effects of nanoparticles better. In addition, the response variations in monocytes/macrophages from different donors point towards a personalized assessment of the nanoparticle effects on macrophage activation.
publishDate 2021
dc.date.none.fl_str_mv 2
2021-01-01
2021
2021-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/240989
https://dx.doi.org/urn:doi:10.3390/nano11010207
url https://ddd.uab.cat/record/240989
https://dx.doi.org/urn:doi:10.3390/nano11010207
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv European Commission https://doi.org/10.13039/501100000780 316383
European Commission https://doi.org/10.13039/501100000780 671881
European Commission https://doi.org/10.13039/501100000780 812661
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.300719

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