Table_2_Association Mapping of Lathyrus sativus Disease Response to Uromyces pisi Reveals Novel Loci Underlying Partial Resistance.xlsx

Table S2. Primer sequences used for RT-qPCR analysis.

Detalles Bibliográficos
Autores: Martins, Davide Coelho, Rubiales, Diego, Vaz Patto, María Carlota
Tipo de recurso: conjunto de datos
Fecha de publicación:2022
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/330571
Acceso en línea:http://hdl.handle.net/10261/330571
Access Level:acceso abierto
Palabra clave:Genome-wide association studies
Grass pea
Partial resistance
Natural variation
Rust
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spelling Table_2_Association Mapping of Lathyrus sativus Disease Response to Uromyces pisi Reveals Novel Loci Underlying Partial Resistance.xlsxMartins, Davide CoelhoRubiales, DiegoVaz Patto, María CarlotaGenome-wide association studiesGrass peaPartial resistanceNatural variationRustTable S2. Primer sequences used for RT-qPCR analysis.Uromyces pisi ([Pers.] D.C.) Wint. is an important foliar biotrophic pathogen infecting grass pea (Lathyrus sativus L.), compromising their yield stability. To date, few efforts have been made to assess the natural variation in grass pea resistance and to identify the resistance loci operating against this pathogen, limiting its efficient breeding exploitation. To overcome this knowledge gap, the genetic architecture of grass pea resistance to U. pisi was investigated using a worldwide collection of 182 accessions through a genome-wide association approach. The response of the grass pea collection to rust infection under controlled conditions and at the seedling stage did not reveal any hypersensitive response but a continuous variation for disease severity, with the identification of promising sources of partial resistance. A panel of 5,651 high-quality single-nucleotide polymorphism (SNP) markers previously generated was used to test for SNP-trait associations, based on a mixed linear model accounting for population structure. We detected seven SNP markers significantly associated with U. pisi disease severity, suggesting that partial resistance is oligogenic. Six of the associated SNP markers were located in chromosomes 4 and 6, while the remaining SNP markers had no known chromosomal position. Through comparative mapping with the pea reference genome, a total of 19 candidate genes were proposed, encoding for leucine-rich repeat, NB-ARC domain, and TGA transcription factor family, among others. Results presented in this study provided information on the availability of partial resistance in grass pea germplasm and advanced our understanding of the molecular mechanisms of quantitative resistance to rust in grass pea. Moreover, the detected associated SNP markers constitute promising genomic targets for the development of molecular tools to assist disease resistance precision breeding.Peer reviewedFigshareConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202320232022info:eu-repo/semantics/datasethttp://purl.org/coar/resource_type/c_ddb1application/vnd.ms-excelhttp://hdl.handle.net/10261/330571reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésMartins, Davide Coelho; Rubiales, Diego; Vaz Patto, María Carlota. Association Mapping of Lathyrus sativus Disease Response to Uromyces pisi Reveals Novel Loci Underlying Partial Resistance. https://doi.org/10.3389/fpls.2022.842545 . http://hdl.handle.net/10261/286222https://doi.org/10.3389/fpls.2022.842545.s005Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3305712026-05-22T06:33:51Z
dc.title.none.fl_str_mv Table_2_Association Mapping of Lathyrus sativus Disease Response to Uromyces pisi Reveals Novel Loci Underlying Partial Resistance.xlsx
title Table_2_Association Mapping of Lathyrus sativus Disease Response to Uromyces pisi Reveals Novel Loci Underlying Partial Resistance.xlsx
spellingShingle Table_2_Association Mapping of Lathyrus sativus Disease Response to Uromyces pisi Reveals Novel Loci Underlying Partial Resistance.xlsx
Martins, Davide Coelho
Genome-wide association studies
Grass pea
Partial resistance
Natural variation
Rust
title_short Table_2_Association Mapping of Lathyrus sativus Disease Response to Uromyces pisi Reveals Novel Loci Underlying Partial Resistance.xlsx
title_full Table_2_Association Mapping of Lathyrus sativus Disease Response to Uromyces pisi Reveals Novel Loci Underlying Partial Resistance.xlsx
title_fullStr Table_2_Association Mapping of Lathyrus sativus Disease Response to Uromyces pisi Reveals Novel Loci Underlying Partial Resistance.xlsx
title_full_unstemmed Table_2_Association Mapping of Lathyrus sativus Disease Response to Uromyces pisi Reveals Novel Loci Underlying Partial Resistance.xlsx
title_sort Table_2_Association Mapping of Lathyrus sativus Disease Response to Uromyces pisi Reveals Novel Loci Underlying Partial Resistance.xlsx
dc.creator.none.fl_str_mv Martins, Davide Coelho
Rubiales, Diego
Vaz Patto, María Carlota
author Martins, Davide Coelho
author_facet Martins, Davide Coelho
Rubiales, Diego
Vaz Patto, María Carlota
author_role author
author2 Rubiales, Diego
Vaz Patto, María Carlota
author2_role author
author
dc.contributor.none.fl_str_mv Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Genome-wide association studies
Grass pea
Partial resistance
Natural variation
Rust
topic Genome-wide association studies
Grass pea
Partial resistance
Natural variation
Rust
description Table S2. Primer sequences used for RT-qPCR analysis.
publishDate 2022
dc.date.none.fl_str_mv 2022
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/dataset
http://purl.org/coar/resource_type/c_ddb1
format dataset
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/330571
url http://hdl.handle.net/10261/330571
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Martins, Davide Coelho; Rubiales, Diego; Vaz Patto, María Carlota. Association Mapping of Lathyrus sativus Disease Response to Uromyces pisi Reveals Novel Loci Underlying Partial Resistance. https://doi.org/10.3389/fpls.2022.842545 . http://hdl.handle.net/10261/286222
https://doi.org/10.3389/fpls.2022.842545.s005
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/vnd.ms-excel
dc.publisher.none.fl_str_mv Figshare
publisher.none.fl_str_mv Figshare
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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