The Janus Role of Adhesion in Chondrogenesis

Tackling the first stages of the chondrogenic commitment is essential to drive chondrogenic differentiation to healthy hyaline cartilage and minimize hypertrophy. During chondrogenesis, the extracellular matrix continuously evolves, adapting to the tissue adhesive requirements at each stage. Here, w...

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Detalles Bibliográficos
Autores: Casanellas, Ignasi, Lagunas, Anna, Vida, Yolanda, Pérez Inestrosa, Ezequiel, Andrades, José A., Becerra, José, Samitier i Martí, Josep
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/174970
Acceso en línea:https://hdl.handle.net/2445/174970
Access Level:acceso abierto
Palabra clave:Condrogènesi
Dendrímers
Malalties dels cartílags
Chondrogenesis
Dendrimers
Cartilage diseases
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spelling The Janus Role of Adhesion in ChondrogenesisCasanellas, IgnasiLagunas, AnnaVida, YolandaPérez Inestrosa, EzequielAndrades, José A.Becerra, JoséSamitier i Martí, JosepCondrogènesiDendrímersMalalties dels cartílagsChondrogenesisDendrimersCartilage diseasesTackling the first stages of the chondrogenic commitment is essential to drive chondrogenic differentiation to healthy hyaline cartilage and minimize hypertrophy. During chondrogenesis, the extracellular matrix continuously evolves, adapting to the tissue adhesive requirements at each stage. Here, we take advantage of previously developed nanopatterns, in which local surface adhesiveness can be precisely tuned, to investigate its effects on prechondrogenic condensation. Fluorescence live cell imaging, immunostaining, confocal microscopy and PCR analysis are used to follow the condensation process on the nanopatterns. Cell tracking parameters, condensate morphology, cell-cell interactions, mechanotransduction and chondrogenic commitment are evaluated in response to local surface adhesiveness. Results show that only condensates on the nanopatterns of high local surface adhesiveness are stable in culture and able to enter the chondrogenic pathway, thus highlighting the importance of controlling cell-substrate adhesion in the tissue engineering strategies for cartilage repair.MDPI2020info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/174970Articles publicats en revistes (Enginyeria Electrònica i Biomèdica)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.3390/ijms21155269International Journal of Molecular Sciences, 2020, vol. 21(15), num. 5269https://doi.org/10.3390/ijms21155269cc-by (c) Casanellas, Ignasi et al., 2020http://creativecommons.org/licenses/by/3.0/esinfo:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1749702026-05-27T06:46:51Z
dc.title.none.fl_str_mv The Janus Role of Adhesion in Chondrogenesis
title The Janus Role of Adhesion in Chondrogenesis
spellingShingle The Janus Role of Adhesion in Chondrogenesis
Casanellas, Ignasi
Condrogènesi
Dendrímers
Malalties dels cartílags
Chondrogenesis
Dendrimers
Cartilage diseases
title_short The Janus Role of Adhesion in Chondrogenesis
title_full The Janus Role of Adhesion in Chondrogenesis
title_fullStr The Janus Role of Adhesion in Chondrogenesis
title_full_unstemmed The Janus Role of Adhesion in Chondrogenesis
title_sort The Janus Role of Adhesion in Chondrogenesis
dc.creator.none.fl_str_mv Casanellas, Ignasi
Lagunas, Anna
Vida, Yolanda
Pérez Inestrosa, Ezequiel
Andrades, José A.
Becerra, José
Samitier i Martí, Josep
author Casanellas, Ignasi
author_facet Casanellas, Ignasi
Lagunas, Anna
Vida, Yolanda
Pérez Inestrosa, Ezequiel
Andrades, José A.
Becerra, José
Samitier i Martí, Josep
author_role author
author2 Lagunas, Anna
Vida, Yolanda
Pérez Inestrosa, Ezequiel
Andrades, José A.
Becerra, José
Samitier i Martí, Josep
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Condrogènesi
Dendrímers
Malalties dels cartílags
Chondrogenesis
Dendrimers
Cartilage diseases
topic Condrogènesi
Dendrímers
Malalties dels cartílags
Chondrogenesis
Dendrimers
Cartilage diseases
description Tackling the first stages of the chondrogenic commitment is essential to drive chondrogenic differentiation to healthy hyaline cartilage and minimize hypertrophy. During chondrogenesis, the extracellular matrix continuously evolves, adapting to the tissue adhesive requirements at each stage. Here, we take advantage of previously developed nanopatterns, in which local surface adhesiveness can be precisely tuned, to investigate its effects on prechondrogenic condensation. Fluorescence live cell imaging, immunostaining, confocal microscopy and PCR analysis are used to follow the condensation process on the nanopatterns. Cell tracking parameters, condensate morphology, cell-cell interactions, mechanotransduction and chondrogenic commitment are evaluated in response to local surface adhesiveness. Results show that only condensates on the nanopatterns of high local surface adhesiveness are stable in culture and able to enter the chondrogenic pathway, thus highlighting the importance of controlling cell-substrate adhesion in the tissue engineering strategies for cartilage repair.
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/174970
url https://hdl.handle.net/2445/174970
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.3390/ijms21155269
International Journal of Molecular Sciences, 2020, vol. 21(15), num. 5269
https://doi.org/10.3390/ijms21155269
dc.rights.none.fl_str_mv cc-by (c) Casanellas, Ignasi et al., 2020
http://creativecommons.org/licenses/by/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Casanellas, Ignasi et al., 2020
http://creativecommons.org/licenses/by/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv Articles publicats en revistes (Enginyeria Electrònica i Biomèdica)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300724

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