Paternal Inheritance of Bisphenol A Cardiotoxic Effects: The Implications of Sperm Epigenome

[EN] Parental exposure to bisphenol A (BPA) has been linked to a greater incidence of congenital diseases. We have demonstrated that BPA induces in zebrafish males an increase in the acetylation of sperm histones that is transmitted to the blastomeres of the unexposed progeny. This work is aimed to...

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Detalles Bibliográficos
Autores: Lombó Alonso, Marta, Herráez Ortega, María Paz
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:España
Institución:Universidad Rey Juan Carlos
Repositorio:BULERIA. Repositorio Institucional de la Universidad de León
OAI Identifier:oai:buleria.unileon.es:10612/18095
Acceso en línea:https://www.mdpi.com/1422-0067/22/4/2125
https://hdl.handle.net/10612/18095
Access Level:acceso abierto
Palabra clave:Biología
Cardiogenesis
Zebrafish
Paternal transmission
Epigenetics
Bisphenol A
Paternal exposure
Histone acetylation
Sperm epigenome
Heart development
2401.07 Embriología Animal
2407 Biología Celular
3214 Toxicología
2409.99 Otros (Epigenética)
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spelling Paternal Inheritance of Bisphenol A Cardiotoxic Effects: The Implications of Sperm EpigenomeLombó Alonso, MartaHerráez Ortega, María PazBiologíaCardiogenesisZebrafishPaternal transmissionEpigeneticsBisphenol APaternal exposureHistone acetylationSperm epigenomeHeart development2401.07 Embriología Animal2407 Biología Celular3214 Toxicología2409.99 Otros (Epigenética)[EN] Parental exposure to bisphenol A (BPA) has been linked to a greater incidence of congenital diseases. We have demonstrated that BPA induces in zebrafish males an increase in the acetylation of sperm histones that is transmitted to the blastomeres of the unexposed progeny. This work is aimed to determine whether histone hyperacetylation promoted by paternal exposure to BPA is the molecular mechanism underlying the cardiogenesis impairment in the descendants. Zebrafish males were exposed to 100 and 2000 µg/L BPA during early spermatogenesis and mated with non-exposed females. We analyzed in the progeny the expression of genes involved in cardiogenesis and the epigenetic profile. Once the histone hyperacetylation was confirmed, treatment with epigallocatechin gallate (EGCG), an inhibitor of histone acetyltransferases, was assayed on F1 embryos. Embryos from males exposed to 2000 µg/L BPA overexpressed the transcription factor hand2 and the receptor esr2b, showing their own promoters—as well as that of kat6a—an enrichment in H3K9ac. In embryos treated with EGCG, both gene expression and histone acetylation (global and specific) returned to basal levels, and the phenotype was recovered. As shown by the results, the histone hyperacetylated landscape promoted by BPA in the sperm alters the chromatin structure of the progeny, leading to the overexpression of the histone acetyltransferase and genes involved in cardiogenesisSIThis work was funded by the Spanish Ministry of Economy and Competitiveness (Project AGL2014-53167-C3-3-R; PhD Grant BES- 2015-071885) and it was awarded by Fundación Carolina Rodríguez (Universidad de León) in the 24th edition of “Premios Mariano Rodríguez para jóvenes investigadores”MDPIBiologia CelularFacultad de Ciencias Biologicas y Ambientales2021info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://www.mdpi.com/1422-0067/22/4/2125https://hdl.handle.net/10612/18095reponame:BULERIA. Repositorio Institucional de la Universidad de Leóninstname:Universidad Rey Juan CarlosInglésinfo:eu-repo/grantAgreement/MINECO/Programa Estatal de I+D+I Orientada a los Retos de la Sociedad/AGL2014-53167-C3-3-Rinfo:eu-repo/grantAgreement/MINECO/Programa Estatal de Promoción del Talento y su Empleabilidad/BES- 2015-071885https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:buleria.unileon.es:10612/180952026-06-24T12:43:27Z
dc.title.none.fl_str_mv Paternal Inheritance of Bisphenol A Cardiotoxic Effects: The Implications of Sperm Epigenome
title Paternal Inheritance of Bisphenol A Cardiotoxic Effects: The Implications of Sperm Epigenome
