A faecal microbiota signature with high specificity for pancreatic cancer

Cancer prevention; Intestinal microbiology; Pancreatic tumours

Detalles Bibliográficos
Autores: Kartal, Ece, Schmidt, Thomas S.B., Molina-Montes, Esther, Rodriguez-Perales, Sandra, Wirbel, Jakob, Maistrenko, Oleksandr, Molero Richard, Francesc Xavier
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:11351/7900
Acceso en línea:https://hdl.handle.net/11351/7900
http://hdl.handle.net/11351/7900
Access Level:acceso abierto
Palabra clave:Pàncrees - Càncer - Diagnòstic
Intestins - Microbiologia
DISEASES::Neoplasms::Neoplasms by Site::Digestive System Neoplasms::Pancreatic Neoplasms
Other subheadings::Other subheadings::/diagnosis
ENFERMEDADES::neoplasias::neoplasias por localización::neoplasias del sistema digestivo::neoplasias pancreáticas
Otros calificadores::Otros calificadores::/diagnóstico
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oai_identifier_str oai:recercat.cat:11351/7900
network_acronym_str ES
network_name_str España
repository_id_str
dc.title.none.fl_str_mv A faecal microbiota signature with high specificity for pancreatic cancer
title A faecal microbiota signature with high specificity for pancreatic cancer
spellingShingle A faecal microbiota signature with high specificity for pancreatic cancer
Kartal, Ece
Pàncrees - Càncer - Diagnòstic
Intestins - Microbiologia
DISEASES::Neoplasms::Neoplasms by Site::Digestive System Neoplasms::Pancreatic Neoplasms
Other subheadings::Other subheadings::/diagnosis
ENFERMEDADES::neoplasias::neoplasias por localización::neoplasias del sistema digestivo::neoplasias pancreáticas
Otros calificadores::Otros calificadores::/diagnóstico
title_short A faecal microbiota signature with high specificity for pancreatic cancer
title_full A faecal microbiota signature with high specificity for pancreatic cancer
title_fullStr A faecal microbiota signature with high specificity for pancreatic cancer
title_full_unstemmed A faecal microbiota signature with high specificity for pancreatic cancer
title_sort A faecal microbiota signature with high specificity for pancreatic cancer
dc.creator.none.fl_str_mv Kartal, Ece
Schmidt, Thomas S.B.
Molina-Montes, Esther
Rodriguez-Perales, Sandra
Wirbel, Jakob
Maistrenko, Oleksandr
Molero Richard, Francesc Xavier
author Kartal, Ece
author_facet Kartal, Ece
Schmidt, Thomas S.B.
Molina-Montes, Esther
Rodriguez-Perales, Sandra
Wirbel, Jakob
Maistrenko, Oleksandr
Molero Richard, Francesc Xavier
author_role author
author2 Schmidt, Thomas S.B.
Molina-Montes, Esther
Rodriguez-Perales, Sandra
Wirbel, Jakob
Maistrenko, Oleksandr
Molero Richard, Francesc Xavier
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Institut Català de la Salut
[Kartal E, Wirbel J] Structural and Computational Biology Unit, European Molecular Biology Laboratory, Heidelberg, Germany. Collaboration for joint PhD degree, European Molecular Biology Laboratory and Heidelberg University, Heidelberg, Germany. [Schmidt TSB, Maistrenko OM] Structural and Computational Biology Unit, European Molecular Biology Laboratory, Heidelberg, Germany. [Molina-Montes E] Genetic and Molecular Epidemiology Group, Spanish National Cancer Research Centre (CNIO), Madrid, Spain. Centro de Investigación Biomédica en Red de Oncología (CIBERONC), Madrid, Spain. [Rodríguez-Perales S] Centro de Investigación Biomédica en Red de Oncología (CIBERONC), Madrid, Spain. Molecular Cytogenetics Unit, Spanish National Cancer Research Centre (CNIO), Madrid, Spain. [Molero X] Vall d’Hebron Hospital Universitari, Barcelona, Spain. Vall d’Hebron Institut de Recerca (VHIR), Barcelona, Spain. Universitat Autònoma de Barcelona, Bellaterra, Spain. Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBEREHD), Madrid, Spain
Vall d'Hebron Barcelona Hospital Campus
dc.subject.none.fl_str_mv Pàncrees - Càncer - Diagnòstic
Intestins - Microbiologia
DISEASES::Neoplasms::Neoplasms by Site::Digestive System Neoplasms::Pancreatic Neoplasms
Other subheadings::Other subheadings::/diagnosis
ENFERMEDADES::neoplasias::neoplasias por localización::neoplasias del sistema digestivo::neoplasias pancreáticas
Otros calificadores::Otros calificadores::/diagnóstico
topic Pàncrees - Càncer - Diagnòstic
Intestins - Microbiologia
DISEASES::Neoplasms::Neoplasms by Site::Digestive System Neoplasms::Pancreatic Neoplasms
Other subheadings::Other subheadings::/diagnosis
ENFERMEDADES::neoplasias::neoplasias por localización::neoplasias del sistema digestivo::neoplasias pancreáticas
Otros calificadores::Otros calificadores::/diagnóstico
description Cancer prevention; Intestinal microbiology; Pancreatic tumours
publishDate 2022
dc.date.none.fl_str_mv 2022
2022
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/11351/7900
http://hdl.handle.net/11351/7900
url https://hdl.handle.net/11351/7900
http://hdl.handle.net/11351/7900
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Gut;71(7)
http://dx.doi.org/10.1136/gutjnl-2021-324755
dc.rights.none.fl_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv BMJ
