Shotgun proteomic analysis to study the decrease of xenograft tumor growth after rosemary extract treatment

The antiproliferative activity of Rosemary (Rosmarinus officinalis) has been widely studied in different in vitro and in vivo models, which demonstrate that rosemary extracts inhibit the cellular proliferation due to its ability to interact with a wide spectrum of molecular targets. However, a compr...

Descripción completa

Detalles Bibliográficos
Autores: Valdés, Alberto, García-Cañas, Virginia, Pérez-Sánchez, Almudena, Barrajón-Catalán, Enrique, Ruiz-Torres, Verónica, Artemenko, Konstantin A., Micol, Vicente, Bergquist, Jonas, Cifuentes, Alejandro
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2017
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/194213
Acceso en línea:http://hdl.handle.net/10261/194213
Access Level:acceso abierto
Palabra clave:Colon cancer
Dimethyl labeling
Mass spectrometry
Rosemary extract
Quantitative proteomics
Xenograft mice
id ES_c6e80f6fb63fee8a11f87aa128db25e2
oai_identifier_str oai:digital.csic.es:10261/194213
network_acronym_str ES
network_name_str España
repository_id_str
spelling Shotgun proteomic analysis to study the decrease of xenograft tumor growth after rosemary extract treatmentValdés, AlbertoGarcía-Cañas, VirginiaPérez-Sánchez, AlmudenaBarrajón-Catalán, EnriqueRuiz-Torres, VerónicaArtemenko, Konstantin A.Micol, VicenteBergquist, JonasCifuentes, AlejandroColon cancerDimethyl labelingMass spectrometryRosemary extractQuantitative proteomicsXenograft miceThe antiproliferative activity of Rosemary (Rosmarinus officinalis) has been widely studied in different in vitro and in vivo models, which demonstrate that rosemary extracts inhibit the cellular proliferation due to its ability to interact with a wide spectrum of molecular targets. However, a comprehensive proteomics study in vivo has not been carried out yet. In the present work, the effects of rosemary extract on xenograft tumor growth has been studied and, for the first time, a shotgun proteomic analysis based on nano-LC-MS/MS together with stable isotope dimethyl labeling (DML) has been applied to investigate the global protein changes in vivo. Our results show that the daily administration of a polyphenol-enriched rosemary extract reduces the progression of colorectal cancer in vivo with the subsequent deregulation of 74 proteins. The bioinformatic analysis of these proteins indicates that the rosemary extract mainly alters the RNA Post-Transcriptional Modification, the Protein Synthesis and the Amino Acid Metabolism functions and suggests the inactivation of the oncogene MYC. These results demonstrate the high utility of the proposed analytical methodology to determine, simultaneously, the expression levels of a large number of protein biomarkers and to generate new hypothesis about the molecular mechanisms of this extract in vivo.This work was supported by projects AGL2014-53609-P and AGL2015-67995-C3-1-R (Ministerio de Economía y Competitividad, Spain), S2013/ABI-2728 (Comunidad de Madrid), PROMETEO/2016/006 (Generalitat Valenciana) and CIBER (CB12/03/30038, Fisiopatologia de la Obesidad y la Nutrición, CIBERobn, Instituto de Salud Carlos III). A.V. thanks the Ministerio de Economía y Competitividad for his FPI pre-doctoral fellowship (BES-2012-057014). The Swedish Research Council (Vetenskapsrådet), 2011-4423 and 2015-4870 to J.B. is acknowledged for financial support.Peer reviewedElsevierMinisterio de Economía y Competitividad (España)Comunidad de MadridGeneralitat ValencianaInstituto de Salud Carlos IIISwedish Research CouncilConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201920192017info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionapplication/pdfhttp://hdl.handle.net/10261/194213reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO//AGL2014-53609-Pinfo:eu-repo/grantAgreement/MINECO//AGL2015-67995-C3-1-RS2013/ABI-2728/ALIBIRDhttps://doi.org/10.1016/j.chroma.2017.03.072Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1942132026-05-22T06:33:51Z