spellingShingle Paternal Inheritance of Bisphenol A Cardiotoxic Effects: The Implications of Sperm Epigenome
Lombó Alonso, Marta
Biología
Cardiogenesis
Zebrafish
Paternal transmission
Epigenetics
Bisphenol A
Paternal exposure
Histone acetylation
Sperm epigenome
Heart development
2401.07 Embriología Animal
2407 Biología Celular
3214 Toxicología
2409.99 Otros (Epigenética)
title_short Paternal Inheritance of Bisphenol A Cardiotoxic Effects: The Implications of Sperm Epigenome
title_full Paternal Inheritance of Bisphenol A Cardiotoxic Effects: The Implications of Sperm Epigenome
title_fullStr Paternal Inheritance of Bisphenol A Cardiotoxic Effects: The Implications of Sperm Epigenome
title_full_unstemmed Paternal Inheritance of Bisphenol A Cardiotoxic Effects: The Implications of Sperm Epigenome
title_sort Paternal Inheritance of Bisphenol A Cardiotoxic Effects: The Implications of Sperm Epigenome
dc.creator.none.fl_str_mv Lombó Alonso, Marta
Herráez Ortega, María Paz
author Lombó Alonso, Marta
author_facet Lombó Alonso, Marta
Herráez Ortega, María Paz
author_role author
author2 Herráez Ortega, María Paz
author2_role author
dc.contributor.none.fl_str_mv Biologia Celular
Facultad de Ciencias Biologicas y Ambientales
dc.subject.none.fl_str_mv Biología
Cardiogenesis
Zebrafish
Paternal transmission
Epigenetics
Bisphenol A
Paternal exposure
Histone acetylation
Sperm epigenome
Heart development
2401.07 Embriología Animal
2407 Biología Celular
3214 Toxicología
2409.99 Otros (Epigenética)
topic Biología
Cardiogenesis
Zebrafish
Paternal transmission
Epigenetics
Bisphenol A
Paternal exposure
Histone acetylation
Sperm epigenome
Heart development
2401.07 Embriología Animal
2407 Biología Celular
3214 Toxicología
2409.99 Otros (Epigenética)
description [EN] Parental exposure to bisphenol A (BPA) has been linked to a greater incidence of congenital diseases. We have demonstrated that BPA induces in zebrafish males an increase in the acetylation of sperm histones that is transmitted to the blastomeres of the unexposed progeny. This work is aimed to determine whether histone hyperacetylation promoted by paternal exposure to BPA is the molecular mechanism underlying the cardiogenesis impairment in the descendants. Zebrafish males were exposed to 100 and 2000 µg/L BPA during early spermatogenesis and mated with non-exposed females. We analyzed in the progeny the expression of genes involved in cardiogenesis and the epigenetic profile. Once the histone hyperacetylation was confirmed, treatment with epigallocatechin gallate (EGCG), an inhibitor of histone acetyltransferases, was assayed on F1 embryos. Embryos from males exposed to 2000 µg/L BPA overexpressed the transcription factor hand2 and the receptor esr2b, showing their own promoters—as well as that of kat6a—an enrichment in H3K9ac. In embryos treated with EGCG, both gene expression and histone acetylation (global and specific) returned to basal levels, and the phenotype was recovered. As shown by the results, the histone hyperacetylated landscape promoted by BPA in the sperm alters the chromatin structure of the progeny, leading to the overexpression of the histone acetyltransferase and genes involved in cardiogenesis
publishDate 2021
dc.date.none.fl_str_mv 2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://www.mdpi.com/1422-0067/22/4/2125
https://hdl.handle.net/10612/18095
url https://www.mdpi.com/1422-0067/22/4/2125
https://hdl.handle.net/10612/18095
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/MINECO/Programa Estatal de I+D+I Orientada a los Retos de la Sociedad/AGL2014-53167-C3-3-R
info:eu-repo/grantAgreement/MINECO/Programa Estatal de Promoción del Talento y su Empleabilidad/BES- 2015-071885
dc.rights.none.fl_str_mv https://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:BULERIA. Repositorio Institucional de la Universidad de León
instname:Universidad Rey Juan Carlos
instname_str Universidad Rey Juan Carlos
reponame_str BULERIA. Repositorio Institucional de la Universidad de León
collection BULERIA. Repositorio Institucional de la Universidad de León
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300719

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