A Noninvasive Method for Time-Lapse Imaging of Microbial Interactions and Colony Dynamics.

Complex interactions between microbial populations can greatly affect the overall properties of a microbial community, sometimes leading to cooperation and mutually beneficial coexistence, or competition and the death or displacement of organisms or subpopulations. Interactions between different bio...

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Detalles Bibliográficos
Autores: Molina-Santiago, Carlos, Pearson, John R, Berlanga-Clavero, María Victoria, Pérez-Lorente, Alicia Isabel, de Vicente, Antonio, Romero, Diego
Tipo de recurso: artículo
Fecha de publicación:2022
País:España
Institución:Instituto de Salud Carlos III (ISCIII)
Repositorio:Repisalud
Idioma:inglés
OAI Identifier:oai:repisalud.isciii.es:20.500.12105/18700
Acceso en línea:http://hdl.handle.net/20.500.12105/18700
Access Level:acceso abierto
Palabra clave:biofilms
microbe-microbe interactions
microbial communities
Bacteria
Biofilms
Microbial Interactions
Microbiota
Time-Lapse Imaging
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spelling A Noninvasive Method for Time-Lapse Imaging of Microbial Interactions and Colony Dynamics.Molina-Santiago, CarlosPearson, John RBerlanga-Clavero, María VictoriaPérez-Lorente, Alicia Isabelde Vicente, AntonioRomero, Diegobiofilmsmicrobe-microbe interactionsmicrobial communitiesBacteriaBiofilmsMicrobial InteractionsMicrobiotaTime-Lapse ImagingComplex interactions between microbial populations can greatly affect the overall properties of a microbial community, sometimes leading to cooperation and mutually beneficial coexistence, or competition and the death or displacement of organisms or subpopulations. Interactions between different biofilm populations are highly relevant in diverse scientific areas, from antimicrobial resistance to microbial ecology. The utilization of modern microscopic techniques has provided a new and interesting insight into how bacteria interact at the cellular level to form and maintain microbial biofilms. However, our ability to follow complex intraspecies and interspecies interactions in vivo at the microscopic level has remained somewhat limited. Here, we detailed BacLive, a novel noninvasive method for tracking bacterial growth and biofilm dynamics using high-resolution fluorescence microscopy and an associated ImageJ processing macro (https://github.com/BacLive) for easier data handling and image analysis. Finally, we provided examples of how BacLive can be used in the analysis of complex bacterial communities. IMPORTANCE Communication and interactions between single cells are continuously defining the structure and composition of microbial communities temporally and spatially. Methods routinely used to study these communities at the cellular level rely on sample manipulation which makes microscopic time-lapse experiments impossible. BacLive was conceived as a method for the noninvasive study of the formation and development of bacterial communities, such as biofilms, and the formation dynamics of specialized subpopulations in time-lapse experiments at a colony level. In addition, we developed a tool to simplify the processing and analysis of the data generated by this method.20242024-02-2720222022-07-0520222022-07-05research articlehttp://purl.org/coar/resource_type/c_2df8fbb1VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articlehttp://hdl.handle.net/20.500.12105/18700reponame:Repisaludinstname:Instituto de Salud Carlos III (ISCIII)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:repisalud.isciii.es:20.500.12105/187002026-06-12T12:43:37Z
dc.title.none.fl_str_mv A Noninvasive Method for Time-Lapse Imaging of Microbial Interactions and Colony Dynamics.
title A Noninvasive Method for Time-Lapse Imaging of Microbial Interactions and Colony Dynamics.
spellingShingle A Noninvasive Method for Time-Lapse Imaging of Microbial Interactions and Colony Dynamics.
Molina-Santiago, Carlos
biofilms
microbe-microbe interactions
microbial communities
Bacteria
Biofilms
Microbial Interactions
Microbiota
Time-Lapse Imaging
title_short A Noninvasive Method for Time-Lapse Imaging of Microbial Interactions and Colony Dynamics.
title_full A Noninvasive Method for Time-Lapse Imaging of Microbial Interactions and Colony Dynamics.
title_fullStr A Noninvasive Method for Time-Lapse Imaging of Microbial Interactions and Colony Dynamics.
title_full_unstemmed A Noninvasive Method for Time-Lapse Imaging of Microbial Interactions and Colony Dynamics.
title_sort A Noninvasive Method for Time-Lapse Imaging of Microbial Interactions and Colony Dynamics.
dc.creator.none.fl_str_mv Molina-Santiago, Carlos
Pearson, John R
Berlanga-Clavero, María Victoria
Pérez-Lorente, Alicia Isabel
de Vicente, Antonio
Romero, Diego
author Molina-Santiago, Carlos
author_facet Molina-Santiago, Carlos
Pearson, John R
Berlanga-Clavero, María Victoria
Pérez-Lorente, Alicia Isabel
de Vicente, Antonio
Romero, Diego
author_role author
author2 Pearson, John R
Berlanga-Clavero, María Victoria
Pérez-Lorente, Alicia Isabel
de Vicente, Antonio
Romero, Diego
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv
dc.subject.none.fl_str_mv biofilms
microbe-microbe interactions
microbial communities
Bacteria
Biofilms
Microbial Interactions
Microbiota
Time-Lapse Imaging
topic biofilms
microbe-microbe interactions
microbial communities
Bacteria
Biofilms
Microbial Interactions
Microbiota
Time-Lapse Imaging
description Complex interactions between microbial populations can greatly affect the overall properties of a microbial community, sometimes leading to cooperation and mutually beneficial coexistence, or competition and the death or displacement of organisms or subpopulations. Interactions between different biofilm populations are highly relevant in diverse scientific areas, from antimicrobial resistance to microbial ecology. The utilization of modern microscopic techniques has provided a new and interesting insight into how bacteria interact at the cellular level to form and maintain microbial biofilms. However, our ability to follow complex intraspecies and interspecies interactions in vivo at the microscopic level has remained somewhat limited. Here, we detailed BacLive, a novel noninvasive method for tracking bacterial growth and biofilm dynamics using high-resolution fluorescence microscopy and an associated ImageJ processing macro (https://github.com/BacLive) for easier data handling and image analysis. Finally, we provided examples of how BacLive can be used in the analysis of complex bacterial communities. IMPORTANCE Communication and interactions between single cells are continuously defining the structure and composition of microbial communities temporally and spatially. Methods routinely used to study these communities at the cellular level rely on sample manipulation which makes microscopic time-lapse experiments impossible. BacLive was conceived as a method for the noninvasive study of the formation and development of bacterial communities, such as biofilms, and the formation dynamics of specialized subpopulations in time-lapse experiments at a colony level. In addition, we developed a tool to simplify the processing and analysis of the data generated by this method.
publishDate 2022
dc.date.none.fl_str_mv 2022
2022-07-05
2022
2022-07-05
2024
2024-02-27
dc.type.none.fl_str_mv research article
http://purl.org/coar/resource_type/c_2df8fbb1
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12105/18700
url http://hdl.handle.net/20.500.12105/18700
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repisalud
instname:Instituto de Salud Carlos III (ISCIII)
instname_str Instituto de Salud Carlos III (ISCIII)
reponame_str Repisalud
collection Repisalud
repository.name.fl_str_mv
repository.mail.fl_str_mv
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