Protein aggregation and soluble aggregate formation screened by a fast microdialysis assay

Protein aggregation is a major obstacle in recombinant protein production as it reduces the yield of soluble polypeptides. Also, the formation of aggregates occurring in the soluble fraction is more common than formerly expected, and the prevalence of these entities might significantly affect the av...

Descripción completa

Detalles Bibliográficos
Autores: Toledo-Rubio, Verónica, Platas, Gemma, Domingo-Espin, Joan|||0000-0001-8149-0803, Unzueta Elorza, Ugutz|||0000-0001-5119-2266, Steinkamp, Elke, Garcia-Fruitos, Elena|||0000-0001-7498-4864, Ferrer-Miralles, Neus|||0000-0003-2981-3913, Villaverde, Antonio|||0000-0002-2615-4521
Tipo de recurso: artículo
Fecha de publicación:2010
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:137805
Acceso en línea:https://ddd.uab.cat/record/137805
https://dx.doi.org/urn:doi:10.1177/1087057110363911
Access Level:acceso abierto
Palabra clave:Protein aggregation
Nanoparticles
Dynamic light scattering
Proto-aggregates
Soluble aggregates
id ES_c6783efee3e89c7a42dabfc3f956e13b
oai_identifier_str oai:ddd.uab.cat:137805
network_acronym_str ES
network_name_str España
repository_id_str
spelling Protein aggregation and soluble aggregate formation screened by a fast microdialysis assayToledo-Rubio, VerónicaPlatas, GemmaDomingo-Espin, Joan|||0000-0001-8149-0803Unzueta Elorza, Ugutz|||0000-0001-5119-2266Steinkamp, ElkeGarcia-Fruitos, Elena|||0000-0001-7498-4864Ferrer-Miralles, Neus|||0000-0003-2981-3913Villaverde, Antonio|||0000-0002-2615-4521Protein aggregationNanoparticlesDynamic light scatteringProto-aggregatesSoluble aggregatesProtein aggregation is a major obstacle in recombinant protein production as it reduces the yield of soluble polypeptides. Also, the formation of aggregates occurring in the soluble fraction is more common than formerly expected, and the prevalence of these entities might significantly affect the average quality of the soluble protein species. Usually, the formation of soluble aggregates remains unperceived because analytical methods such as dynamic light scattering are not routinely applied as quality control procedures. The authors have developed a methodologically simple and fast procedure, based on microdialysis and image processing, that reveals the aggregation tendency of a given protein in a specific environment. Because they also show a good correlation between macroscopic aggregation and soluble aggregate formation, the microdialysis approach also permits an estimation of the occurrence of soluble aggregates.Vázquez, Esther 22010-01-0120102010-01-01Articlehttp://purl.org/coar/resource_type/c_6501AMhttp://purl.org/coar/version/c_ab4af688f83e57aainfo:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/137805https://dx.doi.org/urn:doi:10.1177/1087057110363911reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengMinisterio de Economía y Competitividad https://doi.org/10.13039/501100003329 BI02007-61194Ministerio de Ciencia e Innovación https://doi.org/10.13039/501100004837 EUI2008-03610Agència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2009/SGR-108open accesshttp://purl.org/coar/access_right/c_abf2Aquest material està protegit per drets d'autor i/o drets afins. Podeu utilitzar aquest material en funció del que permet la legislació de drets d'autor i drets afins d'aplicació al vostre cas. Per a d'altres usos heu d'obtenir permís del(s) titular(s) de drets.https://rightsstatements.org/vocab/InC/1.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:1378052026-06-06T12:50:31Z
dc.title.none.fl_str_mv Protein aggregation and soluble aggregate formation screened by a fast microdialysis assay
title Protein aggregation and soluble aggregate formation screened by a fast microdialysis assay
spellingShingle Protein aggregation and soluble aggregate formation screened by a fast microdialysis assay
Toledo-Rubio, Verónica
Protein aggregation
Nanoparticles
Dynamic light scattering
Proto-aggregates
Soluble aggregates
title_short Protein aggregation and soluble aggregate formation screened by a fast microdialysis assay
title_full Protein aggregation and soluble aggregate formation screened by a fast microdialysis assay
title_fullStr Protein aggregation and soluble aggregate formation screened by a fast microdialysis assay
title_full_unstemmed Protein aggregation and soluble aggregate formation screened by a fast microdialysis assay
title_sort Protein aggregation and soluble aggregate formation screened by a fast microdialysis assay
dc.creator.none.fl_str_mv Toledo-Rubio, Verónica
Platas, Gemma
Domingo-Espin, Joan|||0000-0001-8149-0803
Unzueta Elorza, Ugutz|||0000-0001-5119-2266
Steinkamp, Elke
Garcia-Fruitos, Elena|||0000-0001-7498-4864
Ferrer-Miralles, Neus|||0000-0003-2981-3913
Villaverde, Antonio|||0000-0002-2615-4521
author Toledo-Rubio, Verónica
author_facet Toledo-Rubio, Verónica
Platas, Gemma
Domingo-Espin, Joan|||0000-0001-8149-0803
Unzueta Elorza, Ugutz|||0000-0001-5119-2266
Steinkamp, Elke
Garcia-Fruitos, Elena|||0000-0001-7498-4864
Ferrer-Miralles, Neus|||0000-0003-2981-3913
Villaverde, Antonio|||0000-0002-2615-4521
author_role author
author2 Platas, Gemma
Domingo-Espin, Joan|||0000-0001-8149-0803
Unzueta Elorza, Ugutz|||0000-0001-5119-2266
Steinkamp, Elke
Garcia-Fruitos, Elena|||0000-0001-7498-4864
Ferrer-Miralles, Neus|||0000-0003-2981-3913
Villaverde, Antonio|||0000-0002-2615-4521
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Vázquez, Esther
dc.subject.none.fl_str_mv Protein aggregation
Nanoparticles
Dynamic light scattering
Proto-aggregates
Soluble aggregates
topic Protein aggregation
Nanoparticles
Dynamic light scattering
Proto-aggregates
Soluble aggregates
description Protein aggregation is a major obstacle in recombinant protein production as it reduces the yield of soluble polypeptides. Also, the formation of aggregates occurring in the soluble fraction is more common than formerly expected, and the prevalence of these entities might significantly affect the average quality of the soluble protein species. Usually, the formation of soluble aggregates remains unperceived because analytical methods such as dynamic light scattering are not routinely applied as quality control procedures. The authors have developed a methodologically simple and fast procedure, based on microdialysis and image processing, that reveals the aggregation tendency of a given protein in a specific environment. Because they also show a good correlation between macroscopic aggregation and soluble aggregate formation, the microdialysis approach also permits an estimation of the occurrence of soluble aggregates.
publishDate 2010
dc.date.none.fl_str_mv 2
2010-01-01
2010
2010-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
AM
http://purl.org/coar/version/c_ab4af688f83e57aa
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/137805
https://dx.doi.org/urn:doi:10.1177/1087057110363911
url https://ddd.uab.cat/record/137805
https://dx.doi.org/urn:doi:10.1177/1087057110363911
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 BI02007-61194
Ministerio de Ciencia e Innovación https://doi.org/10.13039/501100004837 EUI2008-03610
Agència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2009/SGR-108
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://rightsstatements.org/vocab/InC/1.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://rightsstatements.org/vocab/InC/1.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869419076254695424
score 15,300719

Similares en LA Referencia