Molecular Epidemiology of Pasteurella multocida Associated with Bovine Respiratory Disease Outbreaks

Studies that characterize bovine respiratory disease (BRD)-associated Pasteurella multocida isolates are scarce compared with research on isolates from other hosts and clinical backgrounds. In the present study, 170 P. multocida isolates from 125 BRD outbreaks were characterized by capsular and LPS...

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Autores: Calderón Bernal, Johan Manuel, Fernández, Ana, Arnal, José Luis, Sanz Tejero, Celia, Vela Alonso, Ana Isabel, Fernández-Garayzábal Fernández, José Francisco, Cid Vázquez, María Dolores
Tipo de recurso: artículo
Fecha de publicación:2022
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/73198
Acceso en línea:https://hdl.handle.net/20.500.14352/73198
Access Level:acceso abierto
Palabra clave:Bovine respiratory disease
Pasteurella multocida
Outbreaks
MLST
PFGE
Virulotyping
Cattle
Ganado vacuno
Inmunología veterinaria
3104.07 Ovinos
3109.03 Inmunología
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network_name_str España
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spelling Molecular Epidemiology of Pasteurella multocida Associated with Bovine Respiratory Disease OutbreaksCalderón Bernal, Johan ManuelFernández, AnaArnal, José LuisSanz Tejero, CeliaVela Alonso, Ana IsabelFernández-Garayzábal Fernández, José FranciscoCid Vázquez, María DoloresBovine respiratory diseasePasteurella multocidaOutbreaksMLSTPFGEVirulotypingCattleGanado vacunoInmunología veterinaria3104.07 Ovinos3109.03 InmunologíaStudies that characterize bovine respiratory disease (BRD)-associated Pasteurella multocida isolates are scarce compared with research on isolates from other hosts and clinical backgrounds. In the present study, 170 P. multocida isolates from 125 BRD outbreaks were characterized by capsular and LPS typing as well as by virulotyping. Three capsular types (A, B, F) and three LPS genotypes (L2, L3, L6) were identified. Capsular and LPS typing revealed a very low genetic diversity (GD = 0.02) among P. multocida, with most isolates belonging to genotype A:L3 (97.6%). Virulotyping identified seven virulence-associated gene profiles, with two profiles including 95.9% of the isolates. A subset of isolates was further characterized by MLST and PFGE. The sequence types ST79 and ST13 were the most frequently identified and were grouped into the same clonal complex (CC13), a result that supports the clonal population structure of BRD-associated P. multocida isolates. PFGE typing also revealed a low genetic diversity (GD = 0.18), detecting a single pattern in 62.5% of the outbreaks in which multiple isolates were analyzed. Overall, 85.2% of the isolates belonged to pulsotypes with at least 80% genetic similarity, consistent with a clonal population structure observed by MLST analysis and corroborating the genetic relatedness of most P. multocida isolates associated with BRD in cattle.MPDIUniversidad Complutense de Madrid20222022-12-2420222022-12-24journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/73198reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Atribución 3.0 Españahttps://creativecommons.org/licenses/by/3.0/es/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/731982026-06-02T12:44:21Z
dc.title.none.fl_str_mv Molecular Epidemiology of Pasteurella multocida Associated with Bovine Respiratory Disease Outbreaks
title Molecular Epidemiology of Pasteurella multocida Associated with Bovine Respiratory Disease Outbreaks
spellingShingle Molecular Epidemiology of Pasteurella multocida Associated with Bovine Respiratory Disease Outbreaks
Calderón Bernal, Johan Manuel
Bovine respiratory disease
Pasteurella multocida
Outbreaks
MLST
PFGE
Virulotyping
Cattle
Ganado vacuno
Inmunología veterinaria
3104.07 Ovinos
3109.03 Inmunología
title_short Molecular Epidemiology of Pasteurella multocida Associated with Bovine Respiratory Disease Outbreaks
title_full Molecular Epidemiology of Pasteurella multocida Associated with Bovine Respiratory Disease Outbreaks
title_fullStr Molecular Epidemiology of Pasteurella multocida Associated with Bovine Respiratory Disease Outbreaks
title_full_unstemmed Molecular Epidemiology of Pasteurella multocida Associated with Bovine Respiratory Disease Outbreaks
title_sort Molecular Epidemiology of Pasteurella multocida Associated with Bovine Respiratory Disease Outbreaks
dc.creator.none.fl_str_mv Calderón Bernal, Johan Manuel
Fernández, Ana
Arnal, José Luis
Sanz Tejero, Celia
Vela Alonso, Ana Isabel
Fernández-Garayzábal Fernández, José Francisco
Cid Vázquez, María Dolores
author Calderón Bernal, Johan Manuel
author_facet Calderón Bernal, Johan Manuel
Fernández, Ana
Arnal, José Luis
Sanz Tejero, Celia
Vela Alonso, Ana Isabel
Fernández-Garayzábal Fernández, José Francisco
Cid Vázquez, María Dolores
author_role author
author2 Fernández, Ana
Arnal, José Luis
Sanz Tejero, Celia
Vela Alonso, Ana Isabel
Fernández-Garayzábal Fernández, José Francisco
Cid Vázquez, María Dolores
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv Bovine respiratory disease
Pasteurella multocida
Outbreaks
MLST
PFGE
Virulotyping
Cattle
Ganado vacuno
Inmunología veterinaria
3104.07 Ovinos
3109.03 Inmunología
topic Bovine respiratory disease
Pasteurella multocida
Outbreaks
MLST
PFGE
Virulotyping
Cattle
Ganado vacuno
Inmunología veterinaria
3104.07 Ovinos
3109.03 Inmunología
description Studies that characterize bovine respiratory disease (BRD)-associated Pasteurella multocida isolates are scarce compared with research on isolates from other hosts and clinical backgrounds. In the present study, 170 P. multocida isolates from 125 BRD outbreaks were characterized by capsular and LPS typing as well as by virulotyping. Three capsular types (A, B, F) and three LPS genotypes (L2, L3, L6) were identified. Capsular and LPS typing revealed a very low genetic diversity (GD = 0.02) among P. multocida, with most isolates belonging to genotype A:L3 (97.6%). Virulotyping identified seven virulence-associated gene profiles, with two profiles including 95.9% of the isolates. A subset of isolates was further characterized by MLST and PFGE. The sequence types ST79 and ST13 were the most frequently identified and were grouped into the same clonal complex (CC13), a result that supports the clonal population structure of BRD-associated P. multocida isolates. PFGE typing also revealed a low genetic diversity (GD = 0.18), detecting a single pattern in 62.5% of the outbreaks in which multiple isolates were analyzed. Overall, 85.2% of the isolates belonged to pulsotypes with at least 80% genetic similarity, consistent with a clonal population structure observed by MLST analysis and corroborating the genetic relatedness of most P. multocida isolates associated with BRD in cattle.
publishDate 2022
dc.date.none.fl_str_mv 2022
2022-12-24
2022
2022-12-24
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/73198
url https://hdl.handle.net/20.500.14352/73198
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 3.0 España
https://creativecommons.org/licenses/by/3.0/es/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 3.0 España
https://creativecommons.org/licenses/by/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MPDI
publisher.none.fl_str_mv MPDI
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300719

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