Interleukin-16 is increased in obesity and alters adipogenesis and inflammation in vitro
Introduction: Obesity is a chronic condition associated with low-grade</p><p>inflammation mainly due to immune cell infiltration of white adipose tissue</p><p>(WAT). WAT is distributed into two main depots: subcutaneous WAT (sWAT)</p><p>and visceral WAT (vWAT), ea...
| Autores: | , , , , , , , , , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2024 |
| País: | España |
| Institución: | Universidad de Barcelona |
| Repositorio: | Dipòsit Digital de la UB |
| OAI Identifier: | oai:diposit.ub.edu:2445/221909 |
| Acceso en línea: | https://hdl.handle.net/2445/221909 |
| Access Level: | acceso abierto |
| Palabra clave: | Inflamació Teixit adipós Obesitat Inflammation Adipose tissues Obesity |
| Sumario: | Introduction: Obesity is a chronic condition associated with low-grade</p><p>inflammation mainly due to immune cell infiltration of white adipose tissue</p><p>(WAT). WAT is distributed into two main depots: subcutaneous WAT (sWAT)</p><p>and visceral WAT (vWAT), each with different biochemical features and metabolic</p><p>roles. Proinflammatory cytokines including interleukin (IL)-16 are secreted by</p><p>both adipocytes and infiltrated immune cells to upregulate inflammation. IL-16</p><p>has been widely studied in the peripheral proinflammatory immune response;</p><p>however, little is known about its role in adipocytes in the context of obesity.</p><p>Aim & Methods: We aimed to study the levels of IL-16 in WAT derived from sWAT</p><p>and vWAT depots of humans with obesity and the role of this cytokine in</p><p>palmitate-exposed 3T3-L1 adipocytes.</p><p>Results: The results demonstrated that IL-16 expression was higher in vWAT</p><p>compared with sWAT in individuals with obesity. In addition, IL-16 serum levels</p><p>were higher in patients with obesity compared with normal-weight individuals,</p><p>increased at 6 months after bariatric surgery, and at 12 months after surgery</p><p>decreased to levels similar to before the intervention. Our in vitro models showed</p><p>that IL-16 could modulate markers of adipogenesis (Pref1), lipid metabolism</p><p>(Plin1, Cd36, and Glut4), fibrosis (Hif1a, Col4a, Col6a, and Vegf), and inflammatory</p><p>signaling (IL6) during adipogenesis and in mature adipocytes. In addition, lipid</p><p>accumulation and glycerol release assays suggested lipolysis alteration.</p><p>Discussion: Our results suggest a potential role of IL-16 in adipogenesis, lipid and</p><p>glucose homeostasis, fibrosis, and inflammation in an obesity context. |
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