HOP co-chaperones contribute to GA signaling by promoting the accumulation of the F-box protein SNE in Arabidopsis

14 Pág.

Detalles Bibliográficos
Autores: Mangano, Silvina, Muñoz, Alfonso, Fernández-Calvino, L., Castellano Moreno, María Mar
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/309717
Acceso en línea:http://hdl.handle.net/10261/309717
https://api.elsevier.com/content/abstract/scopus_id/85147342289
Access Level:acceso abierto
Palabra clave:HSP70-HSP90 organizing protein
RGA
Protein folding
Protein stability
Seed germination
Thermomorphogenesis
id ES_c58487fdfad4e26e4b68d0779b965c6c
oai_identifier_str oai:digital.csic.es:10261/309717
network_acronym_str ES
network_name_str España
repository_id_str
spelling HOP co-chaperones contribute to GA signaling by promoting the accumulation of the F-box protein SNE in ArabidopsisMangano, SilvinaMuñoz, AlfonsoFernández-Calvino, L.Castellano Moreno, María MarHSP70-HSP90 organizing proteinRGAProtein foldingProtein stabilitySeed germinationThermomorphogenesis14 Pág.Gibberellins (GAs) play important roles in multiple developmental processes and in plant response to the environment. Within the GA pathway, a central regulatory step relies on GA-dependent degradation of the DELLA transcriptional regulators. Nevertheless, the relevance of the stability of other key proteins in this pathway, such as SLY1 and SNE (the F-box proteins involved in DELLA degradation), remains unknown. Here, we take advantage of mutants in the HSP70-HSP90 organizing protein (HOP) co-chaperones and reveal that these proteins contribute to the accumulation of SNE in Arabidopsis. Indeed, HOP proteins, along with HSP90 and HSP70, interact in vivo with SNE, and SNE accumulation is significantly reduced in the hop mutants. Concomitantly, greater accumulation of the DELLA protein RGA is observed in these plants. In agreement with these molecular phenotypes, hop mutants show a hypersensitive response to the GA inhibitor paclobutrazol and display a partial response to the ectopic addition of GA when GA-regulated processes are assayed. These mutants also display different phenotypes associated with alterations in the GA pathway, such as reduced germination rate, delayed bolting, and reduced hypocotyl elongation in response to warm temperatures. Remarkably, ectopic overexpression of SNE reverts the delay in germination and the thermally dependent hypocotyl elongation defect of the hop1 hop2 hop3 mutant, revealing that SNE accumulation is the key aspect of the hop mutant phenotypes. Together, these data reveal a pivotal role for HOP in SNE accumulation and GA signaling.This work is supported by the project RTI2018-095946-B-I00 and PID2021-126956OB-I00 from MICIU and by ‘‘Severo Ochoa Programme for Centres of Excellence in R&D’’ from the Agencia Estatal de Investigacion of Spain (grants SEV-2016-0672 and CEX2020-000999-S to the CBGP). In the frame of this latter program, S.M. was supported with post doctoral contracts. We also acknowledge the grant ‘‘Recualificacio´ n del profesorado universitario’’ from the Ministerio de Universidades to A.M. (UCO)With funding from the Spanish government through the ‘Severo Ochoa Centre of Excellence’ accreditation (CEX2020‐000999‐S)Peer reviewedCell PressMinisterio de Ciencia, Innovación y Universidades (España)Agencia Estatal de Investigación (España)Muñoz, Alfonso [0000-0001-6399-3853]Fernández-Calvino, L.[0000-0003-0988-401X]Castellano Moreno, María Mar [0000-0003-0339-4247]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202320232023info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/309717https://api.elsevier.com/content/abstract/scopus_id/85147342289reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-095946-B-I00info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2021-126956OB-I00info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/CEX2020‐000999‐SCentro de Biotecnología y Genómica de Plantas (CBGP)https://doi.org/10.1016/j.xplc.2023.100517Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3097172026-05-22T06:33:51Z
dc.title.none.fl_str_mv HOP co-chaperones contribute to GA signaling by promoting the accumulation of the F-box protein SNE in Arabidopsis
title HOP co-chaperones contribute to GA signaling by promoting the accumulation of the F-box protein SNE in Arabidopsis
spellingShingle HOP co-chaperones contribute to GA signaling by promoting the accumulation of the F-box protein SNE in Arabidopsis
Mangano, Silvina
HSP70-HSP90 organizing protein
RGA
Protein folding
Protein stability
Seed germination
Thermomorphogenesis
title_short HOP co-chaperones contribute to GA signaling by promoting the accumulation of the F-box protein SNE in Arabidopsis
title_full HOP co-chaperones contribute to GA signaling by promoting the accumulation of the F-box protein SNE in Arabidopsis
title_fullStr HOP co-chaperones contribute to GA signaling by promoting the accumulation of the F-box protein SNE in Arabidopsis
title_full_unstemmed HOP co-chaperones contribute to GA signaling by promoting the accumulation of the F-box protein SNE in Arabidopsis
title_sort HOP co-chaperones contribute to GA signaling by promoting the accumulation of the F-box protein SNE in Arabidopsis
dc.creator.none.fl_str_mv Mangano, Silvina
Muñoz, Alfonso
Fernández-Calvino, L.
Castellano Moreno, María Mar
author Mangano, Silvina
author_facet Mangano, Silvina
Muñoz, Alfonso
Fernández-Calvino, L.
Castellano Moreno, María Mar
author_role author
author2 Muñoz, Alfonso
Fernández-Calvino, L.
Castellano Moreno, María Mar
author2_role author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia, Innovación y Universidades (España)
Agencia Estatal de Investigación (España)
Muñoz, Alfonso [0000-0001-6399-3853]
Fernández-Calvino, L.[0000-0003-0988-401X]
Castellano Moreno, María Mar [0000-0003-0339-4247]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv HSP70-HSP90 organizing protein
RGA
Protein folding
Protein stability
Seed germination
Thermomorphogenesis
topic HSP70-HSP90 organizing protein
RGA
Protein folding
Protein stability
Seed germination
Thermomorphogenesis
description 14 Pág.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/309717
https://api.elsevier.com/content/abstract/scopus_id/85147342289
url http://hdl.handle.net/10261/309717
https://api.elsevier.com/content/abstract/scopus_id/85147342289
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-095946-B-I00
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2021-126956OB-I00
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/CEX2020‐000999‐S
Centro de Biotecnología y Genómica de Plantas (CBGP)
https://doi.org/10.1016/j.xplc.2023.100517
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Cell Press
publisher.none.fl_str_mv Cell Press
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869418995354959872
score 15,812429

Similares en LA Referencia