Antigen-functionalized turnip mosaic virus nanoparticles increase antibody sensing in saliva. A case study with SARS-CoV-2 RBD

Nanoparticles derived from plant viruses play an important role in nanomedicine due to their biocompatibility, self-assembly and easily-modifiable surface. In this study, we developed a novel platform for increasing antibody sensing using viral nanoparticles derived from turnip mosaic virus (TuMV) f...

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Detalles Bibliográficos
Autores: Truchado Martín, Daniel Alejandro, Medrano Arranz, Carlos, Rincón, Sara, Zurita, Lucía, Ponz, Fernando
Tipo de recurso: artículo
Fecha de publicación:2024
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/103201
Acceso en línea:https://hdl.handle.net/20.500.14352/103201
Access Level:acceso abierto
Palabra clave:577.27
579.61
Turnip mosaic virus
SARS-CoV-2
VLP
Antibody sensing
Saliva
IgA
Microbiología (Biología)
Inmunología
Biotecnología
2414 Microbiología
2420 Virología
2412 Inmunología
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oai_identifier_str oai:docta.ucm.es:20.500.14352/103201
network_acronym_str ES
network_name_str España
repository_id_str
spelling Antigen-functionalized turnip mosaic virus nanoparticles increase antibody sensing in saliva. A case study with SARS-CoV-2 RBDTruchado Martín, Daniel AlejandroMedrano Arranz, CarlosRincón, SaraZurita, LucíaPonz, Fernando577.27579.61Turnip mosaic virusSARS-CoV-2VLPAntibody sensingSalivaIgAMicrobiología (Biología)InmunologíaBiotecnología2414 Microbiología2420 Virología2412 InmunologíaNanoparticles derived from plant viruses play an important role in nanomedicine due to their biocompatibility, self-assembly and easily-modifiable surface. In this study, we developed a novel platform for increasing antibody sensing using viral nanoparticles derived from turnip mosaic virus (TuMV) functionalized with SARS-CoV-2 receptor binding domain (RBD) through three different methods: chemical conjugation, gene fusion and the SpyTag/SpyCatcher technology. Even though gene fusion turned out to be unsuccessful, the other two constructs were proven to significantly increase antibody sensing when tested with saliva of patients with different infection and vaccination status to SARS-CoV-2. Our findings show the high potential of TuMV nanoparticles in the fields of diagnostics and immunodetection, being especially attractive for the development of novel antibody sensing devices.ElsevierUniversidad Complutense de Madrid20242024-04-0720242024-04-07journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/103201reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/1032012026-06-02T12:44:21Z
dc.title.none.fl_str_mv Antigen-functionalized turnip mosaic virus nanoparticles increase antibody sensing in saliva. A case study with SARS-CoV-2 RBD
title Antigen-functionalized turnip mosaic virus nanoparticles increase antibody sensing in saliva. A case study with SARS-CoV-2 RBD
spellingShingle Antigen-functionalized turnip mosaic virus nanoparticles increase antibody sensing in saliva. A case study with SARS-CoV-2 RBD
Truchado Martín, Daniel Alejandro
577.27
579.61
Turnip mosaic virus
SARS-CoV-2
VLP
Antibody sensing
Saliva
IgA
Microbiología (Biología)
Inmunología
Biotecnología
2414 Microbiología
2420 Virología
2412 Inmunología
title_short Antigen-functionalized turnip mosaic virus nanoparticles increase antibody sensing in saliva. A case study with SARS-CoV-2 RBD
title_full Antigen-functionalized turnip mosaic virus nanoparticles increase antibody sensing in saliva. A case study with SARS-CoV-2 RBD
title_fullStr Antigen-functionalized turnip mosaic virus nanoparticles increase antibody sensing in saliva. A case study with SARS-CoV-2 RBD
title_full_unstemmed Antigen-functionalized turnip mosaic virus nanoparticles increase antibody sensing in saliva. A case study with SARS-CoV-2 RBD
title_sort Antigen-functionalized turnip mosaic virus nanoparticles increase antibody sensing in saliva. A case study with SARS-CoV-2 RBD
dc.creator.none.fl_str_mv Truchado Martín, Daniel Alejandro
Medrano Arranz, Carlos
Rincón, Sara
Zurita, Lucía
Ponz, Fernando
author Truchado Martín, Daniel Alejandro
author_facet Truchado Martín, Daniel Alejandro
Medrano Arranz, Carlos
Rincón, Sara
Zurita, Lucía
Ponz, Fernando
author_role author
author2 Medrano Arranz, Carlos
Rincón, Sara
Zurita, Lucía
Ponz, Fernando
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 577.27
579.61
Turnip mosaic virus
SARS-CoV-2
VLP
Antibody sensing
Saliva
IgA
Microbiología (Biología)
Inmunología
Biotecnología
2414 Microbiología
2420 Virología
2412 Inmunología
topic 577.27
579.61
Turnip mosaic virus
SARS-CoV-2
VLP
Antibody sensing
Saliva
IgA
Microbiología (Biología)
Inmunología
Biotecnología
2414 Microbiología
2420 Virología
2412 Inmunología
description Nanoparticles derived from plant viruses play an important role in nanomedicine due to their biocompatibility, self-assembly and easily-modifiable surface. In this study, we developed a novel platform for increasing antibody sensing using viral nanoparticles derived from turnip mosaic virus (TuMV) functionalized with SARS-CoV-2 receptor binding domain (RBD) through three different methods: chemical conjugation, gene fusion and the SpyTag/SpyCatcher technology. Even though gene fusion turned out to be unsuccessful, the other two constructs were proven to significantly increase antibody sensing when tested with saliva of patients with different infection and vaccination status to SARS-CoV-2. Our findings show the high potential of TuMV nanoparticles in the fields of diagnostics and immunodetection, being especially attractive for the development of novel antibody sensing devices.
publishDate 2024
dc.date.none.fl_str_mv 2024
2024-04-07
2024
2024-04-07
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/103201
url https://hdl.handle.net/20.500.14352/103201
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300719

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