Identification of Immunogenic Cytotoxic T Lymphocyte Epitopes Containing Drug Resistance Mutations in Antiretroviral Treatment-Naïve HIV-Infected Individuals

Background: therapeutic HIV vaccines may prove helpful to intensify antiretroviral treatment (ART) efficacy and may be an integral part of future cure strategies. Methods: we examined IFN-gamma ELISpot responses to a panel of 218 HIV clade B consensus-based HIV protease-reverse transcriptase peptide...

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Authors: Blanco-Heredia, Juan|||0000-0001-9526-7450, Lecanda, Aarón, Valenzuela Ponce, Humberto, Brander, Christian|||0000-0002-0548-5778, Avila-Rios, Santiago, Reyes Terán, Gustavo
Format: article
Publication Date:2016
Country:España
Institution:Universitat Autònoma de Barcelona
Repository:Dipòsit Digital de Documents de la UAB
Language:English
OAI Identifier:oai:ddd.uab.cat:174675
Online Access:https://ddd.uab.cat/record/174675
https://dx.doi.org/urn:doi:10.1371/journal.pone.0147571
Access Level:Open access
Keyword:SIDA
VIH (Virus)
Vacunes
Antiretroviral treatment-naïve
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oai_identifier_str oai:ddd.uab.cat:174675
network_acronym_str ES
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repository_id_str
spelling Identification of Immunogenic Cytotoxic T Lymphocyte Epitopes Containing Drug Resistance Mutations in Antiretroviral Treatment-Naïve HIV-Infected IndividualsBlanco-Heredia, Juan|||0000-0001-9526-7450Lecanda, AarónValenzuela Ponce, HumbertoBrander, Christian|||0000-0002-0548-5778Avila-Rios, SantiagoReyes Terán, GustavoSIDAVIH (Virus)VacunesAntiretroviral treatment-naïveBackground: therapeutic HIV vaccines may prove helpful to intensify antiretroviral treatment (ART) efficacy and may be an integral part of future cure strategies. Methods: we examined IFN-gamma ELISpot responses to a panel of 218 HIV clade B consensus-based HIV protease-reverse transcriptase peptides, designed to mimic previously described and predicted cytotoxic T lymphocyte epitopes overlapping drug resistance (DR) positions, that either included the consensus sequence or the DR variant sequence, in 49 ART-naïve HIV-infected individuals. Next generation sequencing was used to assess the presence of minority DR variants in circulating viral populations. Results: although a wide spectrum of differential magnitudes of response to DR vs. WT peptide pairs was observed, responses to DR peptides were frequent and strong in the study cohort. No difference between the median magnitudes of response to DR vs. WT peptides was observed. Interestingly, of the 22 peptides that were recognized by. 22016-01-0120162016-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/174675https://dx.doi.org/urn:doi:10.1371/journal.pone.0147571reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengopen accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:1746752026-06-06T12:50:31Z
dc.title.none.fl_str_mv Identification of Immunogenic Cytotoxic T Lymphocyte Epitopes Containing Drug Resistance Mutations in Antiretroviral Treatment-Naïve HIV-Infected Individuals
title Identification of Immunogenic Cytotoxic T Lymphocyte Epitopes Containing Drug Resistance Mutations in Antiretroviral Treatment-Naïve HIV-Infected Individuals
spellingShingle Identification of Immunogenic Cytotoxic T Lymphocyte Epitopes Containing Drug Resistance Mutations in Antiretroviral Treatment-Naïve HIV-Infected Individuals
Blanco-Heredia, Juan|||0000-0001-9526-7450
SIDA
VIH (Virus)
Vacunes
Antiretroviral treatment-naïve
title_short Identification of Immunogenic Cytotoxic T Lymphocyte Epitopes Containing Drug Resistance Mutations in Antiretroviral Treatment-Naïve HIV-Infected Individuals
title_full Identification of Immunogenic Cytotoxic T Lymphocyte Epitopes Containing Drug Resistance Mutations in Antiretroviral Treatment-Naïve HIV-Infected Individuals
title_fullStr Identification of Immunogenic Cytotoxic T Lymphocyte Epitopes Containing Drug Resistance Mutations in Antiretroviral Treatment-Naïve HIV-Infected Individuals
title_full_unstemmed Identification of Immunogenic Cytotoxic T Lymphocyte Epitopes Containing Drug Resistance Mutations in Antiretroviral Treatment-Naïve HIV-Infected Individuals
title_sort Identification of Immunogenic Cytotoxic T Lymphocyte Epitopes Containing Drug Resistance Mutations in Antiretroviral Treatment-Naïve HIV-Infected Individuals
dc.creator.none.fl_str_mv Blanco-Heredia, Juan|||0000-0001-9526-7450
Lecanda, Aarón
Valenzuela Ponce, Humberto
Brander, Christian|||0000-0002-0548-5778
Avila-Rios, Santiago
Reyes Terán, Gustavo
author Blanco-Heredia, Juan|||0000-0001-9526-7450
author_facet Blanco-Heredia, Juan|||0000-0001-9526-7450
Lecanda, Aarón
Valenzuela Ponce, Humberto
Brander, Christian|||0000-0002-0548-5778
Avila-Rios, Santiago
Reyes Terán, Gustavo
author_role author
author2 Lecanda, Aarón
Valenzuela Ponce, Humberto
Brander, Christian|||0000-0002-0548-5778
Avila-Rios, Santiago
Reyes Terán, Gustavo
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv SIDA
VIH (Virus)
Vacunes
Antiretroviral treatment-naïve
topic SIDA
VIH (Virus)
Vacunes
Antiretroviral treatment-naïve
description Background: therapeutic HIV vaccines may prove helpful to intensify antiretroviral treatment (ART) efficacy and may be an integral part of future cure strategies. Methods: we examined IFN-gamma ELISpot responses to a panel of 218 HIV clade B consensus-based HIV protease-reverse transcriptase peptides, designed to mimic previously described and predicted cytotoxic T lymphocyte epitopes overlapping drug resistance (DR) positions, that either included the consensus sequence or the DR variant sequence, in 49 ART-naïve HIV-infected individuals. Next generation sequencing was used to assess the presence of minority DR variants in circulating viral populations. Results: although a wide spectrum of differential magnitudes of response to DR vs. WT peptide pairs was observed, responses to DR peptides were frequent and strong in the study cohort. No difference between the median magnitudes of response to DR vs. WT peptides was observed. Interestingly, of the 22 peptides that were recognized by.
publishDate 2016
dc.date.none.fl_str_mv 2
2016-01-01
2016
2016-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/174675
https://dx.doi.org/urn:doi:10.1371/journal.pone.0147571
url https://ddd.uab.cat/record/174675
https://dx.doi.org/urn:doi:10.1371/journal.pone.0147571
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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