Function of glutathione peroxidases in legume root nodules

39 Pags.- 3 Tabls.- 10 Figs.- The definitive version is available at: http://jxb.oxfordjournals.org/

Detalles Bibliográficos
Autores: Matamoros Galindo, Manuel Ángel, Saiz Rodríguez, Ana, Peñuelas Hortelano, Maria, Bustos-Sanmamed, Pilar, Mulet-Salort, José Miguel, Barja, M. Victoria, Rouhier, Nicolas, Moore, Marten, James, Euan Kevin, Dietz, Karl-Josef, Becana Ausejo, Manuel
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2015
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/112264
Acceso en línea:http://hdl.handle.net/10261/112264
Access Level:acceso abierto
Palabra clave:Antioxidants
glutathione peroxidases
legume nodules
Lotus japonicus
nitric oxide
reactive oxygen species
S-nitrosylation
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spelling Function of glutathione peroxidases in legume root nodulesMatamoros Galindo, Manuel ÁngelSaiz Rodríguez, AnaPeñuelas Hortelano, MariaBustos-Sanmamed, PilarMulet-Salort, José MiguelBarja, M. VictoriaRouhier, NicolasMoore, MartenJames, Euan KevinDietz, Karl-JosefBecana Ausejo, ManuelAntioxidantsglutathione peroxidaseslegume nodulesLotus japonicusnitric oxidenitric oxidereactive oxygen speciesS-nitrosylation39 Pags.- 3 Tabls.- 10 Figs.- The definitive version is available at: http://jxb.oxfordjournals.org/Glutathione peroxidases (Gpxs) are antioxidant enzymes not studied so far in legume nodules, despite the fact that reactive oxygen species are produced at different steps of the symbiosis. The function of two Gpxs that are highly expressed in nodules of the model legume Lotus japonicus was examined. Gene expression analysis, enzymatic and nitrosylation assays, yeast cell complementation, in situ mRNA hybridization, immunoelectron microscopy, and LjGpx-green fluorescent protein (GFP) fusions were used to characterize the enzymes and to localize each transcript and isoform in nodules. The LjGpx1 and LjGpx3 genes encode thioredoxin-dependent phospholipid hydroperoxidases and are differentially regulated in response to nitric oxide (NO) and hormones. LjGpx1 and LjGpx3 are nitrosylated in vitro or in plants treated with S-nitrosoglutathione (GSNO). Consistent with the modification of the peroxidatic cysteine of LjGpx3, in vitro assays demonstrated that this modification results in enzyme inhibition. The enzymes are highly expressed in the infected zone, but the LjGpx3 mRNA is also detected in the cortex and vascular bundles. LjGpx1 is localized to the plastids and nuclei, and LjGpx3 to the cytosol and endoplasmic reticulum. Based on yeast complementation experiments, both enzymes protect against oxidative stress, salt stress, and membrane damage. It is concluded that both LjGpxs perform major antioxidative functions in nodules, preventing lipid peroxidation and other oxidative processes at different subcellular sites of vascular and infected cells. The enzymes are probably involved in hormone and NO signalling, and may be regulated through nitrosylation of the peroxidatic cysteine essential for catalytic function.AS and PBS were the recipients of predoctoral (Formación de Personal Investigador) and postdoctoral (Marie Curie) contracts, respectively. We thank Martin Crespi for help with in situ RNA hybridization and Simon Avery for sharing the yeast mutant and for helpful advice. This work was supported by Ministerio de Economía y Competitividad-Fondo Europeo de Desarrollo Regional (AGL2011-24524 and AGL2014-53717-R). The UMR1136 is supported by a grant overseen by the French National Research Agency (ANR) as part of the "Investissements d'Avenir" program (ANR-11-LABX-0002-01, Lab of Excellence ARBRE). MM and KJD acknowledge support within SPP1710. The proteomic analysis was performed in the CSIC/UAB Proteomics Facility of IIBB-CSIC that belongs to ProteoRed, PRB2-ISCIII, supported by grant PT13/0001.Peer reviewedOxford University PressConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2015info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/112264reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1093/jxb/erv066Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1122642026-05-22T06:33:51Z
dc.title.none.fl_str_mv Function of glutathione peroxidases in legume root nodules
title Function of glutathione peroxidases in legume root nodules
spellingShingle Function of glutathione peroxidases in legume root nodules
Matamoros Galindo, Manuel Ángel
Antioxidants
glutathione peroxidases
legume nodules
Lotus japonicus
nitric oxide
nitric oxide
reactive oxygen species
S-nitrosylation
title_short Function of glutathione peroxidases in legume root nodules
title_full Function of glutathione peroxidases in legume root nodules
title_fullStr Function of glutathione peroxidases in legume root nodules
title_full_unstemmed Function of glutathione peroxidases in legume root nodules
title_sort Function of glutathione peroxidases in legume root nodules
dc.creator.none.fl_str_mv Matamoros Galindo, Manuel Ángel
Saiz Rodríguez, Ana
Peñuelas Hortelano, Maria
Bustos-Sanmamed, Pilar
Mulet-Salort, José Miguel
Barja, M. Victoria
Rouhier, Nicolas
Moore, Marten
James, Euan Kevin
Dietz, Karl-Josef
Becana Ausejo, Manuel
author Matamoros Galindo, Manuel Ángel
author_facet Matamoros Galindo, Manuel Ángel
Saiz Rodríguez, Ana
Peñuelas Hortelano, Maria
Bustos-Sanmamed, Pilar
Mulet-Salort, José Miguel
Barja, M. Victoria
Rouhier, Nicolas
Moore, Marten
James, Euan Kevin
Dietz, Karl-Josef
Becana Ausejo, Manuel
author_role author
author2 Saiz Rodríguez, Ana
Peñuelas Hortelano, Maria
Bustos-Sanmamed, Pilar
Mulet-Salort, José Miguel
Barja, M. Victoria
Rouhier, Nicolas
Moore, Marten
James, Euan Kevin
Dietz, Karl-Josef
Becana Ausejo, Manuel
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Antioxidants
glutathione peroxidases
legume nodules
Lotus japonicus
nitric oxide
nitric oxide
reactive oxygen species
S-nitrosylation
topic Antioxidants
glutathione peroxidases
legume nodules
Lotus japonicus
nitric oxide
nitric oxide
reactive oxygen species
S-nitrosylation
description 39 Pags.- 3 Tabls.- 10 Figs.- The definitive version is available at: http://jxb.oxfordjournals.org/
publishDate 2015
dc.date.none.fl_str_mv 2015
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/112264
url http://hdl.handle.net/10261/112264
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1093/jxb/erv066
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,81155

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