Genome-wide gene expression profile induced by exposure to cadmium acetate in Leishmania infantum promastigotes.

11p.-5 fig.-3 tab.

Detalles Bibliográficos
Autores: Alcolea, Pedro J., Alonso, Ana, Larraga, Vicente
Tipo de recurso: artículo
Fecha de publicación:2011
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/101584
Acceso en línea:http://hdl.handle.net/10261/101584
Access Level:acceso abierto
Palabra clave:Leishmania infantum
L. infantum promastigotes
Cadmium
Translation factors
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spelling Genome-wide gene expression profile induced by exposure to cadmium acetate in Leishmania infantum promastigotes.Alcolea, Pedro J.Alonso, AnaLarraga, VicenteLeishmania infantumL. infantum promastigotesCadmiumTranslation factors11p.-5 fig.-3 tab.Leishmania infantum is the etiological agent of visceral leishmaniasis in Mediterranean areas. The life cycle of the protist is dimorphic and heteroxene, as promastigotes develop inside the gut of sand-fly vectors and amastigotes multiply inside mammalian phagocytic cells. In previous studies, we analyzed the expression profiles of these stages and the modulation of gene expression triggered by temperature increase and acidification, both of which are crucial in the differentiation of promastigotes to amastigotes. Differential expression profiles of translation initiation and elongation factors were detected. Here we report that the presence of 1 mM cadmium acetate in the culture medium leads to a shock response consisting of growth arrest, morphological changes, the absence of motility, and the up-regulation of genes that code for: a heavy metal transporter, trypanothione reductase, a haloacid-dehalogenase-like hydrolase, and a metalloexopeptidase from the M20 family, among others. This response is probably controlled by the differential expression of regulatory genes such as those encoding initiation factors 4E, eukaryotic translation initiation factor 3 subunits 8 and 2α, and elongation factor 1β. The initiation factor 2α gene is induced in anomalous environments, i.e., those outside of the protist’s normal life-cycle progression, for example, in response to the presence of cadmium ions, acidification without temperature increase, and vice versa. Our results suggest that the regulation of gene expression is a key component of the shock response.This study has been funded by the Ministry of Science and Innovation (MICINN) project AGL2007–66308-C02–01 project and RICET (RETICS) network fund (FEDER-MICINN). PJA thanks CSIC for I3P-BPD2003–1 fellowship and AA thanks CSIC for JAE-Doc027 (2008) contract.Peer reviewedConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201420142011info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://hdl.handle.net/10261/101584reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.2436/20.1501.01.129Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1015842026-05-22T06:33:51Z
dc.title.none.fl_str_mv Genome-wide gene expression profile induced by exposure to cadmium acetate in Leishmania infantum promastigotes.
title Genome-wide gene expression profile induced by exposure to cadmium acetate in Leishmania infantum promastigotes.
spellingShingle Genome-wide gene expression profile induced by exposure to cadmium acetate in Leishmania infantum promastigotes.
Alcolea, Pedro J.
Leishmania infantum
L. infantum promastigotes
Cadmium
Translation factors
title_short Genome-wide gene expression profile induced by exposure to cadmium acetate in Leishmania infantum promastigotes.
title_full Genome-wide gene expression profile induced by exposure to cadmium acetate in Leishmania infantum promastigotes.
title_fullStr Genome-wide gene expression profile induced by exposure to cadmium acetate in Leishmania infantum promastigotes.
title_full_unstemmed Genome-wide gene expression profile induced by exposure to cadmium acetate in Leishmania infantum promastigotes.
title_sort Genome-wide gene expression profile induced by exposure to cadmium acetate in Leishmania infantum promastigotes.
dc.creator.none.fl_str_mv Alcolea, Pedro J.
Alonso, Ana
Larraga, Vicente
author Alcolea, Pedro J.
author_facet Alcolea, Pedro J.
Alonso, Ana
Larraga, Vicente
author_role author
author2 Alonso, Ana
Larraga, Vicente
author2_role author
author
dc.contributor.none.fl_str_mv Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Leishmania infantum
L. infantum promastigotes
Cadmium
Translation factors
topic Leishmania infantum
L. infantum promastigotes
Cadmium
Translation factors
description 11p.-5 fig.-3 tab.
publishDate 2011
dc.date.none.fl_str_mv 2011
2014
2014
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/101584
url http://hdl.handle.net/10261/101584
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.2436/20.1501.01.129
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.812429

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