dc.title.none.fl_str_mv Shotgun proteomic analysis to study the decrease of xenograft tumor growth after rosemary extract treatment
title Shotgun proteomic analysis to study the decrease of xenograft tumor growth after rosemary extract treatment
spellingShingle Shotgun proteomic analysis to study the decrease of xenograft tumor growth after rosemary extract treatment
Valdés, Alberto
Colon cancer
Dimethyl labeling
Mass spectrometry
Rosemary extract
Quantitative proteomics
Xenograft mice
title_short Shotgun proteomic analysis to study the decrease of xenograft tumor growth after rosemary extract treatment
title_full Shotgun proteomic analysis to study the decrease of xenograft tumor growth after rosemary extract treatment
title_fullStr Shotgun proteomic analysis to study the decrease of xenograft tumor growth after rosemary extract treatment
title_full_unstemmed Shotgun proteomic analysis to study the decrease of xenograft tumor growth after rosemary extract treatment
title_sort Shotgun proteomic analysis to study the decrease of xenograft tumor growth after rosemary extract treatment
dc.creator.none.fl_str_mv Valdés, Alberto
García-Cañas, Virginia
Pérez-Sánchez, Almudena
Barrajón-Catalán, Enrique
Ruiz-Torres, Verónica
Artemenko, Konstantin A.
Micol, Vicente
Bergquist, Jonas
Cifuentes, Alejandro
author Valdés, Alberto
author_facet Valdés, Alberto
García-Cañas, Virginia
Pérez-Sánchez, Almudena
Barrajón-Catalán, Enrique
Ruiz-Torres, Verónica
Artemenko, Konstantin A.
Micol, Vicente
Bergquist, Jonas
Cifuentes, Alejandro
author_role author
author2 García-Cañas, Virginia
Pérez-Sánchez, Almudena
Barrajón-Catalán, Enrique
Ruiz-Torres, Verónica
Artemenko, Konstantin A.
Micol, Vicente
Bergquist, Jonas
Cifuentes, Alejandro
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
Comunidad de Madrid
Generalitat Valenciana
Instituto de Salud Carlos III
Swedish Research Council
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Colon cancer
Dimethyl labeling
Mass spectrometry
Rosemary extract
Quantitative proteomics
Xenograft mice
topic Colon cancer
Dimethyl labeling
Mass spectrometry
Rosemary extract
Quantitative proteomics
Xenograft mice
description The antiproliferative activity of Rosemary (Rosmarinus officinalis) has been widely studied in different in vitro and in vivo models, which demonstrate that rosemary extracts inhibit the cellular proliferation due to its ability to interact with a wide spectrum of molecular targets. However, a comprehensive proteomics study in vivo has not been carried out yet. In the present work, the effects of rosemary extract on xenograft tumor growth has been studied and, for the first time, a shotgun proteomic analysis based on nano-LC-MS/MS together with stable isotope dimethyl labeling (DML) has been applied to investigate the global protein changes in vivo. Our results show that the daily administration of a polyphenol-enriched rosemary extract reduces the progression of colorectal cancer in vivo with the subsequent deregulation of 74 proteins. The bioinformatic analysis of these proteins indicates that the rosemary extract mainly alters the RNA Post-Transcriptional Modification, the Protein Synthesis and the Amino Acid Metabolism functions and suggests the inactivation of the oncogene MYC. These results demonstrate the high utility of the proposed analytical methodology to determine, simultaneously, the expression levels of a large number of protein biomarkers and to generate new hypothesis about the molecular mechanisms of this extract in vivo.
publishDate 2017
dc.date.none.fl_str_mv 2017
2019
2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/194213
url http://hdl.handle.net/10261/194213
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO//AGL2014-53609-P
info:eu-repo/grantAgreement/MINECO//AGL2015-67995-C3-1-R
S2013/ABI-2728/ALIBIRD
https://doi.org/10.1016/j.chroma.2017.03.072
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869419112035254272
score 15,811543

Similares en LA Referencia