Combination of RT-PCR and proteomics for the identification of Crimean-Congo hemorrhagic fever virus in ticks

Crimean-Congo hemorrhagic fever (CCHF) is an emerging tick-borne zoonotic disease caused by the CCHF virus (CCHFV). In this study, an experimental approach combining RT-PCR and proteomics was used for the identification and characterization of CCHFV in 106 ticks from 7 species that were collected fr...

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Autores: Fuente García, José de Jesús de la, García Fernández de Mera, María Isabel, Chaligiannis, Ilias, Hernández Jarguín, Angélica María, Villar Rayo, Margarita María, Mateos Hernández, Lourdes, Papa, Anna, Sotirak, Smaragda, Ruiz Fons, José Francisco, Cabezas Cruz, Alejandro, Gortázar Schmidt, Ramón Christian
Tipo de recurso: artículo
Fecha de publicación:2017
País:España
Institución:Universidad de Castilla-La Mancha
Repositorio:RUIdeRA. Repositorio Institucional de la UCLM
OAI Identifier:oai:ruidera.uclm.es:10578/16819
Acceso en línea:http://hdl.handle.net/10578/16819
Access Level:acceso abierto
Palabra clave:Enfermedades infecciosas
Genética
Virología
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spelling Combination of RT-PCR and proteomics for the identification of Crimean-Congo hemorrhagic fever virus in ticksFuente García, José de Jesús de laGarcía Fernández de Mera, María IsabelChaligiannis, IliasHernández Jarguín, Angélica MaríaVillar Rayo, Margarita MaríaMateos Hernández, LourdesPapa, AnnaSotirak, SmaragdaRuiz Fons, José FranciscoCabezas Cruz, AlejandroGortázar Schmidt, Ramón ChristianEnfermedades infecciosasGenéticaVirologíaCrimean-Congo hemorrhagic fever (CCHF) is an emerging tick-borne zoonotic disease caused by the CCHF virus (CCHFV). In this study, an experimental approach combining RT-PCR and proteomics was used for the identification and characterization of CCHFV in 106 ticks from 7 species that were collected from small ruminants in Greece. The methodological approach included an initial screening for CCHFV by RT-PCR followed by proteomics analysis of positive and control negative tick samples. This novel approach allowed the identification of CCHFV-positive ticks and provided additional information to corroborate the RTPCR findings using a different approach. Two ticks, Dermacentor marginatus and Haemaphysalis parva collected from a goat and a sheep, respectively were positive for CCHFV. The sequences for CCHFV RNA segments S and L were characterized by RT-PCR and proteomics analysis of tick samples, respectively. These results showed the possibility of combining analyses at the RNA and protein levels using RT-PCR and proteomics for the characterization of CCHFV in ticks. The results supported that the CCHFV identified in ticks are genetic variants of the AP92 strain. Although the AP92-like strains probably do not represent a high risk of CCHF to the population, the circulation of genetically diverse CCHFV strains could potentially result in the appearance of novel viral genotypes with increased pathogenicity and fitness.Elsevier201820182017info:eu-repo/semantics/articletext/plainapplication/pdfhttp://hdl.handle.net/10578/16819reponame:RUIdeRA. Repositorio Institucional de la UCLMinstname:Universidad de Castilla-La ManchaInglésinfo:eu-repo/semantics/openAccessoai:ruidera.uclm.es:10578/168192026-05-27T07:36:41Z
dc.title.none.fl_str_mv Combination of RT-PCR and proteomics for the identification of Crimean-Congo hemorrhagic fever virus in ticks
title Combination of RT-PCR and proteomics for the identification of Crimean-Congo hemorrhagic fever virus in ticks
spellingShingle Combination of RT-PCR and proteomics for the identification of Crimean-Congo hemorrhagic fever virus in ticks
Fuente García, José de Jesús de la
Enfermedades infecciosas
Genética
Virología
title_short Combination of RT-PCR and proteomics for the identification of Crimean-Congo hemorrhagic fever virus in ticks
title_full Combination of RT-PCR and proteomics for the identification of Crimean-Congo hemorrhagic fever virus in ticks
title_fullStr Combination of RT-PCR and proteomics for the identification of Crimean-Congo hemorrhagic fever virus in ticks
title_full_unstemmed Combination of RT-PCR and proteomics for the identification of Crimean-Congo hemorrhagic fever virus in ticks
title_sort Combination of RT-PCR and proteomics for the identification of Crimean-Congo hemorrhagic fever virus in ticks
dc.creator.none.fl_str_mv Fuente García, José de Jesús de la
García Fernández de Mera, María Isabel
Chaligiannis, Ilias
Hernández Jarguín, Angélica María
Villar Rayo, Margarita María
Mateos Hernández, Lourdes
Papa, Anna
Sotirak, Smaragda
Ruiz Fons, José Francisco
Cabezas Cruz, Alejandro
Gortázar Schmidt, Ramón Christian
author Fuente García, José de Jesús de la
author_facet Fuente García, José de Jesús de la
García Fernández de Mera, María Isabel
Chaligiannis, Ilias
Hernández Jarguín, Angélica María
Villar Rayo, Margarita María
Mateos Hernández, Lourdes
Papa, Anna
Sotirak, Smaragda
Ruiz Fons, José Francisco
Cabezas Cruz, Alejandro
Gortázar Schmidt, Ramón Christian
author_role author
author2 García Fernández de Mera, María Isabel
Chaligiannis, Ilias
Hernández Jarguín, Angélica María
Villar Rayo, Margarita María
Mateos Hernández, Lourdes
Papa, Anna
Sotirak, Smaragda
Ruiz Fons, José Francisco
Cabezas Cruz, Alejandro
Gortázar Schmidt, Ramón Christian
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Enfermedades infecciosas
Genética
Virología
topic Enfermedades infecciosas
Genética
Virología
description Crimean-Congo hemorrhagic fever (CCHF) is an emerging tick-borne zoonotic disease caused by the CCHF virus (CCHFV). In this study, an experimental approach combining RT-PCR and proteomics was used for the identification and characterization of CCHFV in 106 ticks from 7 species that were collected from small ruminants in Greece. The methodological approach included an initial screening for CCHFV by RT-PCR followed by proteomics analysis of positive and control negative tick samples. This novel approach allowed the identification of CCHFV-positive ticks and provided additional information to corroborate the RTPCR findings using a different approach. Two ticks, Dermacentor marginatus and Haemaphysalis parva collected from a goat and a sheep, respectively were positive for CCHFV. The sequences for CCHFV RNA segments S and L were characterized by RT-PCR and proteomics analysis of tick samples, respectively. These results showed the possibility of combining analyses at the RNA and protein levels using RT-PCR and proteomics for the characterization of CCHFV in ticks. The results supported that the CCHFV identified in ticks are genetic variants of the AP92 strain. Although the AP92-like strains probably do not represent a high risk of CCHF to the population, the circulation of genetically diverse CCHFV strains could potentially result in the appearance of novel viral genotypes with increased pathogenicity and fitness.
publishDate 2017
dc.date.none.fl_str_mv 2017
2018
2018
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10578/16819
url http://hdl.handle.net/10578/16819
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/plain
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:RUIdeRA. Repositorio Institucional de la UCLM
instname:Universidad de Castilla-La Mancha
instname_str Universidad de Castilla-La Mancha
reponame_str RUIdeRA. Repositorio Institucional de la UCLM
collection RUIdeRA. Repositorio Institucional de la UCLM
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.300719

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