Exposure of pineapple shoot tips to liquid nitrogen and cryostorage do not affect the histological status of regenerated plantlets

[EN] Conservation of pineapple (Ananas comosus L. Merr.) genetic resources - including cryopreservation in liquid N-2 at -196 degrees C - is essential for future breeding programmes to develop new varieties with improved agronomic performance. However, the potentially deleterious effects of cryopres...

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Detalles Bibliográficos
Autores: Villalobos-Olivera, Ariel, Nápoles, Lelurlis, Mendoza, José Ramón, Escalante, Doris, Martínez, Julia, Concepción, Oscar, Zevallos, Byron E., Martínez-Montero, Marcos Edel, Cejas, Inaudis, Engelmann, Florent, Lorenzo, José Carlos, Vicente, Oscar|||0000-0001-5076-3784
Tipo de recurso: artículo
Fecha de publicación:2019
País:España
Institución:Universitat Politècnica de València (UPV)
Repositorio:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
Idioma:inglés
OAI Identifier:oai:riunet.upv.es:10251/160678
Acceso en línea:https://riunet.upv.es/handle/10251/160678
Access Level:acceso abierto
Palabra clave:Ananas comosus L. Merr.
Cryopreservation
Ex situ conservation
Genetic resources
Histology
BIOQUIMICA Y BIOLOGIA MOLECULAR
Descripción
Sumario:[EN] Conservation of pineapple (Ananas comosus L. Merr.) genetic resources - including cryopreservation in liquid N-2 at -196 degrees C - is essential for future breeding programmes to develop new varieties with improved agronomic performance. However, the potentially deleterious effects of cryopreservation on subsequent plant regrowth should be evaluated before large-scale development of cryobanks is implemented. This paper describes the histological analysis of pineapple plantlets regenerated from cryopreserved shoot tips. Two controls were included in the study: i) conventional micropropagation-derived plantlets, and ii) plants from shoot tips subjected to pre-cryostorage conditioning treatments but never exposed to liquid N-2. Histological studies of roots, leaves and stems were conducted after 45 days of hardening. No statistically significant differences with the controls were observed in any of the histological parameters evaluated, which supports the practical value of cryopreservation of pineapple germplasm.

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