publisher.none.fl_str_mv BMJ
dc.source.none.fl_str_mv Scientia
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869419005692870656
spelling A faecal microbiota signature with high specificity for pancreatic cancerKartal, EceSchmidt, Thomas S.B.Molina-Montes, EstherRodriguez-Perales, SandraWirbel, JakobMaistrenko, OleksandrMolero Richard, Francesc XavierPàncrees - Càncer - DiagnòsticIntestins - MicrobiologiaDISEASES::Neoplasms::Neoplasms by Site::Digestive System Neoplasms::Pancreatic NeoplasmsOther subheadings::Other subheadings::/diagnosisENFERMEDADES::neoplasias::neoplasias por localización::neoplasias del sistema digestivo::neoplasias pancreáticasOtros calificadores::Otros calificadores::/diagnósticoCancer prevention; Intestinal microbiology; Pancreatic tumoursPrevenció del càncer; Microbiologia intestinal; Tumors pancreàticsPrevención de cáncer; Microbiología intestinal; Tumores pancreáticosBackground Recent evidence suggests a role for the microbiome in pancreatic ductal adenocarcinoma (PDAC) aetiology and progression. Objective To explore the faecal and salivary microbiota as potential diagnostic biomarkers. Methods We applied shotgun metagenomic and 16S rRNA amplicon sequencing to samples from a Spanish case–control study (n=136), including 57 cases, 50 controls, and 29 patients with chronic pancreatitis in the discovery phase, and from a German case–control study (n=76), in the validation phase. Results Faecal metagenomic classifiers performed much better than saliva-based classifiers and identified patients with PDAC with an accuracy of up to 0.84 area under the receiver operating characteristic curve (AUROC) based on a set of 27 microbial species, with consistent accuracy across early and late disease stages. Performance further improved to up to 0.94 AUROC when we combined our microbiome-based predictions with serum levels of carbohydrate antigen (CA) 19–9, the only current non-invasive, Food and Drug Administration approved, low specificity PDAC diagnostic biomarker. Furthermore, a microbiota-based classification model confined to PDAC-enriched species was highly disease-specific when validated against 25 publicly available metagenomic study populations for various health conditions (n=5792). Both microbiome-based models had a high prediction accuracy on a German validation population (n=76). Several faecal PDAC marker species were detectable in pancreatic tumour and non-tumour tissue using 16S rRNA sequencing and fluorescence in situ hybridisation. Conclusion Taken together, our results indicate that non-invasive, robust and specific faecal microbiota-based screening for the early detection of PDAC is feasible.We acknowledge funding from EMBL, CNIO, World Cancer Research (#15–0391), the European Research Council (ERC-AdG-669830 MicrobioS), the BMBF-funded Heidelberg CenterCentre for Human Bioinformatics (HD-HuB) within the German Network for Bioinformatics Infrastructure (de.NBI #031A537B), Fondo de Investigaciones Sanitarias (FIS), Instituto de Salud Carlos III-FEDER, Spain (grant numbers PI15/01573, PI18/01347, FIS PI17/02303); Red Temática de Investigación Cooperativa en Cáncer, Spain (grant numbers RD12/0036/0034, RD12/0036/0050, RD12/0036/0073); III beca Carmen Delgado/Miguel Pérez-Mateo de AESPANC-ACANPAN; EU-6FP Integrated Project (#018771-MOLDIAG-PACA); EU-FP7-HEALTH (#259737-CANCERALIA). Funders had no involvement in the study design, patient enrolment, analysis, manuscript writing or reviewing.BMJInstitut Català de la Salut[Kartal E, Wirbel J] Structural and Computational Biology Unit, European Molecular Biology Laboratory, Heidelberg, Germany. Collaboration for joint PhD degree, European Molecular Biology Laboratory and Heidelberg University, Heidelberg, Germany. [Schmidt TSB, Maistrenko OM] Structural and Computational Biology Unit, European Molecular Biology Laboratory, Heidelberg, Germany. [Molina-Montes E] Genetic and Molecular Epidemiology Group, Spanish National Cancer Research Centre (CNIO), Madrid, Spain. Centro de Investigación Biomédica en Red de Oncología (CIBERONC), Madrid, Spain. [Rodríguez-Perales S] Centro de Investigación Biomédica en Red de Oncología (CIBERONC), Madrid, Spain. Molecular Cytogenetics Unit, Spanish National Cancer Research Centre (CNIO), Madrid, Spain. [Molero X] Vall d’Hebron Hospital Universitari, Barcelona, Spain. Vall d’Hebron Institut de Recerca (VHIR), Barcelona, Spain. Universitat Autònoma de Barcelona, Bellaterra, Spain. Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBEREHD), Madrid, SpainVall d'Hebron Barcelona Hospital Campus202220222022info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/11351/7900http://hdl.handle.net/11351/7900Scientiareponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésGut;71(7)http://dx.doi.org/10.1136/gutjnl-2021-324755Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:11351/79002026-05-29T05:05:01Z
score 15.812429

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