α3 β4 Nicotinic Receptors Are Components of the Secretory Machinery Clusters in Chromaffin Cells

ABSTRACT: The heteromeric assembly of α3 and β4 subunits of acetylcholine nicotinic receptors (nAChRs) seems to mediate the secretory response in bovine chromaffin cells. However, there is no information about the localization of these nAChRs in relationship with the secretory active zones in this c...

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Authors: Villanueva, José, Criado Herrero, Manuel, Giménez Molina, Yolanda, González Vélez, Virginia, Gil Gómez, Amparo|||0000-0002-7449-4205, Gutiérrez Pérez, Luis Miguel
Format: article
Publication Date:2022
Country:España
Institution:Universidad de Cantabria (UC)
Repository:UCrea Repositorio Abierto de la Universidad de Cantabria
Language:English
OAI Identifier:oai:repositorio.unican.es:10902/25712
Online Access:http://hdl.handle.net/10902/25712
Access Level:Open access
Keyword:Chromaffin cells
Exocytosis
Α3 β4 acetylcholine nicotinic receptor
SNAP-25
DBH
Secretory machinery
Particle-based methods
Modeling of calcium dynamics
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spelling α3 β4 Nicotinic Receptors Are Components of the Secretory Machinery Clusters in Chromaffin CellsVillanueva, JoséCriado Herrero, ManuelGiménez Molina, YolandaGonzález Vélez, VirginiaGil Gómez, Amparo|||0000-0002-7449-4205Gutiérrez Pérez, Luis MiguelChromaffin cellsExocytosisΑ3 β4 acetylcholine nicotinic receptorSNAP-25DBHSecretory machineryParticle-based methodsModeling of calcium dynamicsABSTRACT: The heteromeric assembly of α3 and β4 subunits of acetylcholine nicotinic receptors (nAChRs) seems to mediate the secretory response in bovine chromaffin cells. However, there is no information about the localization of these nAChRs in relationship with the secretory active zones in this cellular model. The present work presents the first evidence that, in fact, a population of these receptors is associated through the F-actin cytoskeleton with exocytotic machinery components, as detected by SNAP-25 labeling. Furthermore, we also prove that, upon stimulation, the probabilityto find α3β4 nAChRs very close to exocytotic events increases with randomized distributions, thus substantiating the clear dynamic behavior of these receptors during the secretory process. Modeling on secretory dynamics and secretory component distributions supports the idea that α3β4 nAChRcluster mobility could help with improving the efficiency of the secretory response of chromaffin cells. Our study is limited by the use of conventional confocal microscopy; in this sense, a strengthening to our conclusions could come from the use of super-resolution microscopy techniques in the near future.This work was supported by grants from the Spanish Ministerio de Economía y Competitividad (PID2020-114824GB-I00, MINECO, FEDER, UE) to L.M.G.MDPIUniversidad de Cantabria20222022-08-14journal articlehttp://purl.org/coar/resource_type/c_6501NAhttp://purl.org/coar/version/c_be7fb7dd8ff6fe43info:eu-repo/semantics/articlehttp://hdl.handle.net/10902/25712International journal of molecular sciences, 2022, 23, 2191reponame:UCrea Repositorio Abierto de la Universidad de Cantabriainstname:Universidad de Cantabria (UC)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:repositorio.unican.es:10902/257122026-06-02T12:39:31Z
dc.title.none.fl_str_mv α3 β4 Nicotinic Receptors Are Components of the Secretory Machinery Clusters in Chromaffin Cells
title α3 β4 Nicotinic Receptors Are Components of the Secretory Machinery Clusters in Chromaffin Cells
spellingShingle α3 β4 Nicotinic Receptors Are Components of the Secretory Machinery Clusters in Chromaffin Cells
Villanueva, José
Chromaffin cells
Exocytosis
Α3 β4 acetylcholine nicotinic receptor
SNAP-25
DBH
Secretory machinery
Particle-based methods
Modeling of calcium dynamics
title_short α3 β4 Nicotinic Receptors Are Components of the Secretory Machinery Clusters in Chromaffin Cells
title_full α3 β4 Nicotinic Receptors Are Components of the Secretory Machinery Clusters in Chromaffin Cells
title_fullStr α3 β4 Nicotinic Receptors Are Components of the Secretory Machinery Clusters in Chromaffin Cells
title_full_unstemmed α3 β4 Nicotinic Receptors Are Components of the Secretory Machinery Clusters in Chromaffin Cells
title_sort α3 β4 Nicotinic Receptors Are Components of the Secretory Machinery Clusters in Chromaffin Cells
dc.creator.none.fl_str_mv Villanueva, José
Criado Herrero, Manuel
Giménez Molina, Yolanda
González Vélez, Virginia
Gil Gómez, Amparo|||0000-0002-7449-4205
Gutiérrez Pérez, Luis Miguel
author Villanueva, José
author_facet Villanueva, José
Criado Herrero, Manuel
Giménez Molina, Yolanda
González Vélez, Virginia
Gil Gómez, Amparo|||0000-0002-7449-4205
Gutiérrez Pérez, Luis Miguel
author_role author
author2 Criado Herrero, Manuel
Giménez Molina, Yolanda
González Vélez, Virginia
Gil Gómez, Amparo|||0000-0002-7449-4205
Gutiérrez Pérez, Luis Miguel
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad de Cantabria
dc.subject.none.fl_str_mv Chromaffin cells
Exocytosis
Α3 β4 acetylcholine nicotinic receptor
SNAP-25
DBH
Secretory machinery
Particle-based methods
Modeling of calcium dynamics
topic Chromaffin cells
Exocytosis
Α3 β4 acetylcholine nicotinic receptor
SNAP-25
DBH
Secretory machinery
Particle-based methods
Modeling of calcium dynamics
description ABSTRACT: The heteromeric assembly of α3 and β4 subunits of acetylcholine nicotinic receptors (nAChRs) seems to mediate the secretory response in bovine chromaffin cells. However, there is no information about the localization of these nAChRs in relationship with the secretory active zones in this cellular model. The present work presents the first evidence that, in fact, a population of these receptors is associated through the F-actin cytoskeleton with exocytotic machinery components, as detected by SNAP-25 labeling. Furthermore, we also prove that, upon stimulation, the probabilityto find α3β4 nAChRs very close to exocytotic events increases with randomized distributions, thus substantiating the clear dynamic behavior of these receptors during the secretory process. Modeling on secretory dynamics and secretory component distributions supports the idea that α3β4 nAChRcluster mobility could help with improving the efficiency of the secretory response of chromaffin cells. Our study is limited by the use of conventional confocal microscopy; in this sense, a strengthening to our conclusions could come from the use of super-resolution microscopy techniques in the near future.
publishDate 2022
dc.date.none.fl_str_mv 2022
2022-08-14
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
NA
http://purl.org/coar/version/c_be7fb7dd8ff6fe43
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10902/25712
url http://hdl.handle.net/10902/25712
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv International journal of molecular sciences, 2022, 23, 2191
reponame:UCrea Repositorio Abierto de la Universidad de Cantabria
instname:Universidad de Cantabria (UC)
instname_str Universidad de Cantabria (UC)
reponame_str UCrea Repositorio Abierto de la Universidad de Cantabria
collection UCrea Repositorio Abierto de la Universidad de Cantabria
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869419010126249984
score 15,300719

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