In vitro approach to refine bioconcentration and biotransformation predictions of organic persistent pollutants using cell lines

The application of the 3Rs (Replacement, Reduction, and Refinement) in animal experimentation has recently concentrated its efforts on utilizing cellular systems to predict toxicity in organisms. In this context, while refining the data obtained from cell lines, this study assesses their bioaccumula...

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Detalhes bibliográficos
Autores: De Oro Carretero, Paloma, Sanz Landaluce, Jon
Formato: artículo
Fecha de publicación:2024
País:España
Recursos:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/124131
Acesso em linha:https://hdl.handle.net/20.500.14352/124131
Access Level:acceso abierto
Palavra-chave:543
Bioconcentration
Cfree
IVIVE
Cell line
Biotransformation
In vitro
Ciencias
2301 Química Analítica
24 Ciencias de la Vida
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spelling In vitro approach to refine bioconcentration and biotransformation predictions of organic persistent pollutants using cell linesDe Oro Carretero, PalomaSanz Landaluce, Jon543BioconcentrationCfreeIVIVECell lineBiotransformationIn vitroCiencias2301 Química Analítica24 Ciencias de la VidaThe application of the 3Rs (Replacement, Reduction, and Refinement) in animal experimentation has recently concentrated its efforts on utilizing cellular systems to predict toxicity in organisms. In this context, while refining the data obtained from cell lines, this study assesses their bioaccumulation potential and various methods for extrapolating the in vitro metabolization rate constant to support modelled bioaccumulation assessments for fish and their limitations. For this purpose, the concentrations of the parent compound, phenanthrene, and its major metabolites within the cells and in the medium at various exposure times were quantified. A chemical distribution model (mass balance) was applied to calculate the concentrations of the cell-bioaccessible compounds (Cfree) based on the experimentally determined concentrations. An elevated matching was observed between the in vitro bioconcentration factor (BCF) and the in vivo BCFs reported in the literature for zebrafish liver cells (ZFL). This study demonstrates the importance of further investigating in vitro biotransformation kinetics. The results obtained with the approach developed here provide valuable information to enhance current models. Additionally, it underscores the potential of cell lines as a strategy for rapid, simple, and cost-effective predictions without the need for animal experimentation.ElsevierUniversidad Complutense de Madrid20242024-08-0220242024-08-02journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/124131reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/1241312026-06-02T12:44:21Z
dc.title.none.fl_str_mv In vitro approach to refine bioconcentration and biotransformation predictions of organic persistent pollutants using cell lines
title In vitro approach to refine bioconcentration and biotransformation predictions of organic persistent pollutants using cell lines
spellingShingle In vitro approach to refine bioconcentration and biotransformation predictions of organic persistent pollutants using cell lines
De Oro Carretero, Paloma
543
Bioconcentration
Cfree
IVIVE
Cell line
Biotransformation
In vitro
Ciencias
2301 Química Analítica
24 Ciencias de la Vida
title_short In vitro approach to refine bioconcentration and biotransformation predictions of organic persistent pollutants using cell lines
title_full In vitro approach to refine bioconcentration and biotransformation predictions of organic persistent pollutants using cell lines
title_fullStr In vitro approach to refine bioconcentration and biotransformation predictions of organic persistent pollutants using cell lines
title_full_unstemmed In vitro approach to refine bioconcentration and biotransformation predictions of organic persistent pollutants using cell lines
title_sort In vitro approach to refine bioconcentration and biotransformation predictions of organic persistent pollutants using cell lines
dc.creator.none.fl_str_mv De Oro Carretero, Paloma
Sanz Landaluce, Jon
author De Oro Carretero, Paloma
author_facet De Oro Carretero, Paloma
Sanz Landaluce, Jon
author_role author
author2 Sanz Landaluce, Jon
author2_role author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 543
Bioconcentration
Cfree
IVIVE
Cell line
Biotransformation
In vitro
Ciencias
2301 Química Analítica
24 Ciencias de la Vida
topic 543
Bioconcentration
Cfree
IVIVE
Cell line
Biotransformation
In vitro
Ciencias
2301 Química Analítica
24 Ciencias de la Vida
description The application of the 3Rs (Replacement, Reduction, and Refinement) in animal experimentation has recently concentrated its efforts on utilizing cellular systems to predict toxicity in organisms. In this context, while refining the data obtained from cell lines, this study assesses their bioaccumulation potential and various methods for extrapolating the in vitro metabolization rate constant to support modelled bioaccumulation assessments for fish and their limitations. For this purpose, the concentrations of the parent compound, phenanthrene, and its major metabolites within the cells and in the medium at various exposure times were quantified. A chemical distribution model (mass balance) was applied to calculate the concentrations of the cell-bioaccessible compounds (Cfree) based on the experimentally determined concentrations. An elevated matching was observed between the in vitro bioconcentration factor (BCF) and the in vivo BCFs reported in the literature for zebrafish liver cells (ZFL). This study demonstrates the importance of further investigating in vitro biotransformation kinetics. The results obtained with the approach developed here provide valuable information to enhance current models. Additionally, it underscores the potential of cell lines as a strategy for rapid, simple, and cost-effective predictions without the need for animal experimentation.
publishDate 2024
dc.date.none.fl_str_mv 2024
2024-08-02
2024
2024-08-02
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/124131
url https://hdl.handle.net/20.500.14352/124131
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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